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Ago4 (NM_153177) Mouse Tagged ORF Clone
CAT＃: MG210988
- TrueORF®
Ago4 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2C, 4 (Eif2c4)
ORF Plasmid: DDK
"NM_153177" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5730550L01Rik; AI481660; Eif2c4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210988 representing NM_153177
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGCTGGGACCCGGACCTCCAGCCAGCCTCTTCCAGCCACCTCGGCGTCCTGGCCTTGGAACCG TTGGGAAACCAATTCGACTGTTAGCCAATCATTTTCAGGTTCAGATCCCTAAAATAGATGTGTATCACTA TGATGTGGATATTAAACCAGAAAAACGGCCTCGTAGAGTCAACAGGGAGGTGGTAGACACTATGGTGCGG CACTTCAAGATGCAGATATTTGGAGATCGGCAGCCTGGCTACGATGGCAAAAGAAACATGTACACGGCAC ACCCACTGCCAATCGGACGGGATAGGATTGATATGGAGGTGACCCTCCCAGGTGAGGGTAAAGACCAAAC TTTCAAAGTGTCTGTGCAGTGGGTGTCAGTTGTGAGCCTTCAGCTGCTTTTAGAAGCTTTAGCAGGACAC CTGAATGAAGTCCCAGATGACTCAGTACAAGCACTTGATGTGATTACAAGGCATCTCCCCTCTATGAGGT ACACTCCAGTAGGCCGGTCTTTTTTCTCACCTCCTGAAGGTTATTACCACCCTCTGGGAGGGGGCAGAGA GGTTTGGTTTGGCTTTCATCAGTCTGTGAGACCTGCCATGTGGAATATGATGCTCAATATTGATGTATCT GCAACTGCTTTCTACCGGGCTCAACCTATCATTGAGTTCATGTGTGAGGTTTTAGACATTCAGAACATCA ATGAGCAGACAAAACCTCTAACAGACTCCCAGCGTGTCAAGTTTACCAAAGAAATTAGGGGTCTTAAGGT TGAGGTGACTCACTGTGGACAGATGAAACGAAAATATCGAGTTTGTAATGTGACAAGACGGCCAGCCAGT CATCAAACCTTCCCTTTGCAGCTAGAGAATGGCCAAGCTATGGAATGTACAGTGGCTCAGTATTTTAAGC AGAAGTATAGTCTGCAGCTGAAACACCCCCACCTTCCCTGCCTTCAAGTGGGGCAAGAGCAAAAGCATAC CTACCTACCACTTGAGGTCTGTAATATAGTGGCAGGACAGAGATGTATAAAGAAGCTCACAGACAATCAG ACATCCACCATGATCAAAGCCACGGCAAGATCTGCTCCTGACAGACAGGAGGAAATCAGTAGACTGGTGA AGAGCAACAGCATGGTGGGTGGACCTGACCCATACCTGAAGGAATTTGGGATTGTTGTGCACAACGAGAT GACAGAGCTCACAGGCAGGGTGCTTCCGGCTCCAATGCTGCAGTATGGAGGCCGGAATAAAACAGTAGCC ACACCCAGCCAGGGTGTCTGGGACATGCGAGGAAAACAGTTTTATGCTGGCATTGAAATTAAAGTATGGG CAGTGGCTTGTTTTGCGCCTCAGAAACAATGTAGGGAAGATTTGCTAAAGAGTTTCACTGACCAACTTCG TAAAATCTCCAAGGATGCAGGGATGCCCATCCAGGGTCAGCCATGTTTCTGCAAGTATGCTCAAGGTGCA GACAGCGTGGAGCCCATGTTTAAACATCTGAAAATGACATATGTGGGCCTGCAGCTAATAGTGGTGATTT TGCCTGGGAAGACACCAGTATATGCGGAGGTGAAGCGTGTTGGAGATACCCTTCTAGGTATGGCCACACA GTGTGTCCAGGTCAAAAACGTTGTGAAGACCTCGCCCCAGACCCTTTCCAACCTTTGCCTGAAGATGAAC GCAAAGCTGGGAGGGATTAACAATGTGCTTGTGCCTCACCAAAGGCCCTCAGTGTTCCAGCAGCCTGTCA TCTTTCTGGGAGCAGATGTCACTCATCCGCCTGCTGGGGACGGGAAGAAGCCTTCTATTGCAGCCGTGGT GGGCAGCATGGACGGCCATCCCAGCCGGTACTGTGCCACAGTCCGGGTACAGACATCTCGACAGGAGATC ACCCAGGAGCTCCTCTACAGCCAGGAGGTCGTGCAGGACCTGACGAGCATGGCTCGGGAGCTGCTGATTC AATTCTATAAGTCCACGCGCTTCAAGCCCACACGCATCATCTACTACCGCGGAGGGGTATCCGAGGGACA GATGAAGCAGGTAGCTTGGCCGGAGCTAATAGCAATTCGAAAGGCATGTATAAGCTTGGAGGAAGACTAT CGGCCGGGAATAACTTACATTGTGGTGCAAAAGAGGCACCACACACGACTCTTCTGTGCAGATAAAATGG AAAGGGTGGGGAAAAGTGGCAATGTCCCAGCAGGCACCACAGTGGATAGCACCGTCACACACCCGTCTGA GTTTGACTTTTACCTCTGTAGTCATGCAGGAATTCAGGGAACCAGCCGTCCTTCACATTACCAGGTCTTG TGGGATGACAACTGCTTCACTGCAGATGAACTCCAGCTACTGACTTACCAGCTCTGTCACACCTACGTGA GGTGCACACGCTCCGTCTCCATTCCTGCCCCTGCATATTATGCCCGGCTTGTGGCATTCCGGGCAAGGTA TCATTTGGTGGATAAAGATCATGACAGTGCGGAAGGCAGTCACGTGTCAGGACAGAGCAACGGCCGGGAT CCTCAGGCCTTGGCTAAGGCTGTGCAGATCCACCATGATACCCAGCACACTATGTATTTTGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210988 representing NM_153177
Red=Cloning site Green=Tags(s) MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRVNREVVDTMVR HFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRIDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGH LNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVS ATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPAS HQTFPLQLENGQAMECTVAQYFKQKYSLQLKHPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQ TSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVA TPSQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGA DSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKMN AKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEI TQELLYSQEVVQDLTSMARELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDY RPGITYIVVQKRHHTRLFCADKMERVGKSGNVPAGTTVDSTVTHPSEFDFYLCSHAGIQGTSRPSHYQVL WDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRD PQALAKAVQIHHDTQHTMYFA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153177 |
| ORF Size | 2583 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153177.3, NP_694817.2 |
| RefSeq Size | 6527 bp |
| RefSeq ORF | 2586 bp |
| Locus ID | 76850 |
| UniProt ID | Q8CJF8 |
| Gene Summary | Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222171 | Ago4 (untagged) - Mouse eukaryotic translation initiation factor 2C, 4 (Eif2c4), (10ug) |
CNY 10,930.00 |
|
| MR210988 | Ago4 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2C, 4 (Eif2c4) |
CNY 6,312.00 |
|
| MR210988L3 | Lenti ORF clone of Ago4 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2C, 4 (Eif2c4) |
CNY 9,030.00 |
|
| MR210988L4 | Lenti ORF clone of Ago4 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2C, 4 (Eif2c4) |
CNY 9,030.00 |
