Prom1 (BC028286) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Prom, AC133, CD133, Proml1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210921 representing BC028286 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTCGTCTTCAGTGCCCTGCTGTTACTGGGGCTGTGTGGAAAGATCTCTTCAGAAGGTCAGCCTG CATTCCATAACACTCCTGGGGCTATGAATTATGAATTGCCTACCACCAAATATGAGACCCAAGATACCTT CAATGCTGGGATTGTTGGCCCTCTCTACAAAATGGTGCACATCTTCCTCAACGTGGTCCAGCCGAATGAC TTCCCTCTAGATTTGATCAAAAAACTCATACAGAACAAGAACTTTGACATCTCAGTTGATTCCAAGGAGC CAGAAATCATAGTCTTGGCTCTGAAGATTGCCCTCTATGAGATCGGAGTCCTTATCTGCGCCATCCTGGG ACTGCTGTTCATTATCCTCATGCCTCTGGTGGGCTGCTTCTTTTGTATGTGCCGTTGCTGCAACAAATGC GGCGGAGAGATGCACCAGCGGCAGAAGCAGAATGCGCCATGCAGGAGGAAGTGCTTGGGCCTCTCCCTCC TGGTGATTTGTCTGCTCATGAGCCTTGGCATTATATATGGCTTTGTGGCTAACCAGCAGACCAGGACTCG GATCAAAGGGACCCAGAAACTGGCAAAGAGCAATTTCAGAGACTTTCAAACACTCCTGACTGAAACACCA AAGCAAATTGACTATGTAGTGGAGCAGTACACCAACACCAAGAACAAGGCATTCTCAGACCTGGATGGCA TCGGCTCCGTGCTGGGAGGCAGAATAAAGGACCAACTAAAACCCAAAGTAACTCCTGTCCTCGAAGAGAT TAAGGCCATGGCGACAGCCATCAAACAGACCAAGGATGCCCTGCAGAACATGAGCAGCAGCCTGAAAAGT CTCCAAGATGCAGCCACCCAGCTCAATACCAACCTGAGCTCTGTGAGAAACAGCATCGAGAATTCGCTCA GCAGCAGTGACTGTACCTCAGATCCAGCCAGCAAGATCTGCGATAGCATCAGACCAAGCCTAAGCAGTCT GGGGAGCAGCCTCAATTCAAGTCAGCTCCCATCAGTGGATAGAGAACTCAACACTGTTACTGAAGTCGAC AAAACTGATCTGGAGAGCCTCGTCAAAAGGGGGTATACGACAATTGATGAAATACCCAATACAATACAAA ACCAAACTGTGGATGTCATCAAAGACGTCAAAAATACCTTGGACTCCATTAGCTCCAACATTAAGGACAT GAGCCAAAGTATTCCTATTGAGGATATGCTGTTACAGGTCTCCCATTACCTTAATAACAGCAACAGATAC TTAAACCAGGAGCTGCCCAAGCTGGAAGAATATGACTCGTACTGGTGGCTGGGTGGCTTGATTGTCTGCT TTCTGCTGACTCTCATTGTGACCTTCTTTTTCCTGGGCTTGCTGTGTGGTGTGTTTGGCTATGACAAGCA TGCCACCCCAACTAGAAGAGGCTGTGTGTCCAACACTGGAGGCATCTTCCTCATGGCTGGGGTTGGATTC GGCTTCCTTTTTTGCTGGATATTGATGATCCTTGTGGTTCTTACGTTTGTTGTTGGTGCAAATGTGGAAA AGTTGCTCTGCGAACCTTATGAAAACAAGAAATTATTACAGGTTTTGGACACTCCCTATCTGCTCAAGGA ACAATGGCAATTTTATCTTTCTGGCATGCTATTCAATAACCCAGACATTAACATGACCTTTGAGCAAGTC TACAGGGATTGCAAAAGAGGTCGAGGTATATATGCTGCTTTTCAGCTTGAGAATGTCGTCAACGTCAGTG ATCATTTCAACATTGACCAGATTTCTGAAAACATAAATACGGAGTTGGAAAACCTGAATGTGAACATTGA