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Vav2 (BC053060) Mouse Tagged ORF Clone
CAT＃: MG210853
- TrueORF®
Vav2 (tGFP-tagged) - Mouse vav 2 oncogene (cDNA clone MGC:62363 IMAGE:5704852)
ORF Plasmid: DDK
"BC053060" in other vectors (3)
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CNY 5,230.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2810040F13Rik; AI847175; Vav-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210853 representing BC053060
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAGTGGCGGCAATGCGGCCGCTGGCTCATCGACTGCAAGGTCCTGCCGCCCAACCACCGCGTCG TGTGGCCCTCGGCGGTGGTCTTCGACCTGGCGCAGGCGCTGCGCGACGGCGTCCTTCTGTGCCAGCTGCT GCACAACCTCTCCCCCGGTTCCATCGACCTTAAGGACATCAACTTCCGGCCGCAGATGTCCCAGGTCATC TCTGCAGTGTCCCGTCTGTCCCTGCACAGCATCGCACAGAGCAAAGGGATCAGGCCATTTCCATCAGAGG AGACAGCTGAAAATGATGACGACGTCTACCGGAGCCTGGAAGAGCTGGCTGACGAGCATGACCTGGGTGA GGACATCTACGACTGTGTCCCGTGTGAAGATGAAGGAGATGACATTTATGAGGACATCATCAAGGTGGAG GTGCAGCAGCCCATGATCAGATACATGCAGAAAATGGGCATGACTGAGGACGACAAGAGAAGCTGCTGCT TGTTAGAGATTCAGGAGACCGAGGCCAAGTACTACCGCACCCTGGAGGACATTGAGAAGAACTACATGGG TCCCTTGCGGCTGGTGCTGAGCCCGGCGGATATGGCTGCTGTCTTCATCAACCTGGAGGACCTCATCAAG GTGCATCACAGCTTTCTGCGAGCCATCGATGTGTCCATGATGGCTGGTGGCAGTACCCTGGCTAAGGTCT TTCTGGAGTTTAAGGAAAGGCTCCTGATCTATGGAGAGTACTGTAGCCACATGGAACACGCTCAGAGTAC ACTGAACCAGCTCCTCGCCAGCCGAGAGGACTTCAGGCAGAAAGTGGAGGAGTGCACACTCAGGGTTCAG GATGGCAAGTTCAAGCTGCAAGACCTGCTGGTGGTGCCCATGCAACGGGTGCTGAAGTACCACCTGCTGC TCAAGGAGCTCCTGAGCCATTCTGCAGACCGACCAGAAAGACAACAGCTCAAAGAAGCCCTGGAAGCCAT GCAGGACTTGGCCATGTACATCAATGAAGTGAAGCGGGACAAGGAGACCTTGAAGAAGATTAGCGAGTTC CAGTGCTCCATAGAAAACCTGCAAGTGAAGCTGGAGGAATTTGGGAGGCCAAAGATTGACGGGGAGCTTA AAGTCCGGTCCATAGTCAACCACACCAAGCAAGACAGGTACCTGTTCCTATTTGACAAGGTGGTCATCGT GTGCAAGAGGAAGGGCTACAGCTATGAGCTGAAGGAGGTCATTGAGCTGCTCTTCCACAAGATGACCGAT GACCCGATGCACAACAAGGACATCAAGAAGTCTCATGGGAAGATGTGGTCCTATGGCTTCTACCTGATTC ACCTCCAAGGAAAGCAAGGCTTTCAGTTCTTCTGCAAGACGGAAGACATGAAGCGGAAGTGGATGGAGCA GTTCGAGATGGCCATGTCAAACATCAAGCCAGATAAGGCCAATGCCAACCATCATAGCTTCCAGATGTAC ACATTCGACAAGACTACCAACTGCAAAGCCTGCAAGATGTTTCTCAGGGGTACCTTCTACCAGGGATACC TGTGTACCAGATGTGGCGTCGGGGCACACAAGGAATGCCTGGAGGTGATCCCCCCCTGCAAGATGAGTTC ACCTGCAGATGTGGATGCTCCTGGAGCAGGACCAGGTCCCAAGATGGTTGCTGTGCAGAATTACCATGGT AACCCAGCCCCTCCTGGGAAGCCTGTGTTGACCTTCCAGACAGGAGACGTGATAGAACTGCTCCGGGGTG ACCCCGATTCTCCTTGGTGGGAAGGGCGGCTGGTTCAAACCAGGAAGTCAGGGTATTTTCCCAGCTCATC TGTGAAGCCCTGCCCAGTGGATGGAAGGCCGCCTACTGGCCGCCCACCATCCCGGGAGATCGATTACACT GCCTACCCGTGGTTTGCTGGCAACATGGAACGGCAGCAGACGGACAATCTGCTCAAGTCTCATGCCAGTG