Ptpra (BC050930) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	PTP[a], RPTR[a], PTPalpha, RPTRalpha
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210691 representing BC050930 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCCTGGTTCATTCTTGTCCTGTTTGGCAGTGGTCTAATACATGTTAGTGCCAACAATGCTACTA CAGTTTCACCTTCTTTAGGAACGACAAGATTAATTAAAACATCAACAACAGAATTGGCTAAGGAAGAGAA TAAAACCTCAAATTCAACCTCTTCAGTAATTTCTCTTTCTGTGGCACCAACATTCAGCCCAAACCTGACT CTGGAGCCCACCTATGTGACTACTGTTAATTCTTCACACTCTGACAATGGGACCAGGAGGGCAGCCAGCA CGGAATCTGGAGGCACTACCATTTCCCCGAACGGAAGCTGGCTTATTGAGAACCAGTTCACGGATGCCAT AACAGAACCCTGGGAGGGGAACTCCAGCACTGCAGCAACCACTCCAGAAACCTTCCCCCCGGCAGATGAG ACACCAATTATTGCGGTGATGGTGGCCCTGTCCTCTCTGCTAGTAATCGTGTTTATTATCATAGTTCTGT ACATGTTAAGGTTTAAGAAATACAAGCAAGCTGGTAGTCATTCCAACTCTTTCCGCCTGTCAAATGGCCG CACGGAGGATGTGGAGCCCCAAAGTGTACCACTTCTGGCCAGGTCCCCAAGCACCAACAGGAAGTACCCA CCACTGCCTGTGGACAAGCTGGAAGAGGAGATTAACCGGAGAATGGCTGATGACAATAAGCTCTTCAGAG AAGAATTCAACGCTCTCCCTGCTTGTCCTATCCAGGCCACCTGTGAGGCTGCCTCCAAGGAAGAAAACAA GGAAAAAAACCGCTATGTAAACATCCTGCCCTATGACCACTCTAGAGTGCACCTGACACCTGTTGAAGGG GTCCCAGATTCTGATTACATCAACGCTTCATTCATTAATGGCTACCAGGAAAAGAACAAATTCATCGCTG CACAAGGACCAAAAGAAGAAACAGTGAATGACTTCTGGAGAATGATATGGGAACAAAACACAGCTACTAT TGTCATGGTGACCAACCTGAAGGAGAGAAAGGAGTGTAAATGTGCCCAATACTGGCCAGACCAAGGCTGC TGGACCTATGGGAATGTCCGTGTGTCTGTCGAGGATGTGACTGTTCTGGTGGACTACACAGTACGGAAAT TCTGCATCCAGCAGGTGGGCGACGTGACCAACAGGAAACCACAGCGCCTCATCACTCAGTTCCACTTCAC CAGCTGGCCAGACTTTGGGGTGCCTTTCACCCCAATTGGCATGCTCAAGTTCCTCAAGAAGGTGAAGGCC TGTAACCCTCAGTACGCAGGGGCTATCGTGGTCCACTGCAGTGCAGGTGTAGGGCGCACTGGCACCTTTG TTGTCATCGATGCCATGCTGGACATGATGCATTCGGAACGCAAAGTGGATGTATACGGGTTTGTGAGCCG GATCCGGGCCCAGCGCTGCCAGATGGTACAGACAGACATGCAGTACGTCTTCATATACCAGGCCCTTCTG GAGCATTATCTGTATGGGGACACAGAACTGGAAGTGACTTCTCTAGAAACCCACCTACAAAAAATTTATA ACAAGATCCCAGGGACGAGCAACAACGGGTTAGAGGAGGAGTTTAAGAAATTAACTTCAATCAAAATCCA GAATGACAAGATGCGCACGGGAAACCTTCCAGCCAACATGAAGAAGAACCGGGTTTTACAGATCATTCCA TATGAATTTAACAGAGTGATCATTCCAGTCAAACGAGGCGAAGAGAACACAGACTATGTGAACGCATCCT TCATTGATGGATACCGGCAGAAAGACTCCTACATTGCCAGCCAGGGCCCTCTTCTCCACACGATTGAGGA