Smyd4 (BC095952) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	G430029E23Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210723 representing BC095952 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTACCGGTGGATGAATGGAAGTCCTACCTACTTAAGAAGTGGGCTTCACTCCCGAAGTCTGTGC AGGACACAATTTCTACAGCAGAGACTTTGAGCGACATCTTCCTTCCTTCTTCTTCCCTTCTTCAACCTGA GGATGAGATGTTTTTGAAGGAACTTTCTAGTAGTTACTCGGTGGAAAAGGACTATGATGCTCCCCTCTTC TACAGAGAAGAAGGAAACAGGAAATTCCAAGAAAAGGAGTACACAGATGCTGCAGTGCTGTACTCTAAGG GAGTGTCTCATTCAAGGCCTAACACTGAGGACATTTCACTGTGCTATGCCAATCGCTCTGCAGCGCTCTT CCATCTGGGTCAGTATGAAGCATGTCTTAAAGACATAGTGGAAGCAGGTATGCATGGGTATCCTGAAAGA CTGCAGCCCAAGATGATGGTGCGTAAGACAGAATGCCTGGTGAACCTGGGGAGACTCCAGGAGGCAAGAC AGACCATCAGTGATCTCGAAAGCAGCCTCACTGCCAAGCCAACCCTGGTGCTTTCCTCTTACCAGATTCT GCAAAGGAATGTCCAGCATCTGAAAATAAAGATCCAAGAAAAGGAGACTCTCCCAGAACCCGTCCCTGCA GCTCTCACCAATGCCTTTGAGGATATAGCCCTGGGGGAAGAGAACAAACAGATTTCTGGGGCCTCCCTCT CTGTCAGCTTATGCACACACCCTTTGAAAGGCCGCCATCTAGTTGCCACAAAAGACATTCTCCCAGGAGA ACTGCTGGTGAAGGAAGATGCTTTTGTAAGTGTCCTTATCCCAGGAGAAATGCCACGACCTCATCATTGC CTTGAGAACAAGTGGGATACCAGAGTTACCAGTGGAGACCTCTACTGTCACCGATGTCTGAAGCACACTT TGGCCACAGTACCTTGTGGCAGCTGCAGCTATGCCAAGTATTGCAGCCAGGAATGTATGCAGCAGGCATG GGACCTCTACCATAGCACAGAGTGTTCTCTTGGGGGGCTGCTCCTCACACTCGGGGTCTTCTGCCATGTT GCCCTGAGAATGACTCTTTTAGCCAGATTTGAAGATGTTGATAGAGTTGTAAGGATGCTTTGTGACGAGG TTGGTAGCACAGACACCTGTTTACCTGAAAGCAAGAATCTGGTCAAGGCATTTGATTACACAAGTCAGGA AGAGAGTGAAGAGAAGAGCAAGATAGGTGAACCCCCAATTCCTGGATGCAATGTCAATGGAAAGTATGGA AGTAATTATAATGCTATCTTCAGCCTTTTGCCCCATACTGAAAAGCATAGCCCAGAACACAGATTCATCT GTGCCATCAGTGTCTCCGCACTGTGCAGACAACTCAAAGCCGCCAGCGTGCAGGCCCAAACCTTAAAGTC CCCTAAGCTGAAAGCAGTGACCCCAGGGCTGTGTGCAGATTTGACTGTTTGGGGAACAGCCATGCTGCGA CACATGCTACAGCTGCAGTGTAATGCCCAGGCAATAACATCCATATGTCACACAGGGTCTAACGAGAGCA TCATTACCAACAGCAGGCAGATACGCCTTGCCACAGGCGTCTTCCCTGTTGTCAGCCTCCTGAACCACTC CTGCAGGCCCAACACCAGTGTGTCCTTCACTGGCACTGTCGCCACCATCCGGGCAGCACAGAGGATCGCA AAAGGACAGGAGATTCTGCACTGCTATGGGCCTCACGAGAGCCGGATGGGCGTTGCTGAGAGGCAGCAGA GGCTGAGTTCTCAGTACTTCTTTGACTGCCGCTGTGGGGCCTGTCACGCTGAGACACTGAGAGCAGCTGC AGCTCCCAGATGGGAAGCCTTCTGTTGTAAGACTTGCAGAGCGCTCATGCAGGGAAATGATGTGCTGAGC TGTAGCAACGAATCTTGCACAAACTCTGTCAGCAGGGACCAGCTAGTCTCTCGCCTGCAGGACCTTCAGC AACAGGTCTGCATGGCCCAGAAGCTTCTCCGAACCGGTAAACCAGAGCAAGCCATTCAGCAGTTGTTGCG GTGCCGGGAGGCTGCTGAGAGCTTCTTGTCAGCAGAGCACACCGTACTGGGGGAAATTGAAGACGGCCTG GCCCAGGCCCATGCTACCTTAGGGAACTGGCTAAAGTCTGCTGCCCATGTACAGAAGAGTCTCCAGGTGG TTGAAACTCGTCATGGGCCATCCAGTGTTGAAATTGGCCATGAGCTCTTCAAACTGGCCCAAGTCCTGTT CAATGGGTTGGCAGTGCCTGAAGCTCTGAGTGCCATCTGGAAGGCAGAAAGGATCCTGTTGGTGCACTGT GGCCCTGAGAGTGAGGAGGTCCGGGAGCTCCGGGAAATGAGGTCCTGCTTACTGGACTCGTCATTCGTCC CTGTGGGGCCCTTGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210723 representing BC095952 Red=Cloning site Green=Tags(s) MDLPVDEWKSYLLKKWASLPKSVQDTISTAETLSDIFLPSSSLLQPEDEMFLKELSSSYSVEKDYDAPLF YREEGNRKFQEKEYTDAAVLYSKGVSHSRPNTEDISLCYANRSAALFHLGQYEACLKDIVEAGMHGYPER LQPKMMVRKTECLVNLGRLQEARQTISDLESSLTAKPTLVLSSYQILQRNVQHLKIKIQEKETLPEPVPA ALTNAFEDIALGEENKQISGASLSVSLCTHPLKGRHLVATKDILPGELLVKEDAFVSVLIPGEMPRPHHC LENKWDTRVTSGDLYCHRCLKHTLATVPCGSCSYAKYCSQECMQQAWDLYHSTECSLGGLLLTLGVFCHV ALRMTLLARFEDVDRVVRMLCDEVGSTDTCLPESKNLVKAFDYTSQEESEEKSKIGEPPIPGCNVNGKYG SNYNAIFSLLPHTEKHSPEHRFICAISVSALCRQLKAASVQAQTLKSPKLKAVTPGLCADLTVWGTAMLR HMLQLQCNAQAITSICHTGSNESIITNSRQIRLATGVFPVVSLLNHSCRPNTSVSFTGTVATIRAAQRIA KGQEILHCYGPHESRMGVAERQQRLSSQYFFDCRCGACHAETLRAAAAPRWEAFCCKTCRALMQGNDVLS CSNESCTNSVSRDQLVSRLQDLQQQVCMAQKLLRTGKPEQAIQQLLRCREAAESFLSAEHTVLGEIEDGL AQAHATLGNWLKSAAHVQKSLQVVETRHGPSSVEIGHELFKLAQVLFNGLAVPEALSAIWKAERILLVHC GPESEEVRELREMRSCLLDSSFVPVGPLV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC095952
ORF Size	2397 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC095952, AAH95952
RefSeq Size	3037 bp
RefSeq ORF	2399 bp
Locus ID	319822

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.