Prom1 (BC028286) Mouse Tagged ORF Clone
CAT#: MG210921
- TrueORF®
Prom1 (tGFP-tagged) - Mouse prominin 1 (cDNA clone MGC:25280 IMAGE:4502359)
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CNY 9,220.00
货期*
4周
规格
Cited in 1 publication. |
经常一起买 (5)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Prom, AC133, CD133, Proml1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210921 representing BC028286
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTCGTCTTCAGTGCCCTGCTGTTACTGGGGCTGTGTGGAAAGATCTCTTCAGAAGGTCAGCCTG CATTCCATAACACTCCTGGGGCTATGAATTATGAATTGCCTACCACCAAATATGAGACCCAAGATACCTT CAATGCTGGGATTGTTGGCCCTCTCTACAAAATGGTGCACATCTTCCTCAACGTGGTCCAGCCGAATGAC TTCCCTCTAGATTTGATCAAAAAACTCATACAGAACAAGAACTTTGACATCTCAGTTGATTCCAAGGAGC CAGAAATCATAGTCTTGGCTCTGAAGATTGCCCTCTATGAGATCGGAGTCCTTATCTGCGCCATCCTGGG ACTGCTGTTCATTATCCTCATGCCTCTGGTGGGCTGCTTCTTTTGTATGTGCCGTTGCTGCAACAAATGC GGCGGAGAGATGCACCAGCGGCAGAAGCAGAATGCGCCATGCAGGAGGAAGTGCTTGGGCCTCTCCCTCC TGGTGATTTGTCTGCTCATGAGCCTTGGCATTATATATGGCTTTGTGGCTAACCAGCAGACCAGGACTCG GATCAAAGGGACCCAGAAACTGGCAAAGAGCAATTTCAGAGACTTTCAAACACTCCTGACTGAAACACCA AAGCAAATTGACTATGTAGTGGAGCAGTACACCAACACCAAGAACAAGGCATTCTCAGACCTGGATGGCA TCGGCTCCGTGCTGGGAGGCAGAATAAAGGACCAACTAAAACCCAAAGTAACTCCTGTCCTCGAAGAGAT TAAGGCCATGGCGACAGCCATCAAACAGACCAAGGATGCCCTGCAGAACATGAGCAGCAGCCTGAAAAGT CTCCAAGATGCAGCCACCCAGCTCAATACCAACCTGAGCTCTGTGAGAAACAGCATCGAGAATTCGCTCA GCAGCAGTGACTGTACCTCAGATCCAGCCAGCAAGATCTGCGATAGCATCAGACCAAGCCTAAGCAGTCT GGGGAGCAGCCTCAATTCAAGTCAGCTCCCATCAGTGGATAGAGAACTCAACACTGTTACTGAAGTCGAC AAAACTGATCTGGAGAGCCTCGTCAAAAGGGGGTATACGACAATTGATGAAATACCCAATACAATACAAA ACCAAACTGTGGATGTCATCAAAGACGTCAAAAATACCTTGGACTCCATTAGCTCCAACATTAAGGACAT GAGCCAAAGTATTCCTATTGAGGATATGCTGTTACAGGTCTCCCATTACCTTAATAACAGCAACAGATAC TTAAACCAGGAGCTGCCCAAGCTGGAAGAATATGACTCGTACTGGTGGCTGGGTGGCTTGATTGTCTGCT TTCTGCTGACTCTCATTGTGACCTTCTTTTTCCTGGGCTTGCTGTGTGGTGTGTTTGGCTATGACAAGCA TGCCACCCCAACTAGAAGAGGCTGTGTGTCCAACACTGGAGGCATCTTCCTCATGGCTGGGGTTGGATTC GGCTTCCTTTTTTGCTGGATATTGATGATCCTTGTGGTTCTTACGTTTGTTGTTGGTGCAAATGTGGAAA AGTTGCTCTGCGAACCTTATGAAAACAAGAAATTATTACAGGTTTTGGACACTCCCTATCTGCTCAAGGA ACAATGGCAATTTTATCTTTCTGGCATGCTATTCAATAACCCAGACATTAACATGACCTTTGAGCAAGTC TACAGGGATTGCAAAAGAGGTCGAGGTATATATGCTGCTTTTCAGCTTGAGAATGTCGTCAACGTCAGTG