Axin2 (BC057338) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Axi1, Conductin, Axil
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210915 representing BC057338 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAGCGCCGTGTTAGTGACTCTCCTTCCAGATCCCAGCAGCAGCTTCCGCGAGGATGCTCCGCGGC CCCCGGTTCCGGGAGAAGAAGGGGAGACCCCACCGTGTCAGCCTAGTGTGGGCAAGGTCCAGTCCACCAA ACCTATGCCCGTTTCCTCTAATGCTAGGCGGAATGAAGATGGACTGGGGGAGCCCGAGGGGCGGGCCTCC CCCGATTCCCCTTTGACCAGGTGGACCAAGTCTTTACACTCCTTGTTGGGTGACCAGGATGGTGCATACC TCTTCCGGACTTTCCTGGAGAGGGAGAAATGTGTGGATACGCTGGACTTCTGGTTTGCTTGTAATGGGTT CAGGCAGATGAACCTGAAGGATACCAAAACTTTGCGAGTGGCCAAAGCAATCTATAAGAGGTACATTGAG AACAACAGCGTTGTCTCCAAGCAGCTGAAGCCCGCCACCAAGACCTACATACGAGATGGCATCAAGAAGC AACAGATCGGCTCGGTCATGTTTGACCAGGCACAGACCGAGATCCAGGCAGTGATGGAGGAAAATGCCTA CCAGGTGTTCTTGACTTCTGACATTTACCTGGAATATGTGAGGAGTGGGGGGGAAAACACAGCTTACATG AGTAACGGGGGACTGGGGAGCCTAAAGGTCTTATGTGGCTACCTCCCCACCTTGAATGAAGAAGAGGAGT GGACGTGTGCCGACCTCAAGTGCAAACTCTCACCCACCGTGGTTGGCTTGTCCAGCAAAACTCTTCGGGC CACCGCGAGTGTGAGATCCACGGAAACAGCTGAAAACGGATTCAGGTCCTTCAAGAGAAGCGACCCAGTC AATCCTTATCACGTAGGTTCCGGCTATGTCTTTGCACCAGCCACCAGCGCCAACGACAGCGAGTTATCCA GCGACGCACTGACCGACGATTCCATGTCCATGACGGACAGTAGCGTAGATGGAGTCCCTCCTTACCGCAT GGGGAGTAAGAAACAGCTCCAGAGAGAGATGCATCGCAGTGTGAAGGCCAATGGCCAAGTGTCTCTACCT CATTTTCCGAGAACCCACCGCCTGCCCAAGGAGATGACGCCTGTGGAACCTGCTGCCTTCGCCGCCGAGC TCATCTCCAGGCTGGAGAAACTGAAACTGGAGCTGGAAAGCCGCCATAGTCTGGAGGAGCGGCTGCAGCA GATCCGGGAGGATGAAGAAAAGGAGGGGTCTGAGCAGGCCCTGAGCTCACGGGATGGAGCACCGGTCCAG CACCCCCTGGCCCTCCTACCCTCCGGCAGCTATGAAGAGGACCCACAAACCATTTTGGACGACCACCTCT CCAGGGTCCTCAAGACCCCCGGCTGTCAATCCCCTGGTGTGGGTCGCTATAGCCCACGGTCCCGCTCCCC CGACCACCACCACCAGCACCACCACCATCAGCAGTGTCATACCCTTCTTCCGACTGGGGGCAAGCTGCCC CCCGTGGCTGCTTGCCCCCTCCTTGGAGGCAAGAGCTTCCTGACCAAACAGACGACGAAGCACGTTCACC ACCACTACATCCACCACCACGCTGTCCCCAAGACCAAGGAGGAGATCGAGGCAGAAGCCACACAGAGAGT CCGCTGCCTCTGTCCTGGGGGAACAGATTATTATTGCTACTCCAAATGCAAAAGCCACCCAAAGGCTCCA GAGCCCCTGCCTGGGGAGCAGTTTTGTGGCAGCAGAGGTGGTACCTTGCCAAAACGGAATGCAAAGGGCA CCGAACCGGGTCTTGCACTGTCGGCCAGGGATGGAGGGATGTCCAGTGCAGCGGGGGCCCCCCAGCTTCC