Scyl1 (BC034519) Mouse Tagged ORF Clone
CAT#: MG210762
- TrueORF®
Scyl1 (tGFP-tagged) - Mouse SCY1-like 1 (S. cerevisiae) (cDNA clone MGC:18927 IMAGE:3672524)
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CNY 6,370.00
货期*
4周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2810011O19Rik; C85140; mdf; mfd; Ntkl; p105 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210762 representing BC034519
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGTTCTTTGCCCGGGACCCGGTCCGGGACTTCCCGTTCGAGCTGAGCCCTGAGCCCCCCGAAGGCG GGCCGCCCGGGCCCTGGATCCTGCACCGAGGCCGCAAAAAGGCCACAGGCAGCGCAGTGTCCATCTTCGT GTATGATGTGAAACCGGGAGCTGAAGAGCAGACCCAGGTGGCCAAAGCTGCCTTCAAACGCCTCAAAACT CTCCGACACCCCAACATCCTGGCCTATATCGATGGGTTGGAGACAGAAAAGTGCCTCCACATCGTGACAG AGGCTGTGACCCCCCTGGGAACATACCTCAAGGCACGAGCAGAAGCAGGTGGCCTGAAGGAGCAGGAGCT GTCATGGGGGTTACACCAGATCGTGAAAGCCCTCAGCTTCCTGGTCAACGACTGCAACCTCATCCACAAT AATGTCTGCATGGCCGCTGTGTTTGTGGACAGGGCTGGCGAGTGGAAACTTGGGGGTCTGGACTACATGT ACTCGGCACAGGGCAACGGCGGGGGACCACCCAGCAAGGGGATCCCGGAGCTCGAGCAGTATGATCCCCC GGAGCTGGCTGACAGCAGTAGCAGAGCAGTCAGAGAGAAGTGGTCAGCAGACATGTGGCGCTTGGGCTGC CTCATCTGGGAAGTTTTCAATGGGTCTCTACCTCGGGCAGCTGCCCTGCGCAACCCTGGGAAGATCCCCA AATCCCTGGTGACCCATTACTGTGAACTGGTGGGAGCTAACCCAAAAGTACGTCCCAACCCGGCCCGCTT CCTGCAGAACTGCCGGGCACCCGGTGGCTTCATGAGCAACCGCTTTGTTGAGACCAACCTCTTCCTGGAG GAGATTCAGATCAAAGAGCCAGCTGAGAAGCAGAAGTTCTTCCAAGAGCTGAGCAAGAGTCTAGACTCAT TTCCCGAAGATTTCTGTCGACACAAGGTGCTGCCCCAGCTACTGACTGCCTTTGAGTTTGGCAATGCTGG GGCCGTGGTCCTCACACCTCTCTTCAAGGTGGGAAAATCCCTCCGTGCTGAAGAGTACCAGGAGAAGATC ATCCCTGTGGTAGTTAAGATGTTCTCATCCACCGACCGGGCCATGCGCATCCGCCTCCTCCAGCAGATGG AGCAGTTCATCCAATACCTTGATGAGCCAACAGTCAACACGCAGATTTTCCCCCACGTCACACATGGCTT CCTGGACACCAACCCCGCCATCCGCGAGCAGACGGTCAAGTCCATGCTGCTCTTGGCCCCAAAGCTGAAT GAGGCCAATCTCAATGTGGAACTGATGAAGCACTTTGCAAGGCTACAAGCCAAGGACGACCAGGGTCCTA TCCGCTGCAACACCACGGTCTGCTTGGGCAAAATCGGCTCCTATCTCAGTGCTAGTACTAGACACAGGGT CCTCACCTCCGCCTTCAGCAGAGCCACTAAGGACCCATTTGCACCATCCCGGGTTGCGGGTGTCCTGGGC TTTGCTGCCACACACAATCTCTATTCGATGGACGACTGTGCCCATAAGATCCTGCCTGTGCTCTGTGGCC TTACTGTGGACCCTGAGAAATCTGTGCGGGACCAGGCCTTTAAGACCATTCGAAGCTTCCTGTCCAAATT AGAGTCTGTGTCAGAGGATCCCACCCAGCTGGCAGAAGTAGAGAAGGATGTCCATGCAGCGTCCAGTCCT