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Mastl (NM_025979) Mouse Tagged ORF Clone
CAT＃: MG211003
- TrueORF®
Mastl (tGFP-tagged) - Mouse microtubule associated serine/threonine kinase-like (Mastl)
ORF Plasmid: DDK
"NM_025979" in other vectors (4)
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CNY 8,360.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2700091H24Rik; C88295; GW; GWL; MAST-L; THC2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211003 representing NM_025979
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCCGCCTCGGCAAGTGAGGAGAATGAAGGAGGTGCGGCGATAGAGGAGTGCGTGAGTAGGATCC CGGTACCAAGACCACCCTCCATTGAGGAGTTCACCATAGTGAAGCCCATTAGCCGTGGTGCATTCGGGAA AGTGTACCTGGGACAGAAAGGCGGCAAGTTGTACGCAGTGAAGGTTGTCAAAAAGGCAGACATGATCAAT AAAAATATGACTCATCAGGTACAAGCTGAGAGAGATGCCCTGGCACTGAGCAAAAGCCCTTTTGTTGTGC ATCTCTATTACTCACTGCAGTCAGCAAGCAACATCTACTTGATAATGGAGTATCTTATTGGTGGAGATGT CAAGTCTCTCCTACATATATATGGTTATTTTGATGAAGAGATGGCTATAAAATATATTTCTGAAGTAGCA TTGGCTTTAGACTACCTGCACAGACATGGAATCATCCATAGGGATTTGAAACCGGACAATATGCTTATTT CCAATGAGGGTCATATTAAACTGACAGATTTTGGCCTTTCCAAAGTTACTTTGAATAGAGACATCAATAT GATGGATATTCTCACAACACCATCAATGTCTAAACCTAAGCAAGATTATTCAAGAACTCCAGGACAAGTC TTATCTCTCATCAGTTCTTTGGGATTTTTCACACCAGTTGGAGAAAAAGATCAAGACTCTGCAAACATGT TTTCAGCCCCTAAGTCTGCTGCACAACTTTCTCGAGGATTCATTTGTCCTATGTCTGTAGATCAGAAGGA GCCGACTTCTTATTCGAGCAAACTGCTAAAATCATGTTTTGAAACCCTTTCATCCAATCCAGAAATACCT GTGAAATGTCTGACTTCTAATCTACTCCAGTGTAGGAAAAGACTGGGTACCTCAAGTACTAGTAGCCAGT CTCATACCTTCGTATCCAGTGTGGAGTCAGAATGCCACAGCAATCCCAAATGGGAAAGAGACTGCCAGAG TACCGAATCATCAGGCTGTGCAATGAGCTGGAATGCAGTTGAAATGCTGTATGCAAAATCTACAAGTGCC ATCAAGACAAAAACAGAACTGGAATTAGCACTTTCTCCCATTCATGACAGCAGTGCCATTCCTGCTGCTG GAAGCAACCAGGTGACCCTCCCTAGAAAATGCTTCAGGGAAATCTCTTGGGAAGCAAGGGACCCAGATAA TGAGAACATGACCATTGACAAGGGACAGTCTGGTTTCTGTCAGTCCAGTCAGAGGTCTGTAAATTCTAGT GCTACATCTGAAGAACACCTTGGTAAAAGAAATTATAAAAGAAATTTCCACTTGGTTGACTCCAGTCCTT GTCAGGAAATTATGCAAAGTAAAAAAAACTGTACAGAGTATGAGGCCAATAAGGAAAGGCAAGGCTGCCG TGCAAATCAAAGTACAGGCTTGACAACCGAAGTCCAGAACCTGAAGCTATCAGGGTGTGAAAGTCAGCAA CTTGACTATGCCAATAAGGAGAACATTGTCACCTATTTAACTGACAGACAAACCCCAGAAAAACTGCATA TACCAACGATAGCAAAGAACCTTATGTCTGAACTAGATGAAGACTGTGAGCTGAGCAGTAAAAAGGACTG CCTCAGTTCTAATTCTGTATGTTCTGATGAGGATAGAGCTCTTAAAACTACCTGTGTGGATTCGGATTCA TCTTTTCCTGGAGTGTCTATGATGGAGAGTTCACTGGAAATTCAGGCCTTAGAACCAGATAAAAGCATCA GAGACTACTCTTTTGAAGAACCAAACACTGAAGATCTATTTGTGTTACCAAAATGCCAAGAAAATTCCTT GCCACAAGATGACTGCCATGCTTGTATCCAAGACAGTAGCCAAGTGTCAGCTCATCCCTCAAAGGCGCCC AAAGCATTGACCTCTAAAATCAACGTCGTGGCTTTTCGAAGTTTTAACAGTCATATTAACGCATCTACTA