Dzip1 (BC098211) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	mKIAA0996
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210955 representing BC098211 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTTCCAGAAGCACGTCTACTACCCTCTGGCCAACAGCCCCGAGGGGCCTGACGCCTCTGCCATAG GCGCGGCTCCCATGGCCTTTGTCCCCCCCAGCGCGGCCTCGGGCCCCCTGCCCTTCTTCCAGTTCCGGCC GCGACTGGAGAGCGTGGACTGGAGGCGGCTGAGCGCCATCGACGTGGACAAGGTTGCCGGCGCCGTGGAC GTCCTGACACTGCAGGAGAACATTATGAACATCACCTTCTGCAAGCTGGAGGACGAGAAGTGCCCGCACT GCCAGTCAGGGGTAGACCCAGTGCTGCTGAAGCTGATCCGCCTGGCGCAGCTCACGATCGAATACCTGAT GCACTCGCAGGAGTTCCTCACCTCGCAGCTAAACCTGGTGGAGGAGAGGCTGCGCCTGAGCCTGCTCGAC TATGAGCAGAGCAAGCAGCTGCTCACCAAGCAGGCTGGCGAGATCAAGCTGCTGAAGGAGGAGTGTAAAC GCAGGAAGAAGATGCTGTCCACGCAGCAGCTGATGATAGAGGCCAAGGCCAGCTATTACCAGTGCCATTT CTGTGACAAGGCCTTTATGAACCAAGCGTTCCTACAAAGCCACATCCAGCGCCGCCATACTGAAGACTCT CACCTCGAGTATAATACAAAGGCCCAGACCGACAGGCTCCAGAAGGAGATTGACATGTTGAAGGAACAGC TGCAGCTCACTAGGTCTCAGCTGGAGTCTGCACAGCACTCTCATGCAGTTCGCTTCTCTAAGGACTATGA AATGCAGAAATCAAAAGAGGAAGACTTTCTGAAGCTGTTTGACAGATGGAAAGAGGAGGAGAAGGAGAAG TTACTCGAGGAGATGGAGAAGGTCAAGGGGATGTTTATGAGGGAATTTAAAGAGTTAACTTCTAAGAACT CAGCATTAGAATATCAGTTGTTAGAAATACAGAAGTCTAACATCCAGATCAAGTCCAACATAGGCACCTT AAGAGACGTTACTGAGCTGAGAGAAGACCATCTTCCGTGTCCCCAGGACTTCCAGAACATGCTACAGCTG CTTGACAGCCAGGCAAGCAAGTGGACAGATCGATTTCAAGTTCTCAACGAAGAACACAGCAAGGAAAAGG GGCAGCTCCTGTCCCACATAGAGAAGCTTCGAAGCTCAATGATGAAGGATTTAAGTGCAGATAATGTCTT CTACAAGAGGCGGGTAGAAGAGTTAGGCCAGAAACTGCAGGAGCAGAACGAGCTGATTATCAGTCAGAAG CAGCAGATTAGAGAATTTGCCAGTAAACCATACAGCAGCATCAGCGAGCTGAAAGGGACTCCTTTAACCC GACAAACACTGGAACCTAAATCGGCTGCACCAACCACACCCATGACTGCCTCGGCCACACAGAATCTGGA TGGTGCATCCAGCCTGACCATGGTACATGAGCAAGTGTTCTCTTCACACATACTGGAACCAATAGAAGAA CTTTCTTCAGAGGAGGAAAAAGGTCGAGAAAATGAGCAGAAATTAAATAAAAAGACGTCCTTAAGGAAAC CGTCGAGCACCAGCCCATCCCCCCAGGAGCTGAGGACTAACCTGGAGCGGGAGCTGGGGAATAAACTAAG AAGCTTTGGGATTGGCGCGAACATCCAAGGTATTCCGTGCGAGATCTTGAATAGATCGCTTAAAGCCATG CAAGTTGCCAGACATGACCTAGCAAAACAAATGCCTGACATCCAGCAAATCCGAGAATCCCTCGAGCACC AGCTGATCTGTAAGATGGAGGAAAAAGTGTCACTCTCTTCAGACAGGCACCATGTCCCTTCCATGACCAC CTTTCCACCCGAAGAAGTACCCAAGGCAACCCAGCTTCCCCACAAAAGCAGACCGTTGGTCAGACAGAGA ACTGTGTTTACTGATAAAGTGTCTGTCCCAAAACTTAAGAAAAATACCAAAGAAAGTCATTTTCTCAGAA GGTTTCCATCCACCAAGACGCCTCCCTTCAGCTCGGAAGAGGAGCCAGATGAAGAGGACCTCCTCCATGC TTACCTCTCCCCAGACTCACTTGCCACCGCTGCCACACAGCCACCTAAAAGCAGCATGAGTCACTTTGGA