ATCATTTCAACATTGACCAGATTTCTGAAAACATAAATACGGAGTTGGAAAACCTGAATGTGAACATTGA TAGCATTGAACTGTTGGATAACACAGGAAGGAAGAGCCTCGAGGACTTTGCACATTCTGGGATAGATACA ATCGATTATTCCACATACTTGAAGGAGACTGAGAAATCCCCTACTGAAGTGAATCTGCTGACATTTGCCT CTACCCTGGAAGCAAAAGCAAACCAGTTGCCTGAAGGAAAGCTGAAACAGGCCTTCTTACTGGATGTACA GAATATAAGAGCCATCCACCAGCATCTCCTCCCTCCTGTGCAGCAATCACTGAAATTTGTGAGGGTGAGG AATACGTTAAGACAAAGTGTCTGGACCCTCCAGCAAACAAGCAACAAGTTGCCGGAGAAAGTGAAGAAGA TCCTTGCCTCTTTGGACTCTGTTCAGCATTTCCTCACCAATAACGTTTCCCTCATCGTTATCGGGGAAAC GAAGAAGTTTGGGAAAACAATACTAGGCTACTTTGAACATTATCTGCACTGGGTCTTTTATGCCATCACA GAGAAGATGACATCCTGCAAACCCATGGCCACCGCGATGGACTCTGCTGTTAATGGCATTCTGTGTGGCT ATGTTGCGGACCCTCTGAATTTGTTCTGGTTCGGCATAGGGAAAGCCACGGTGCTCTTACTTCCGGCTGT AATCATTGCTATCAAGCTGGCCAAGTACTATCGCAGGATGGATTCAGAGGATGTATACGACGACCCGTCT CGATAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210921 representing BC028286
Red=Cloning site Green=Tags(s) MALVFSALLLLGLCGKISSEGQPAFHNTPGAMNYELPTTKYETQDTFNAGIVGPLYKMVHIFLNVVQPND FPLDLIKKLIQNKNFDISVDSKEPEIIVLALKIALYEIGVLICAILGLLFIILMPLVGCFFCMCRCCNKC GGEMHQRQKQNAPCRRKCLGLSLLVICLLMSLGIIYGFVANQQTRTRIKGTQKLAKSNFRDFQTLLTETP KQIDYVVEQYTNTKNKAFSDLDGIGSVLGGRIKDQLKPKVTPVLEEIKAMATAIKQTKDALQNMSSSLKS LQDAATQLNTNLSSVRNSIENSLSSSDCTSDPASKICDSIRPSLSSLGSSLNSSQLPSVDRELNTVTEVD KTDLESLVKRGYTTIDEIPNTIQNQTVDVIKDVKNTLDSISSNIKDMSQSIPIEDMLLQVSHYLNNSNRY LNQELPKLEEYDSYWWLGGLIVCFLLTLIVTFFFLGLLCGVFGYDKHATPTRRGCVSNTGGIFLMAGVGF GFLFCWILMILVVLTFVVGANVEKLLCEPYENKKLLQVLDTPYLLKEQWQFYLSGMLFNNPDINMTFEQV YRDCKRGRGIYAAFQLENVVNVSDHFNIDQISENINTELENLNVNIDSIELLDNTGRKSLEDFAHSGIDT IDYSTYLKETEKSPTEVNLLTFASTLEAKANQLPEGKLKQAFLLDVQNIRAIHQHLLPPVQQSLKFVRVR NTLRQSVWTLQQTSNKLPEKVKKILASLDSVQHFLTNNVSLIVIGETKKFGKTILGYFEHYLHWVFYAIT EKMTSCKPMATAMDSAVNGILCGYVADPLNLFWFGIGKATVLLLPAVIIAIKLAKYYRRMDSEDVYDDPS RY TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC028286 |
ORF Size | 2528 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC028286, AAH28286 |
RefSeq Size | 3701 bp |
RefSeq ORF | 2528 bp |
Locus ID | 19126 |
Gene Summary | May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis (PubMed:19228982). Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
CD133 Promotes Adhesion to the Ovarian Cancer Metastatic Niche
,null,
Cancer Growth and Metastasis
,PubMed ID 29662326
[Prom1]
|
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