TAGCATTGAACTGTTGGATAACACAGGAAGGAAGAGCCTCGAGGACTTTGCACATTCTGGGATAGATACA ATCGATTATTCCACATACTTGAAGGAGACTGAGAAATCCCCTACTGAAGTGAATCTGCTGACATTTGCCT CTACCCTGGAAGCAAAAGCAAACCAGTTGCCTGAAGGAAAGCTGAAACAGGCCTTCTTACTGGATGTACA GAATATAAGAGCCATCCACCAGCATCTCCTCCCTCCTGTGCAGCAATCACTGAAATTTGTGAGGGTGAGG AATACGTTAAGACAAAGTGTCTGGACCCTCCAGCAAACAAGCAACAAGTTGCCGGAGAAAGTGAAGAAGA TCCTTGCCTCTTTGGACTCTGTTCAGCATTTCCTCACCAATAACGTTTCCCTCATCGTTATCGGGGAAAC GAAGAAGTTTGGGAAAACAATACTAGGCTACTTTGAACATTATCTGCACTGGGTCTTTTATGCCATCACA GAGAAGATGACATCCTGCAAACCCATGGCCACCGCGATGGACTCTGCTGTTAATGGCATTCTGTGTGGCT ATGTTGCGGACCCTCTGAATTTGTTCTGGTTCGGCATAGGGAAAGCCACGGTGCTCTTACTTCCGGCTGT AATCATTGCTATCAAGCTGGCCAAGTACTATCGCAGGATGGATTCAGAGGATGTATACGACGACCCGTCT CGATAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210921 representing BC028286 Red=Cloning site Green=Tags(s) MALVFSALLLLGLCGKISSEGQPAFHNTPGAMNYELPTTKYETQDTFNAGIVGPLYKMVHIFLNVVQPND FPLDLIKKLIQNKNFDISVDSKEPEIIVLALKIALYEIGVLICAILGLLFIILMPLVGCFFCMCRCCNKC GGEMHQRQKQNAPCRRKCLGLSLLVICLLMSLGIIYGFVANQQTRTRIKGTQKLAKSNFRDFQTLLTETP KQIDYVVEQYTNTKNKAFSDLDGIGSVLGGRIKDQLKPKVTPVLEEIKAMATAIKQTKDALQNMSSSLKS LQDAATQLNTNLSSVRNSIENSLSSSDCTSDPASKICDSIRPSLSSLGSSLNSSQLPSVDRELNTVTEVD KTDLESLVKRGYTTIDEIPNTIQNQTVDVIKDVKNTLDSISSNIKDMSQSIPIEDMLLQVSHYLNNSNRY LNQELPKLEEYDSYWWLGGLIVCFLLTLIVTFFFLGLLCGVFGYDKHATPTRRGCVSNTGGIFLMAGVGF GFLFCWILMILVVLTFVVGANVEKLLCEPYENKKLLQVLDTPYLLKEQWQFYLSGMLFNNPDINMTFEQV YRDCKRGRGIYAAFQLENVVNVSDHFNIDQISENINTELENLNVNIDSIELLDNTGRKSLEDFAHSGIDT IDYSTYLKETEKSPTEVNLLTFASTLEAKANQLPEGKLKQAFLLDVQNIRAIHQHLLPPVQQSLKFVRVR NTLRQSVWTLQQTSNKLPEKVKKILASLDSVQHFLTNNVSLIVIGETKKFGKTILGYFEHYLHWVFYAIT EKMTSCKPMATAMDSAVNGILCGYVADPLNLFWFGIGKATVLLLPAVIIAIKLAKYYRRMDSEDVYDDPS RY TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC028286
ORF Size	2528 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC028286, AAH28286
RefSeq Size	3701 bp
RefSeq ORF	2528 bp
Locus ID	19126
Gene Summary	May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis (PubMed:19228982). Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.[UniProtKB/Swiss-Prot Function]

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