ATTCGGAACCATCCAAAATCAGCATAACTTCTTTAGATGCTATGGACATTTCCTGTGATTACAGTGGTTC ATACCCCATGGCTGTGAGCCCTACTGAGAAAGGAAGGCACTACACGTCACATCAGACTCCAAATCAGGTC AAGTTGGGAACATCATACAGAACTCCAAAGAGTGTGAGAAGAGGGGCAGCCCCAGTAGATGACGGGCGAA TTCTAGGGACTCCAGATTACCTGGCACCTGAGCTCTTACTGGGTACAGCCCATGGTCCTGCAGTAGACTG GTGGGCTCTTGGAGTTTGCTTGTTCGAATTTCTGACAGGAATTCCCCCTTTCAATGATGAAACACCACAA CAAGTATTCCAGAATATTTTAAAAAGAGATATCCCATGGCCAGAAGGCGAAGAAAAGCTATCTGATAATG CTCAAAGTGCAATGGACATGCTTTTAACCATTGATGATTCAAAGAGAGCTGGAATGAGAGAACTAAAACA GCATCCTCTCTTCAGTGAAGTGGACTGGGAAAATCTGCAGCATCAGACTATGCCTTTCGTACCCCAACCA GACGACGAAACAGATACATCCTATTTTGAAGCCAGAAATAATGCTCAACATCTGACCATATCTGGGTTTA GTCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211003 representing NM_025979
Red=Cloning site Green=Tags(s) MESASASEENEGGAAIEECVSRIPVPRPPSIEEFTIVKPISRGAFGKVYLGQKGGKLYAVKVVKKADMIN KNMTHQVQAERDALALSKSPFVVHLYYSLQSASNIYLIMEYLIGGDVKSLLHIYGYFDEEMAIKYISEVA LALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMSKPKQDYSRTPGQV LSLISSLGFFTPVGEKDQDSANMFSAPKSAAQLSRGFICPMSVDQKEPTSYSSKLLKSCFETLSSNPEIP VKCLTSNLLQCRKRLGTSSTSSQSHTFVSSVESECHSNPKWERDCQSTESSGCAMSWNAVEMLYAKSTSA IKTKTELELALSPIHDSSAIPAAGSNQVTLPRKCFREISWEARDPDNENMTIDKGQSGFCQSSQRSVNSS ATSEEHLGKRNYKRNFHLVDSSPCQEIMQSKKNCTEYEANKERQGCRANQSTGLTTEVQNLKLSGCESQQ LDYANKENIVTYLTDRQTPEKLHIPTIAKNLMSELDEDCELSSKKDCLSSNSVCSDEDRALKTTCVDSDS SFPGVSMMESSLEIQALEPDKSIRDYSFEEPNTEDLFVLPKCQENSLPQDDCHACIQDSSQVSAHPSKAP KALTSKINVVAFRSFNSHINASTNSEPSKISITSLDAMDISCDYSGSYPMAVSPTEKGRHYTSHQTPNQV KLGTSYRTPKSVRRGAAPVDDGRILGTPDYLAPELLLGTAHGPAVDWWALGVCLFEFLTGIPPFNDETPQ QVFQNILKRDIPWPEGEEKLSDNAQSAMDMLLTIDDSKRAGMRELKQHPLFSEVDWENLQHQTMPFVPQP DDETDTSYFEARNNAQHLTISGFSL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025979 |
| ORF Size | 2595 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025979.4, NP_080255.3 |
| RefSeq Size | 4652 bp |
| RefSeq ORF | 2598 bp |
| Locus ID | 67121 |
| UniProt ID | Q8C0P0 |
| Gene Summary | Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222270 | Mastl (untagged) - Mouse microtubule associated serine/threonine kinase-like (Mastl), (10ug) |
CNY 11,120.00 |
|
| MR211003 | Mastl (Myc-DDK-tagged) - Mouse microtubule associated serine/threonine kinase-like (Mastl) |
CNY 6,336.00 |
|
| MR211003L3 | Lenti ORF clone of Mastl (Myc-DDK-tagged) - Mouse microtubule associated serine/threonine kinase-like (Mastl) |
CNY 9,500.00 |
|
| MR211003L4 | Lenti ORF clone of Mastl (mGFP-tagged) - Mouse microtubule associated serine/threonine kinase-like (Mastl) |
CNY 9,500.00 |
