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Dgkg (NM_138650) Mouse Tagged ORF Clone
CAT＃: MG210661
- TrueORF®
Dgkg (tGFP-tagged) - Mouse diacylglycerol kinase, gamma (Dgkg)
ORF Plasmid: DDK
"NM_138650" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 90kDa; 2900055E17Rik; AI854428; Dagk3; E430001K23Rik; mKIAA4131 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210661 representing NM_138650
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAAGAACAATGGGTCTCTCTCTCATCAGAAGAATTTGATCAACTCCAGAAATACTCTGAATATT CCTCCAAAAAGATAAAAGATGTATTGGCTGAGTTTAATGAAGGTGGAAGCCTCAGGCAGTATGACCCACA CAAGCCCATTAGCTATGATGTCTTCAAGCTGTTCATGAGGGCATACCTGGAGGTGGACCTTCCTCAGCCA CTGAGCACACACCTCTTCCTGGCCTTCAGCCAGAAGCCCAGACAAGAGACACCTGACCATCCAAAGGAGG GGGCCAGCAGTAGTGAGCCCAATGTCTCAGATTATAATTCGGATAATGCTGCCAAAGCAGATGAGGCCTG TGCTCCAGACACTGAATCAAAAACAACTAAGACACAAGCACCATCTAAAGAACTAGAGGCTGCAGCCCCA TGGGAAGACCCAGGTGCTCTGGCTTCAAGCTCAGATGCTCCCGTAGTGTACCTGAAGGATGTCGTGTGTT ACTTGTCCCTGATGGAGACCGGGCGACCTCAGGATAAGCTAGAATTCATGTTTCGTCTCTATGATTCGGA TGAAAATGGACTCCTGGACCAAGCGGAGATGGATCAGATTGTGAGCCAAATGCTGCACGTTGCCCAGTAC CTGGAATGGGATCCCACAGAGCTCAGACCTATACTGAAGGAGATGCTGCAAGGCATGGACTATGACAAGG ATGGCTTCGTGTCTCTACAGGAGTGGATCAACGGGGGAATGACCACCATCCCTTTGCTGGTCCTCCTGGG AATGGATGACTCTGGCTCCAAGGGCGATGGGAGGCATGCCTGGACCCTGAAGCACTTCAAGAAGCCCACT TACTGCAACTTCTGCCGCGCCATGCTGATGGGTGTTGGGAAGCAAGGCCTGTGCTGCATCTACTGTAAAT ATACAGTCCACCAACGCTGTGTATCCAAAACCATTCATGGATGTGTGAAAACAAACTCCAAAGCCAAAAG GAGTGGCGAGGTGATGCAGCATGCGTGGGTGGAAGGGAACTCCTCCGTCAAGTGTGACCGGTGCCACAAA AGTATCAAGTGCTACCAGAGTGTCACCGCGCGGCACTGCGTGTGGTGCCGGATGACGTTTCACCGCAAAT GTGAATTATCGACAGTGTGCGACGGTGGGGAACTCAAAGACCACATTTTGCTGCCTACCTCCATATGCCC CGTCTCCGGAGACCGGCAAGGAGGGAAGTCCGATGGCAGTGTGGCCGCCAAGGGTGAACTTGTAACTCAG TATAAGATCATCCCTAGCCCGGGGACGCATCCCCTGCTGGTCCTGGTGAACCCCAAGAGTGGAGGAAGAC AAGGAGAAAGAATTCTTCGGAAATTTCACTACCTGCTCAACCCCGAACAAGTTTTCAACCTGGACAATGG GGGACCTACACCAGGATTGAACTTCTTTCATGATACCCCAGACTTCCGTGTTCTTGCCTGTGGCGGAGAT GGGACCGTTGGCTGGATTTTGGATTGCATTGATAAGGCTAACTTCACAAAGCACCCACCGGTGGCTGTCC TGCCTCTTGGAACAGGGAATGACCTCGCACGATGCCTCCGCTGGGGAGGAGGTTACGAAGGGGGCAGCTT GACCAAAATCCTGAAGGAAATTGAACAGAGCCCCTTGGTGATGCTGGACCGTTGGTACCTAGAGGTCATG CCCAGGGAAGAGGTGGAGAATGGAGACCAAGTCCCATACAACATCATGAACAACTATTTCTCCATTGGTG TGGATGCTTCAATTGCACACAGATTCCATATGATGAGAGAGAAACACCCTGAAAAATTCAACAGCAGGAT GAAGAACAAGCTGTGGTATTTCGAATTTGGTACCTCGGAAACCTTTGCAGCCACCTGCAAGAAACTCCAT GACCACATAGAGCTGGAGTGTGACGGAGTTGAGGTGGATCTGAGCAACATCTTCCTGGAAGGCATCGCCA TTCTTAATATTCCCAGCATGTACGGAGGCACCAATCTCTGGGGAGAAACCAAGAAGAACCGGGCTGTGAT CCGGGAAAGCAGGAAGAGCGTCACAGACCCAAAGGAGCTGAAATGCTGTGTCCAAGACCTCAGCGATCAG CTCCTTGAAGTGGTGGGTCTAGAGGGAGCCATGGAGATGGGACAGATCTACACTGGCCTGAAGAGTGCGG GCAGGAGACTGGCCCAGTGCTCCTCTGTCACCATTAGGACTAACAAGCTTCTGCCGATGCAAGTAGACGG AGAGCCCTGGATGCAGCCACAATGCACGATTAAAATCACTCACAAAAACCAGGCACCTATGATGATGGGG CCTCCCCAGAAGAGCAGCTTTTTTTCTCTGAGGAGGAAAAGCCGTTCAAAAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210661 representing NM_138650