GGACCTACCTCATCAGAGAGCGTCCAGCAGAGGCAGAACGCTTCGCCATCAGCATCAAGTTCAATGATGA GGTGAAACATATCAAGGTGGTGGAGAAAGATAGCTGGATCCACATCACGGAAGCTAAGAAGTTTGAGAGC CTCCTGAGCATTCTTTCAGCAATATTTGGGAGCCCTGCGCCTGAGGGAGAGGTTTTTGAGGCACTGGCCA TCCTAGCTGGGCTTGGCTGGAGAGCTGGTGGAGTACTACCAGTGTCACTCACTCAAGGAAAGCTTCAAGC AGTTGGACACTACACTCAAGTTCCCCTACAAGTCTCGGGAGCGAACCACCAGCAGGGCCTCAAGCCGGTC TCCAGCTTCCTGCGCTTCCTACAACTTTTCTTTTCTCAGTCCTCAGGGCCTCAGCTTTGCCCCCCAGGCC CCTTCTGCTCCCTTCTGGTCAGTGTTCACACCGCGGGTCATCGGCACAGCCGTGGCCAGGTACAACTTTG CTGCTCGAGACATGCGGGAGCTGTCACTGCGAGAAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210853 representing BC053060
Red=Cloning site Green=Tags(s) MEQWRQCGRWLIDCKVLPPNHRVVWPSAVVFDLAQALRDGVLLCQLLHNLSPGSIDLKDINFRPQMSQVI SAVSRLSLHSIAQSKGIRPFPSEETAENDDDVYRSLEELADEHDLGEDIYDCVPCEDEGDDIYEDIIKVE VQQPMIRYMQKMGMTEDDKRSCCLLEIQETEAKYYRTLEDIEKNYMGPLRLVLSPADMAAVFINLEDLIK VHHSFLRAIDVSMMAGGSTLAKVFLEFKERLLIYGEYCSHMEHAQSTLNQLLASREDFRQKVEECTLRVQ DGKFKLQDLLVVPMQRVLKYHLLLKELLSHSADRPERQQLKEALEAMQDLAMYINEVKRDKETLKKISEF QCSIENLQVKLEEFGRPKIDGELKVRSIVNHTKQDRYLFLFDKVVIVCKRKGYSYELKEVIELLFHKMTD DPMHNKDIKKSHGKMWSYGFYLIHLQGKQGFQFFCKTEDMKRKWMEQFEMAMSNIKPDKANANHHSFQMY TFDKTTNCKACKMFLRGTFYQGYLCTRCGVGAHKECLEVIPPCKMSSPADVDAPGAGPGPKMVAVQNYHG NPAPPGKPVLTFQTGDVIELLRGDPDSPWWEGRLVQTRKSGYFPSSSVKPCPVDGRPPTGRPPSREIDYT AYPWFAGNMERQQTDNLLKSHASGTYLIRERPAEAERFAISIKFNDEVKHIKVVEKDSWIHITEAKKFES LLSILSAIFGSPAPEGEVFEALAILAGLGWRAGGVLPVSLTQGKLQAVGHYTQVPLQVSGANHQQGLKPV SSFLRFLQLFFSQSSGPQLCPPGPFCSLLVSVHTAGHRHSRGQVQLCCSRHAGAVTARR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC053060 |
| ORF Size | 2489 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC053060, AAH53060 |
| RefSeq Size | 5113 bp |
| RefSeq ORF | 2489 bp |
| Locus ID | 22325 |
| Gene Summary | This gene encodes a member of the Vav family of Rho guanine nucleotide exchange factors. Vav family proteins are involved in the development and activation of lymphocytes, and the encoded protein may also be involved in angiogenesis. Disruption of this gene in mice is associated with heart, artery, and kidney defects, as well as tachycardia and hypertension. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220695 | Vav2 (untagged) - Mouse vav 2 oncogene (cDNA clone MGC:62363 IMAGE:5704852), (10ug) |
CNY 9,120.00 |
|
| MR210853 | Vav2 (Myc-DDK-tagged) - Mouse vav 2 oncogene (cDNA clone MGC:62363 IMAGE:5704852) |
CNY 6,072.00 |
|
| MR210853L3 | Lenti ORF clone of Vav2 (Myc-DDK-tagged) - Mouse vav 2 oncogene (cDNA clone MGC:62363 IMAGE:5704852) |
CNY 6,650.00 |
