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Gprasp1 (BC027187) Mouse Tagged ORF Clone
CAT＃: MG211032
- TrueORF®
Gprasp1 (tGFP-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (cDNA clone MGC:25275 IMAGE:4505972)
ORF Plasmid: DDK
"BC027187" in other vectors (4)
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CNY 9,600.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GASP1, GASP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211032 representing BC027187
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAGTGGGCCAAAGGCTACTTCTAAATCTATGCCAGTAGCTAAGGAGGATGAGGTCGTTATTGGGT CCTGGTTCTGGGCAGATGATGAAGAGATCAACTTACAGGCTGATGACGAGTCTATTTTTGGATCTTGGTT CTGGGGCACTGGTGAGAACAGCCTTAGATCTGTTGGAGTCAATTGTGAGAAGATGCCGAAGGCTGGAGAA AAAGAAGTTACTGATTCCTGGTTCTGGGCTGGAGATGTCAATACAGAGGCTGAGGTTGAAGAACAGGCCA GGTCGGCATCTACAAAAGCAACAATCTTTGTGCCTTGGTTTTGGTCTGAAAAGCAGCCGAACATGGATTT AGGGTCTGAACCTTGCTCAGATATCATGGCAGGAGCTGAGGAGGAGCCCATAATTGGGCCCTGGTTCTGG GCTAAAGTAGATAATAGTGTGGAGGCTGAAGTTAACAGTAAGTCTAGCCTTGAGGATGAGGAAGAACCCA TTAGATCCCCTTGGTTTGGGGCCAGAGAACAAACCGATATGAAGTATGCAGCTGGTATCAGATACAAGCC TATGGCAGAAGCTGAGGATGCTAACAAAAAGTCTTGTGTCTGGGCAAAAGAACCCTGTTTGTATCCTACC AATAGAGAATGCTTGAAATCTACTCTGGGAGAAAAAGAAGACACTGTTGATCCATGGCTCTGGTCCAATA ATTATCCAAGGACCAAGACCATCACAGGGTCCTGGCTATGGGCTGCAGAAGAGGGAAATATAGATGATGA GACTGGAGAAAAGATTAAACTACCAACTTTAGAGGACAATGCATTCAATTCTTGGTTCTGGAAAGAAAAT GAAGAAAGCATTGTAGAGGCTCCCAAGAGAGAAGAATTCAGGCCAGAAGCGGAAGAGGAAGACATTATTG GTTCTTGGTTTTGGGCTGGGGATGAAGACAGGTTTGAGCCAGCTGCTAAGATTAATGAAGAGAACAAGAT AGCATCTGAAGATGAAGATACAGTTGGATCCTGGTTCTGGGGCAATGAAGAGGCCAGTCTAGAGGCAGTG AGAAGGGGTACTTTTGAGTCTGCTCCTGGGATTAAGGAAGAGAAAGTTACTGGGTCATGGTTCTGGACTG ATAAGGCCAAAGTAGGGGCTGGGTCCCAGACTGTCGAAACTGGGTCAGAAACTGAAGAGGAAGCAATTTT TGAGTCCTTGATCTGGGCTGCAAAAAAGGACAGTATTCAAGCAGGAGTAAAGCGTGTGTCCAAGCCAAAA GATGACGGTAACATAGCTGTTGGGTCCTGGCTCTGGTCTAGTGACAAGGCCACAAAAGAAGCTAAAACTC TGATTGTCAGTGAGGCCAGTCCAGAAAATGGGAAAGAGTCAGTTGTTAAGTTTGGGTCAAGAGCAAAAGA TGAGGTGATTAACAAGACTGGCAGTGGTGACAATTGTAAACATAGTACAGAAGCTGAAACCATAGTGGGA GCCTGGTTCTGGGAAGGAGATGAAGCTAGCTTTGAATCAAATCCTGTACCTGTATGCAAGGCCGTTTGTG AACCCGAGTCTTCAGCTGAGCACGAACCTGACCCTTCCCGCAGGCCTCAGAGCTGGGATGAGGTCACTGT TCAGTTTAAGGCTGGTCCGTGGGGAAAAGCTGGCTTCCCACCCATGAACCCCTTCAGATTCCCCAAAGAA GCGGCATCTCTGTTTGCAGAAATGTTTGGGGGAAAGCCTAAGCTTGTGGAAGTTGGTCCAGAAAGGGAGC CTGAACCCCAGTTCCCATTTCAGTATGATCCCTCTTACCGCTCAGTCCGAGAAATTCGCGAGCATCTTAA GGCCAGGGAAAGTGCACAGCCAGAGAATTGGTCTTGCAACTGCATCCAGTGTGAGCTTAGAATTGGTTCT GAGGAGTTTGAGGAGCTTCTTTTACTGATGGACAGAAATCGTGATCCTTTTATCCATGAGATATCTAAGA TTGCAATGGGCATGCGAGGTGCTTCTCAGTTTACCCGGGATTTCATTCGAAACTCGGGCGTTGTCTCCCT