CTTCTGGCGAATGATCTGGGAGTGGAAGTCCTGTTCTATCGTAATGCTGACAGAACTGGAAGAGAGAGGC CAGGAGAAGTGTGCCCAGTACTGGCCATCTGATGGCCTGGTGTCCTATGGAGACATCACAGTTGAGCTGA AGAAGGAGGAGGAATGTGAAAGCTACACTGTCCGAGACCTCCTGGTCACCAACACCAGGGAGAACAAGAG TCGGCAAATCCGGCAGTTCCACTTCCACGGCTGGCCTGAGGTGGGCATCCCCAGCGACGGCAAGGGCATG ATCAACATCATTGCAGCAGTGCAGAAGCAGCAGCAGCAGTCGGGGAACCATCCCATCACTGTGCACTGCA GTGCCGGGGCAGGACGGACAGGAACCTTCTGTGCCTTGAGCACAGTCCTGGAACGTGTGAAAGCAGAAGG AATTTTAGATGTCTTCCAAACTGTCAAGAGCCTGCGGCTGCAGAGGCCACACATGGTCCAGACACTGGAA CAGTATGAATTCTGCTACAAGGTGGTACAGGAGTACATTGACGCCTTTTCAGATTATGCCAACTTCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210691 representing BC050930 Red=Cloning site Green=Tags(s) MDSWFILVLFGSGLIHVSANNATTVSPSLGTTRLIKTSTTELAKEENKTSNSTSSVISLSVAPTFSPNLT LEPTYVTTVNSSHSDNGTRRAASTESGGTTISPNGSWLIENQFTDAITEPWEGNSSTAATTPETFPPADE TPIIAVMVALSSLLVIVFIIIVLYMLRFKKYKQAGSHSNSFRLSNGRTEDVEPQSVPLLARSPSTNRKYP PLPVDKLEEEINRRMADDNKLFREEFNALPACPIQATCEAASKEENKEKNRYVNILPYDHSRVHLTPVEG VPDSDYINASFINGYQEKNKFIAAQGPKEETVNDFWRMIWEQNTATIVMVTNLKERKECKCAQYWPDQGC WTYGNVRVSVEDVTVLVDYTVRKFCIQQVGDVTNRKPQRLITQFHFTSWPDFGVPFTPIGMLKFLKKVKA CNPQYAGAIVVHCSAGVGRTGTFVVIDAMLDMMHSERKVDVYGFVSRIRAQRCQMVQTDMQYVFIYQALL EHYLYGDTELEVTSLETHLQKIYNKIPGTSNNGLEEEFKKLTSIKIQNDKMRTGNLPANMKKNRVLQIIP YEFNRVIIPVKRGEENTDYVNASFIDGYRQKDSYIASQGPLLHTIEDFWRMIWEWKSCSIVMLTELEERG QEKCAQYWPSDGLVSYGDITVELKKEEECESYTVRDLLVTNTRENKSRQIRQFHFHGWPEVGIPSDGKGM INIIAAVQKQQQQSGNHPITVHCSAGAGRTGTFCALSTVLERVKAEGILDVFQTVKSLRLQRPHMVQTLE QYEFCYKVVQEYIDAFSDYANFK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC050930
ORF Size	2381 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC050930, AAH50930
RefSeq Size	2917 bp
RefSeq ORF	2381 bp
Locus ID	19262
Gene Summary	Tyrosine protein phosphatase which is involved in integrin-mediated focal adhesion formation (PubMed:22801373). Following integrin engagement, specifically recruits BCAR3, BCAR1 and CRK to focal adhesions thereby promoting SRC-mediated phosphorylation of BRAC1 and the subsequent activation of PAK and small GTPase RAC1 and CDC42 (PubMed:22801373).[UniProtKB/Swiss-Prot Function]

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