Clcn3 (BC057133) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Clc3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210671 representing BC057133 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCTTCTTCTGATCCCTATTTGCCTTATGACGGGGGAGGAGACAGTATTCCCTTGAGAGAATTAC ATAAAAGAGGAACTCATTATACAATGACAAATGGAGGCAGCATTAATAGCTCTACACACTTGCTGGATCT TTTGGATGAGCCTATCCCAGGTGTCGGTACCTACGATGATTTCCATACTATTGACTGGGTGCGAGAGAAG TGTAAGGACAGAGAAAGGCACAGACGGATCAACAGTAAAAAAAAAGAATCAGCATGGGAAATGACAAAAA GTCTGTATGACGCCTGGTCAGGATGGCTTGTTGTTACACTGACGGGACTGGCATCAGGGGCACTAGCTGG ATTGATAGACATTGCTGCTGACTGGATGACTGACCTGAAGGAGGGCATCTGCCTCAGTGCATTGTGGTAC AACCATGAACAGTGTTGTTGGGGCTCTAATGAGACAACGTTTGAAGAGAGGGATAAATGTCCACAGTGGA AAACATGGGCAGAGTTAATCATTGGCCAAGCAGAGGGCCCTGGATTTTATATCATGAACTACATCATGTA CATCTTTTGGGCTTTGAGTTTTGCCTTTCTTGCAGTTTCTTTGGTGAAAGTATTTGCTCCATATGCCTGT GGCTCTGGAATTCCAGAGATTAAAACTATTTTGAGTGGATTTATCATCAGAGGATACTTGGGAAAATGGA CTTTAATGATTAAAACTATCACGTTAGTGCTGGCTGTGGCATCAGGTTTGAGTTTAGGAAAAGAAGGTCC CCTGGTACATGTTGCCTGCTGCTGTGGAAACATCTTTTCCTACCTCTTTCCAAAGTATAGCACCAATGAA GCTAAAAAGAGGGAGGTGCTGTCAGCCGCCTCAGCTGCTGGGGTTTCTGTGGCTTTTGGTGCACCGATCG GAGGAGTTCTTTTTAGCTTGGAGGAGGTTAGCTATTATTTTCCTCTCAAAACTTTATGGAGATCATTTTT TGCTGCTTTGGTGGCAGCATTTGTTTTGAGATCCATCAATCCATTTGGTAACAGCCGTCTGGTCCTCTTT TATGTGGAGTATCATACACCATGGTACCTTTTTGAACTGTTTCCTTTTATTCTCCTAGGGGTATTTGGAG GGCTTTGGGGAGCTTTTTTTATTAGGGCAAATATTGCCTGGTGTCGTCGACGCAAGTCCACCAAATTTGG AAAGTATCCTGTTCTCGAAGTCATTATTGTTGCAGCCATTACTGCTGTGATAGCCTTCCCCAACCCATAC ACAAGGCTCAACACCAGTGAACTGATTAAAGAGCTGTTTACAGATTGTGGGCCGTTGGAATCCTCCTCTC TCTGTGACTACAGAAATGACATGAATGCCAGTAAAATTGTTGACGATATTCCTGACCGTCCAGCAGGCGT TGGAGTATATTCAGCTATCTGGCAGTTGTGCCTAGCGCTCATATTTAAAATAATAATGACAGTATTCACT TTTGGTATCAAGGTCCCGTCAGGCTTGTTTATCCCCAGCATGGCCATTGGAGCCATTGCAGGGAGAATTG TGGGGATCGCTGTGGAGCAGCTTGCCTACTATCACCACGACTGGTTTATCTTCAAGGAGTGGTGTGAGGT TGGGGCTGACTGCATCACTCCCGGGCTGTATGCCATGGTTGGGGCTGCTGCGTGCTTAGGTGGTGTGACA AGAATGACTGTGTCTCTGGTGGTTATTGTTTTTGAACTTACTGGAGGCTTGGAATATATTGTTCCTCTTA TGGCTGCAGTAATGACCAGTAAATGGGTTGGTGATGCCTTTGGTAGGGAAGGTATTTATGAAGCACACAT CCGACTAAATGGGTACCCTTTCTTGGATGCAAAAGAAGAATTCACTCATACAACCCTGGCTGCTGATGTT ATGAGACCTCGAAGAAGTGACCCTCCCTTAGCTGTTTTGACACAGGACAATATGACAGTAGATGACATAG AAAACATGATTAATGAAACCAGCTATAATGGCTTTCCTGTTATAATGTCAAAAGAATCTCAGAGATTAGT GGGATTTGCCCTCAGAAGAGACCTGACTATTGCAATAGAAAGTGCCAGAAAAAAACAAGAAGGGATTGTT GGCAGTTCTCGGGTGTGTTTTGCACAGCATACTCCATCTCTTCCAGCAGAAAGTCCACGGCCATTAAAAC TGAGAAGCATCCTTGACATGAGCCCTTTTACAGTGACAGACCACACCCCAATGGAGATTGTGGTAGACAT CTTTCGAAAGCTTGGTCTGAGGCAGTGCCTTGTAACTCACAACGGACGCCTCCTTGGCATTATAACAAAA AAAGATATCCTCCGTCATATGGCCCAGACGGCAAACCAAGACCCCGCTTCAATAATGTTCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210671 representing BC057133 Red=Cloning site Green=Tags(s) MDASSDPYLPYDGGGDSIPLRELHKRGTHYTMTNGGSINSSTHLLDLLDEPIPGVGTYDDFHTIDWVREK CKDRERHRRINSKKKESAWEMTKSLYDAWSGWLVVTLTGLASGALAGLIDIAADWMTDLKEGICLSALWY NHEQCCWGSNETTFEERDKCPQWKTWAELIIGQAEGPGFYIMNYIMYIFWALSFAFLAVSLVKVFAPYAC GSGIPEIKTILSGFIIRGYLGKWTLMIKTITLVLAVASGLSLGKEGPLVHVACCCGNIFSYLFPKYSTNE AKKREVLSAASAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFVLRSINPFGNSRLVLF YVEYHTPWYLFELFPFILLGVFGGLWGAFFIRANIAWCRRRKSTKFGKYPVLEVIIVAAITAVIAFPNPY TRLNTSELIKELFTDCGPLESSSLCDYRNDMNASKIVDDIPDRPAGVGVYSAIWQLCLALIFKIIMTVFT FGIKVPSGLFIPSMAIGAIAGRIVGIAVEQLAYYHHDWFIFKEWCEVGADCITPGLYAMVGAAACLGGVT RMTVSLVVIVFELTGGLEYIVPLMAAVMTSKWVGDAFGREGIYEAHIRLNGYPFLDAKEEFTHTTLAADV MRPRRSDPPLAVLTQDNMTVDDIENMINETSYNGFPVIMSKESQRLVGFALRRDLTIAIESARKKQEGIV GSSRVCFAQHTPSLPAESPRPLKLRSILDMSPFTVTDHTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITK KDILRHMAQTANQDPASIMFN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC057133
ORF Size	2375 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC057133, AAH57133
RefSeq Size	4815 bp
RefSeq ORF	2375 bp
Locus ID	12725
Gene Summary	Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.[UniProtKB/Swiss-Prot Function]

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