GATTGAAGCCTTACTTAACTATCCGTCCTCCCGAGTGAGGACAAGGTTTTTGGAAAATATGGTCCGCATG GCTCCCCCTTATCCAGACCTGAACATGATTGAGACATACGTGTGTCAGATTTGCGAGGACACCTTTGACT ATGACTTGGATTCCCCTGATCAGTTGTCCGGATTGACAATGATTACACACCTCACTGCTACTTCTGACTA TCACAAGGTGGTTGTCAATTACTTGGCTGGGTTTTTCTACTTACTGAATTCAGGCAATACCAAAACAAGG TTTCATGTTCTGAAACTGCTACTGAATTTGTCTGAAAACCTTGTCATGACAAAACGCCTACTTGTTACTG ATTCGGTGTCAGAATTTATGGACCTTATTAATAGGGAGGAGTCAGATGAAAACATTCAAATTGTTCTTGC AATATTTGAGACTATCAGTAAACATATTCAAAAAGAGGCACTGTTTTCTGATGATGATGATGATGATGAA GAAGAAGATGCAGTGAATCTTGAACCATTTATTTCTGCATTCCGTGAGGCTGAAAAAATTGCCAAGGAAC TAAAACGCAAACCTGGCAATCAAAAAGCACCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211032 representing BC027187
Red=Cloning site Green=Tags(s) MESGPKATSKSMPVAKEDEVVIGSWFWADDEEINLQADDESIFGSWFWGTGENSLRSVGVNCEKMPKAGE KEVTDSWFWAGDVNTEAEVEEQARSASTKATIFVPWFWSEKQPNMDLGSEPCSDIMAGAEEEPIIGPWFW AKVDNSVEAEVNSKSSLEDEEEPIRSPWFGAREQTDMKYAAGIRYKPMAEAEDANKKSCVWAKEPCLYPT NRECLKSTLGEKEDTVDPWLWSNNYPRTKTITGSWLWAAEEGNIDDETGEKIKLPTLEDNAFNSWFWKEN EESIVEAPKREEFRPEAEEEDIIGSWFWAGDEDRFEPAAKINEENKIASEDEDTVGSWFWGNEEASLEAV RRGTFESAPGIKEEKVTGSWFWTDKAKVGAGSQTVETGSETEEEAIFESLIWAAKKDSIQAGVKRVSKPK DDGNIAVGSWLWSSDKATKEAKTLIVSEASPENGKESVVKFGSRAKDEVINKTGSGDNCKHSTEAETIVG AWFWEGDEASFESNPVPVCKAVCEPESSAEHEPDPSRRPQSWDEVTVQFKAGPWGKAGFPPMNPFRFPKE AASLFAEMFGGKPKLVEVGPEREPEPQFPFQYDPSYRSVREIREHLKARESAQPENWSCNCIQCELRIGS EEFEELLLLMDRNRDPFIHEISKIAMGMRGASQFTRDFIRNSGVVSLIEALLNYPSSRVRTRFLENMVRM APPYPDLNMIETYVCQICEDTFDYDLDSPDQLSGLTMITHLTATSDYHKVVVNYLAGFFYLLNSGNTKTR FHVLKLLLNLSENLVMTKRLLVTDSVSEFMDLINREESDENIQIVLAIFETISKHIQKEALFSDDDDDDE EEDAVNLEPFISAFREAEKIAKELKRKPGNQKAP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC027187 |
| ORF Size | 2622 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC027187.1 |
| RefSeq Size | 3139 bp |
| RefSeq ORF | 2624 bp |
| Locus ID | 67298 |
| Gene Summary | Modulates lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes via its interaction with BECN2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220815 | Gprasp1 (untagged) - Mouse G protein-coupled receptor associated sorting protein 1 (cDNA clone MGC:25275 IMAGE:4505972), (10ug) |
CNY 9,120.00 |
|
| MR211032 | Gprasp1 (Myc-DDK-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (cDNA clone MGC:25275 IMAGE:4505972) |
CNY 8,740.00 |
|
| MR211032L3 | Lenti ORF clone of Gprasp1 (Myc-DDK-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (cDNA clone MGC:25275 IMAGE:4505972) |
CNY 10,640.00 |
|
| MR211032L4 | Lenti ORF clone of Gprasp1 (mGFP-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (cDNA clone MGC:25275 IMAGE:4505972) |
CNY 10,640.00 |
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