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Ube4a (NM_145400) Mouse Tagged ORF Clone
CAT＃: MG210961
- TrueORF®
Ube4a (tGFP-tagged) - Mouse ubiquitination factor E4A, UFD2 homolog (S
ORF Plasmid: DDK
"NM_145400" in other vectors (3)
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CNY 9,790.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4732444G18Rik; 9930123J21Rik; UFD2b |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210961 representing NM_145400
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGAGCAACTCAACATCAATCACATGATCCAGAGGATCTTCCTCATCACCCTGGACAACAGTGATC CAAGCTTGAAAAGCGGGAATGGCATCCCCAGCCGTTGTGTGTACTTGGAAGAAATGGCAGTAGAGCTGGA AGATCAAGACTGGCTTGATATGAGCAATGTTGAGCAGGCCATCTTTGCTCGATTATTGCTTCAAGATCCA GGAAACCACTTGATCAGCATGACTTCCTCCACAACTTTAAATCTCTCTGCTGATCGTGATGCAGGAGAGA GGCACATCTTCTGTTACCTTTACTCCTGCTTCCAGAGAGCCAAGGAAGAGATTACCAAAGTTCCAGAAAA CCTACTCCCCTTTGCAGTGCAGTGCAGAAACCTCACTGTGTCCAACACTCGAACAGTTCTCCTCACCCCA GAGATCTATGTTGACCAAAACATCCATGAGCAACTGGTAGATTTGATGTTGGAAGCTATTCAGGGAGCCC ATTTTGAAGATGTAACTGAATTTTTAGAAGAGGTCATTGAAGCCTTGCTGTTGGATGAGGAGGTTCGAAC ATTTCCTGAAGTCATGATTCCAGTGTTTGATATTTTACTCAGCCGAATAAAAGATCTGGAACTCTGCCAG ATCCTCCTGTATGCATATCTGGACATTCTTCTCTATTTCACTAGGCAGAAGGATATGGCCAAGGTGTTTT TAGAATACATTCAGCCCAAGGATCCCAGCAATGGGCAGATGTATCAGAAGACCTTGCTGGGAGTCATCCT GAATATCTCGTGCTTGTTAAAGACTCCGGGTGTGGTAGAGAACCATGGCTTCTTCCTGAATCCATCCCGT TCCAGTCCTCAGGAGATCAAAGTACAGGAGGCCAACATCCATCAGTTCATGGCTCAGTTCCATGAAAAGA TCTACCAGATGCTGAAGAACTTGCTCCAGCTCTCTCCAGAAACCAAGCACTGTATCTTGTTCTGGCTTGG AAACTGTTTGCATGCAAATGCAGGCCGCACCAAGATCTGGGCCAATCAGATGCCCGAAATCTTCTTCCAA ATGTATGCCTCGGATGCCTTCTTTCTGAATCTGGGTGCTGCCCTCCTGAAGCTGTGCCAGCCATTCTGCA AGCCCAGATCCTCCCGGCTCCTCACCTTTAACCCCACGTACTGTGTCCTCAAGGACTTGAATGATGAAGA ACGAAAAATTAAAAGTGTGCATATGAGAGGTTTGGACAAGGAGACCTGTCTGATCCCAGCTGTGCAGGAG CCAATGTTCCCCCAGAGCTACAACCTTGTGACGGAGAACCTGGCCCTGACAGAGTATACCTTGTACTTGG GATTTCACAGGTTGCATGATCAGATGGTAAAAATCAACCAAAATCTGCATCGGCTGCAGGTTGCCTGGCG GGATGCTCAGCAGAGTTCTAGCCCTGCTGCTGACAATCTCCGTGAGCAGTTCGAACGCCTGATGACCATC TATCTCTCTACCAAGACTGCTATGACTGAACCCCAGATGCTGCAAAACTGTCTGAATTTGCAGGTGTCCA TGGCTGTTCTGCTGGTTCAACTGGCCATAGGCAATGAGGGCTCCCAGCCAATAGAACTAAGTTTCCCTTT GCCAGATGGCTACAGCTCTTTGGCTTATGTGCCAGAATTTTTTGCAGATAACTTGGGTGATTTCCTCATT TTCCTCCGGCGCTTTGCTGAGGACATCTTGGAGACGTCAGCAGACTCCCTGGAGCATGTCCTTCATTTTA TCACCATTTTCACTGGAAGCATAGAAAGAATGAAGAATCCTCACCTGAGGGCCAAACTAGCAGAGGTGTT AGAAGCAGTGATGCCTCACCTAGACCAGACCCCAAGCCCCTTGGTGTCCAGTGTGTTCCACCGGAAACGT