GGAACAGGAGGAGCTGCAGCCAGCTGGGCAGGCTGGGCTGTGACTGGGGTATCCTCTCTCACCTCCAAGC TGATCCGAGCACACCCCACGCCTGTGCCGTCTGATACCACTGTGCCCCAGAGACCAGTGCCAGAGGGAAA TCCTGCTCCAGCCCCTGCCCTTGCCCAAGCTATCCCTGCAACCTCAGGGCACTGGGAGACACAGGAAGAC AAGGACACTGCAGAAGACAGCGCCACTGCTGACAGATGGGACGATGAGGACTGGGGCAGCTTGGAGCAGG AAGCTGAATCCGTGTTGGCACAGCAGGATGACTGGAGTGCCAAGGGCCAAGGAAGCCGAGCTGGACAGGT CAACCACCCAGACCACAAATCTCTGGAATCACATTGGAGCAGCTGGGAAGTTGAGGGCTCCTGGGACCAG GGCTGGCAGGAACCCAGCTCTGTGGAGCCACCTCCAGAAGGCACTCGGCTAGCTAGCGAATATAACTGGG GTGGTGCAGAGCCCAGTGACAAGGGCGACCCCTTTGCTGCCCTGTCTGTTCGTCCCAGCGCTCAGCCCAG GCCAGACCCAGACTCCTGGGGTGAAGACAACTGGGAAGGCTTGGAGGCTGAGAGCAGACAGGTAAAGGCA GAGCTGGCCCGGAAAAAGCGAGAGGAAAGGAGAAGAGAAATGGAAGCCAAACGGGCAGAGAAAAAGACCA CCAAGGGGCCCATGAAGCTGGGAGCCCGGAAGCTGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210762 representing BC034519
Red=Cloning site Green=Tags(s) MWFFARDPVRDFPFELSPEPPEGGPPGPWILHRGRKKATGSAVSIFVYDVKPGAEEQTQVAKAAFKRLKT LRHPNILAYIDGLETEKCLHIVTEAVTPLGTYLKARAEAGGLKEQELSWGLHQIVKALSFLVNDCNLIHN NVCMAAVFVDRAGEWKLGGLDYMYSAQGNGGGPPSKGIPELEQYDPPELADSSSRAVREKWSADMWRLGC LIWEVFNGSLPRAAALRNPGKIPKSLVTHYCELVGANPKVRPNPARFLQNCRAPGGFMSNRFVETNLFLE EIQIKEPAEKQKFFQELSKSLDSFPEDFCRHKVLPQLLTAFEFGNAGAVVLTPLFKVGKSLRAEEYQEKI IPVVVKMFSSTDRAMRIRLLQQMEQFIQYLDEPTVNTQIFPHVTHGFLDTNPAIREQTVKSMLLLAPKLN EANLNVELMKHFARLQAKDDQGPIRCNTTVCLGKIGSYLSASTRHRVLTSAFSRATKDPFAPSRVAGVLG FAATHNLYSMDDCAHKILPVLCGLTVDPEKSVRDQAFKTIRSFLSKLESVSEDPTQLAEVEKDVHAASSP GTGGAAASWAGWAVTGVSSLTSKLIRAHPTPVPSDTTVPQRPVPEGNPAPAPALAQAIPATSGHWETQED KDTAEDSATADRWDDEDWGSLEQEAESVLAQQDDWSAKGQGSRAGQVNHPDHKSLESHWSSWEVEGSWDQ GWQEPSSVEPPPEGTRLASEYNWGGAEPSDKGDPFAALSVRPSAQPRPDPDSWGEDNWEGLEAESRQVKA ELARKKREERRREMEAKRAEKKTTKGPMKLGARKLD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC034519 |
ORF Size | 2420 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC034519, AAH34519 |
RefSeq Size | 2648 bp |
RefSeq ORF | 2420 bp |
Locus ID | 78891 |
Gene Summary | Regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum. Involved in the maintenance of the Golgi apparatus morphology. Has no detectable kinase activity in vitro.[UniProtKB/Swiss-Prot Function] |
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