Ttk (BC058851) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	PYT, esk, Esk1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210857 representing BC058851 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCTGAAGAGTTAATTGGCAGCAGTGTGACGATTGATTCCATCATGAGCAAAATGAGAGATATTA AAAATAAGATAAATGAAGACTGTACTGATGAGCTAAGCTTGTCTAAAATCTGTGCCGATCACACGGAAAC TGTTAACCAAATTATGAGGGTTGGGAACACCCCAGAGAACTGGTTGAATTTCTTGCTGAAACTAGAGAAA AACAGCTCACCTCTAAATGACGATCTTTTAAATAAGCTGATTGGTCGGTATAGTCAAGCGATTGAAGTAC TTCCTCCAGATAAATACGGCCAGAATGAGAGCTTTGCTCGAATACAAGTGAGACTTGCTGAACTAAAAGC TATTCAAGAGCCTGATGATGCCCGTGACTACTTCCAGATGGCCAGGGAAAACTGCAAGAAGTTTGCTTTT GTGCACGTATCTTTTGCACAGTTTGAACTGTCTCAAGGCAATCTTAAAAAAAGTGAGCAGCTTCTTCATA AAGCTGTAGAGACTGGGGCGGTGCCGCTGCAGATGCTGGAGACGGCCATGCGTAACTTACACCTCCAGAA AAAGCAGCTGCTTCCGGAGGAGGACAAGAAGAGTGTGTCAGCATCGACAGTACTAAGTGCCCAAGAGCCG TTCTCCAGCTCACTTGGAAATGTACAGAATCGGAGCATCAGCTGTGAGTCCAGAGGACAGGCTGGGGCAG CCAGGGTTTTATATGGAGAGAACTTGCCTCCACAAGATGCCGAAGTGAGGCATCAAAACCCCTTCAAGCA GACTCACGCAGCTAAACGGTCATGCCCCTTTGGAAGAGTCCCAGTCAATCTTCTAAACAGCCCAGATTTC TATGTGAAGACAGATAGCTCAGCTGTGACACAGTTAACAACAAGAAAGGGGTCGGGACCAGACCGAGACG CGATTCTGCCCGGCTCCAGACCACGTGGCAGTGATTCCTATGAACTGAGAGGTTTAAAGCCCATTCAAAC TATCTATTTGAAAGACTCTTTGGTGTCCAATGAAAAGAGTTCTGAACTTATGTCTGATTTAATAGCCTTG AAGAGTAAAACAGATTCAAGTCTAACAAAATTGGAAGAAACTAAGCCAGAGATTGCAGAAAGAAGGCCCA TGCAGTGGCAGTCTACCAGAAAGCCCGAGTGTGTGTTCCAGAACCCTGCTGCCTTTGCACCCCTGCGGCA CGTTCCAGATGTCACCCTGAAGGCTGACAAAGAGTCACCACCAATATCAGTTCCTAAATGGCTTGATCCA AAGTCTGCTTGTGAGACACCTAGTAGCAGCTCCTTGGATGATTACATGAAATGTTTTAAGACTCCAGTTG TAAAGAATGACTTTCCACCTGCCTGTCCATCATCAACACCTTACAGCCAGCTTGCCCGCCTCCAGCAGCA ACAGCAGCAGGGACTCAGCACTCCTCTTCAAAGCTTGCAGATTTCAGGTTCTTCATCAATAAATGAATGC ATTTCAGTTAACGGAAGAATTTATTCCATATTAAAGCAGATAGACAGTGGAGGTTCCAGTAAGGTGTTTC AGGTATTGAATGAGAAAAAACAGATAAACGCTATCAAATATGTGAACCTAGAAGACGCCGATAGCCAAAC TATTGAGAGCTACCGCAACGAGATAGCGTTTTTGAACAAACTACAGCAACACAGTGATAAGATCATCCGC CTCTATGATTATGAAATCACCGAGCAGTACATCTACATGGTAATGGAATGTGGAAACATTGACCTAAATA