GTGTTCTGCAACTTTCCATACGCTCCCCAACTTGCAGAAGCTTTGATCAAGGTGTTTGTGGACATCGAGT TTACAGGGGACCCCCATCAATTTGAACAGAAGTTTAATTACCGCCGTCCTATGTATCCCATCCTAAGATA CATGTGGGGGACAGATTGCTATCGGGAGAGCATTAAGGATTTGGCTGACTATGCCTCTAAGAATTTAGAA GCTATGAATCCCCCACTGTTCCTCCGATTTCTTAACCTGTTAATGAATGATGCTATCTTCCTTTTGGATG AAGCCATACAGTATTTGAGCAAGATAAAGATTCAGCAGATTGAGAAAGACCGAGGAGAATGGGAGAGTCT GACACCAGAAGCCCGCCGAGAGAAAGAGGCAGGTCTGCAGATGTTTGGGCAGCTGGCACGGTTCCATAAC ATCATGTCCAATGAAACAATTGGTACCCTTTCCTTCCTGACATCAGAGATCAAGTCGCTGTTTGTGCATC CGTTCCTGGCCGAGCGCATCATCTCCATGCTCAACTACTTCCTGCAGCACCTGGTGGGCCCCAAGATGGG TGCCCTCAAAGTCAAGGACTTCAGCGAATTTGACTTCAAACCTCAGCAGCTCGTATCAGACATCTGCACT ATCTACTTAAATCTTGGGTATTTGAGATTGGTGTTGAGAAAGCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210961 representing NM_145400
Red=Cloning site Green=Tags(s) MCEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQAIFARLLLQDP GNHLISMTSSTTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNTRTVLLTP EIYVDQNIHEQLVDLMLEAIQGAHFEDVTEFLEEVIEALLLDEEVRTFPEVMIPVFDILLSRIKDLELCQ ILLYAYLDILLYFTRQKDMAKVFLEYIQPKDPSNGQMYQKTLLGVILNISCLLKTPGVVENHGFFLNPSR SSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCILFWLGNCLHANAGRTKIWANQMPEIFFQ MYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCVLKDLNDEERKIKSVHMRGLDKETCLIPAVQE PMFPQSYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQFERLMTI YLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELSFPLPDGYSSLAYVPEFFADNLGDFLI FLRRFAEDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPSPLVSSVFHRKR VFCNFPYAPQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDCYRESIKDLADYASKNLE AMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWESLTPEARREKEAGLQMFGQLARFHN IMSNETIGTLSFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICT IYLNLGYLRLVLRKP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145400 |
| ORF Size | 2565 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145400.2, NP_663375.2 |
| RefSeq Size | 3400 bp |
| RefSeq ORF | 3087 bp |
| Locus ID | 140630 |
| UniProt ID | E9Q735 |
| Gene Summary | Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR210961 | Ube4a (Myc-DDK-tagged) - Mouse ubiquitination factor E4A, UFD2 homolog (S. cerevisiae) (Ube4a) |
CNY 6,272.00 |
|
| MR210961L3 | Lenti ORF clone of Ube4a (Myc-DDK-tagged) - Mouse ubiquitination factor E4A, UFD2 homolog (S. cerevisiae) (Ube4a) |
CNY 10,830.00 |
|
| MR210961L4 | Lenti ORF clone of Ube4a (mGFP-tagged) - Mouse ubiquitination factor E4A, UFD2 homolog (S. cerevisiae) (Ube4a) |
CNY 10,830.00 |