AAGAGCGCTGTCAAAAGTGACACGGACTGGACGGAGGGAAGTGAAATGGATGACTCGGACTTCTCTCCTA AGCTGACAGGAACCTCCATTACGATACAGACTGACACTGTTGAGACCATGGCTTTACCCCAAGGAAGTGG GAATAAAGCAGTGCCGGGAATGAACCCTGCTGACACGGTTATCAAAAAGGAATCGTTGCAAGAGTTAAAG TGCACAGATGCTGATGACGAGGACTGGGATATATCGTCCTTAGAGGAAGAGAAGTCTCTGGGGAGTAAAA TAGAGCAGAGGGAGCCGCCTCCGGCCAAGCGGGACCCCAGCTGCACCCAGGTGCAGAGGGCCTGGGGCCC AGTGAACCCTCGGGAATTCAAGGAAGAAGGACTTCATGAAAACGAACCGAGCACACTCAAGAGCAACTTA GTCACTGTGACTGACTGGAGCGATGTCCTGGATGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210955 representing BC098211 Red=Cloning site Green=Tags(s) MPFQKHVYYPLANSPEGPDASAIGAAPMAFVPPSAASGPLPFFQFRPRLESVDWRRLSAIDVDKVAGAVD VLTLQENIMNITFCKLEDEKCPHCQSGVDPVLLKLIRLAQLTIEYLMHSQEFLTSQLNLVEERLRLSLLD YEQSKQLLTKQAGEIKLLKEECKRRKKMLSTQQLMIEAKASYYQCHFCDKAFMNQAFLQSHIQRRHTEDS HLEYNTKAQTDRLQKEIDMLKEQLQLTRSQLESAQHSHAVRFSKDYEMQKSKEEDFLKLFDRWKEEEKEK LLEEMEKVKGMFMREFKELTSKNSALEYQLLEIQKSNIQIKSNIGTLRDVTELREDHLPCPQDFQNMLQL LDSQASKWTDRFQVLNEEHSKEKGQLLSHIEKLRSSMMKDLSADNVFYKRRVEELGQKLQEQNELIISQK QQIREFASKPYSSISELKGTPLTRQTLEPKSAAPTTPMTASATQNLDGASSLTMVHEQVFSSHILEPIEE LSSEEEKGRENEQKLNKKTSLRKPSSTSPSPQELRTNLERELGNKLRSFGIGANIQGIPCEILNRSLKAM QVARHDLAKQMPDIQQIRESLEHQLICKMEEKVSLSSDRHHVPSMTTFPPEEVPKATQLPHKSRPLVRQR TVFTDKVSVPKLKKNTKESHFLRRFPSTKTPPFSSEEEPDEEDLLHAYLSPDSLATAATQPPKSSMSHFG KSAVKSDTDWTEGSEMDDSDFSPKLTGTSITIQTDTVETMALPQGSGNKAVPGMNPADTVIKKESLQELK CTDADDEDWDISSLEEEKSLGSKIEQREPPPAKRDPSCTQVQRAWGPVNPREFKEEGLHENEPSTLKSNL VTVTDWSDVLDV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC098211
ORF Size	2558 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC098211, AAH98211
RefSeq Size	4097 bp
RefSeq ORF	2558 bp
Locus ID	66573
Gene Summary	This gene encodes a zinc finger protein that has been demonstrated to interact with the deleted in azoospermia (DAZ) protein. DAZ plays an important role early in germ cell development to maintain the initial germ cell population. Deletion of this gene in mice compromises Hedgehog signaling during embryogenesis. Mouse embryos lacking the encoded protein show severe developmental defects with dorsalized neural tubes and underdeveloped somites. Alternative splicing of this gene results in multiple transcript variants. A pseudogene for this gene has been identified on chromosome 5. [provided by RefSeq, Jan 2015]

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