Red=Cloning site Green=Tags(s) MSEEQWVSLSSEEFDQLQKYSEYSSKKIKDVLAEFNEGGSLRQYDPHKPISYDVFKLFMRAYLEVDLPQP LSTHLFLAFSQKPRQETPDHPKEGASSSEPNVSDYNSDNAAKADEACAPDTESKTTKTQAPSKELEAAAP WEDPGALASSSDAPVVYLKDVVCYLSLMETGRPQDKLEFMFRLYDSDENGLLDQAEMDQIVSQMLHVAQY LEWDPTELRPILKEMLQGMDYDKDGFVSLQEWINGGMTTIPLLVLLGMDDSGSKGDGRHAWTLKHFKKPT YCNFCRAMLMGVGKQGLCCIYCKYTVHQRCVSKTIHGCVKTNSKAKRSGEVMQHAWVEGNSSVKCDRCHK SIKCYQSVTARHCVWCRMTFHRKCELSTVCDGGELKDHILLPTSICPVSGDRQGGKSDGSVAAKGELVTQ YKIIPSPGTHPLLVLVNPKSGGRQGERILRKFHYLLNPEQVFNLDNGGPTPGLNFFHDTPDFRVLACGGD GTVGWILDCIDKANFTKHPPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKEIEQSPLVMLDRWYLEVM PREEVENGDQVPYNIMNNYFSIGVDASIAHRFHMMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLH DHIELECDGVEVDLSNIFLEGIAILNIPSMYGGTNLWGETKKNRAVIRESRKSVTDPKELKCCVQDLSDQ LLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRTNKLLPMQVDGEPWMQPQCTIKITHKNQAPMMMG PPQKSSFFSLRRKSRSKD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138650 |
| ORF Size | 2364 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138650.2 |
| RefSeq Size | 3201 bp |
| RefSeq ORF | 2367 bp |
| Locus ID | 110197 |
| UniProt ID | Q91WG7 |
| Gene Summary | Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:32033984). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:32033984). Has no apparent specificity with regard to the acyl compositions of diacylglycerol (By similarity). Specifically expressed in the cerebellum where it controls the level of diacylglycerol which in turn regulates the activity of protein kinase C gamma (PubMed:32033984). Through protein kinase C gamma, indirectly regulates the dendritic development of Purkinje cells, cerebellar long term depression and ultimately cerebellar motor coordination (PubMed:32033984).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201196 | Dgkg (untagged) - Mouse diacylglycerol kinase, gamma (Dgkg), (10ug) |
CNY 2,000.00 |
|
| MR210661 | Dgkg (Myc-DDK-tagged) - Mouse diacylglycerol kinase, gamma (Dgkg) |
CNY 5,776.00 |
|
| MR210661L3 | Lenti ORF clone of Dgkg (Myc-DDK-tagged) - Mouse diacylglycerol kinase, gamma (Dgkg) |
CNY 7,700.00 |
|
| MR210661L4 | Lenti ORF clone of Dgkg (mGFP-tagged) - Mouse diacylglycerol kinase, gamma (Dgkg) |
CNY 7,700.00 |