TGGGGAAGAAGGAGACCGGTCACAGGATGTCTGGCAGTGGATGTTAGAGAGTGAGCGGCAGAGCAAGTCC AAGCCCCATAGTGCCCAAAGCATAAGAAAGAGCTACCCATTGGAGTCTGCCTGTGCGGCCCCAGGAGAAC GAGTCAGCCGGCACCATCTGTTGGGGGCCAGCGGACACTCCCGCTCGGTGGCCCGGGCTCACCCATTTAC CCAGGACCCTGCAATGCCTCCCCTTACCCCACCCAACACTTTGGCACAGCTAGAGGAAGCCTGCCGCAGG CTGGCAGAGGTGTCGAAGCCCCAGAAGCAGCGGTGCTGCGTGGCCAGTCAGCAGAGGGACAGGAACCACT CGGCTGCTGGTCAGGCAGGAGCCTCACCCTTCGCCAACCCAAGCCTGGCTCCAGAAGATCACAAAGAGCC AAAGAAACTGGCAAGTGTCCACGCGCTCCAGGCCAGTGAGCTGGTTGTCACCTACTTTTTCTGTGGAGAA GAAATTCCATACAGGAGGATGCTGAAGGCTCAAAGCTTGACCCTGGGCCACTTCAAGGAGCAGCTCAGCA AAAAGGGAAATTACAGGTATTATTTCAAGAAGGCGAGTGACGAATTTGCCTGCGGAGCAGTTTTTGAGGA GATCTGGGACGACGAGACAGTGCTCCCCATGTACGAAGGCAGGATCCTGGGCAAAGTGGAGAGGATCGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210915 representing BC057338 Red=Cloning site Green=Tags(s) MSSAVLVTLLPDPSSSFREDAPRPPVPGEEGETPPCQPSVGKVQSTKPMPVSSNARRNEDGLGEPEGRAS PDSPLTRWTKSLHSLLGDQDGAYLFRTFLEREKCVDTLDFWFACNGFRQMNLKDTKTLRVAKAIYKRYIE NNSVVSKQLKPATKTYIRDGIKKQQIGSVMFDQAQTEIQAVMEENAYQVFLTSDIYLEYVRSGGENTAYM SNGGLGSLKVLCGYLPTLNEEEEWTCADLKCKLSPTVVGLSSKTLRATASVRSTETAENGFRSFKRSDPV NPYHVGSGYVFAPATSANDSELSSDALTDDSMSMTDSSVDGVPPYRMGSKKQLQREMHRSVKANGQVSLP HFPRTHRLPKEMTPVEPAAFAAELISRLEKLKLELESRHSLEERLQQIREDEEKEGSEQALSSRDGAPVQ HPLALLPSGSYEEDPQTILDDHLSRVLKTPGCQSPGVGRYSPRSRSPDHHHQHHHHQQCHTLLPTGGKLP PVAACPLLGGKSFLTKQTTKHVHHHYIHHHAVPKTKEEIEAEATQRVRCLCPGGTDYYCYSKCKSHPKAP EPLPGEQFCGSRGGTLPKRNAKGTEPGLALSARDGGMSSAAGAPQLPGEEGDRSQDVWQWMLESERQSKS KPHSAQSIRKSYPLESACAAPGERVSRHHLLGASGHSRSVARAHPFTQDPAMPPLTPPNTLAQLEEACRR LAEVSKPQKQRCCVASQQRDRNHSAAGQAGASPFANPSLAPEDHKEPKKLASVHALQASELVVTYFFCGE EIPYRRMLKAQSLTLGHFKEQLSKKGNYRYYFKKASDEFACGAVFEEIWDDETVLPMYEGRILGKVERID TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC057338
ORF Size	2522 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC057338, AAH57338
RefSeq Size	4263 bp
RefSeq ORF	2522 bp
Locus ID	12006
Gene Summary	Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.[UniProtKB/Swiss-Prot Function]

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