GTTGGCTTAAAAAGAAAAAATCCATCAATCCATGGGAACGCAAGAGCTACTGGAAAAACATGTTGGAGGC AGTACACATAATCCATCAGCATGGTATTGTTCATAGTGATCTGAAGCCTGCTAACTTTGTGATAGTGGAT GGAATGCTAAAGCTAATTGATTTTGGGATTGCAAACCAAATGCAGCCAGACACAACAAGCATTGTTAAAG ATTCTCAGGTTGGCACAGTTAACTATATGGCCCCAGAAGCAATCAGAGACATGTCTTCTTCAAGAGAAAA TTCGAAAATCAGAACCAAGGTAAGTCCCAGAAGTGATGTCTGGTCCTTGGGGTGCATTTTGTACTACATG ACTTATGGGAGGACGCCATTTCAGCACATCATCAATCAGGTCTCTAAACTGCACGCCATAATCAACCCTG CTCATGAGATTGAATTTCCCGAGATTTCGGAAAAAGATCTTCGAGACGTGCTAAAGTGCTGTTTAGTGAG GAACCCTAAAGAGAGGATATCTATCCCTGAGCTCCTCACACATCCGTATGTTCAAATTCAGCCCCATCCA GGCAGCCAAATGGCTAGGGGAGCCACTGATGAAATGAAATATGTGTTGGGTCAACTTGTTGGTCTGAATT CTCCTAACTCCATCTTGAAAACTGCAAAAACTTTGTATGAACGTTATAATTGTGGTGAAGGTCAAGATTC TTCGTCATCCAAGACTTTTGACAAAAAGAGAGAAAGAAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210857 representing BC058851 Red=Cloning site Green=Tags(s) MEAEELIGSSVTIDSIMSKMRDIKNKINEDCTDELSLSKICADHTETVNQIMRVGNTPENWLNFLLKLEK NSSPLNDDLLNKLIGRYSQAIEVLPPDKYGQNESFARIQVRLAELKAIQEPDDARDYFQMARENCKKFAF VHVSFAQFELSQGNLKKSEQLLHKAVETGAVPLQMLETAMRNLHLQKKQLLPEEDKKSVSASTVLSAQEP FSSSLGNVQNRSISCESRGQAGAARVLYGENLPPQDAEVRHQNPFKQTHAAKRSCPFGRVPVNLLNSPDF YVKTDSSAVTQLTTRKGSGPDRDAILPGSRPRGSDSYELRGLKPIQTIYLKDSLVSNEKSSELMSDLIAL KSKTDSSLTKLEETKPEIAERRPMQWQSTRKPECVFQNPAAFAPLRHVPDVTLKADKESPPISVPKWLDP KSACETPSSSSLDDYMKCFKTPVVKNDFPPACPSSTPYSQLARLQQQQQQGLSTPLQSLQISGSSSINEC ISVNGRIYSILKQIDSGGSSKVFQVLNEKKQINAIKYVNLEDADSQTIESYRNEIAFLNKLQQHSDKIIR LYDYEITEQYIYMVMECGNIDLNSWLKKKKSINPWERKSYWKNMLEAVHIIHQHGIVHSDLKPANFVIVD GMLKLIDFGIANQMQPDTTSIVKDSQVGTVNYMAPEAIRDMSSSRENSKIRTKVSPRSDVWSLGCILYYM TYGRTPFQHIINQVSKLHAIINPAHEIEFPEISEKDLRDVLKCCLVRNPKERISIPELLTHPYVQIQPHP GSQMARGATDEMKYVLGQLVGLNSPNSILKTAKTLYERYNCGEGQDSSSSKTFDKKRERK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC058851
ORF Size	2492 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058851, AAH58851
RefSeq Size	2906 bp
RefSeq ORF	2492 bp
Locus ID	22137

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