Usp1 (NM_146144) Mouse Tagged ORF Clone
CAT#: MG210640
- TrueORF®
Usp1 (tGFP-tagged) - Mouse ubiquitin specific peptdiase 1 (Usp1)
ORF Plasmid: DDK
"NM_146144" in other vectors (3)
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CNY 6,370.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210640 representing NM_146144
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGGCGTCATACCTAGTGAAAGTAATGGGCTTTCAAGAGGCAGTCCATCCAAGAAAAACAGACTGT CTCTGAAGTTTTTTCAGAAAAAGGAAACTAAAAGAGCCTTAGACTTTACTGATTCTCAAGAAAATGAAGA AAAAACTTCTGAATATAGAGGATCTGAGATTGACCAAGTTGTTCCTGCTGCACAGTCCTCCCCAGTAAGC TGCGAGAAGAGAGAGAACTTGTTACCATTTGTGGGATTGAATAACCTTGGCAACACTTGTTATCTGAATA GTATTCTTCAGGTGTTATACTTTTGTCCTGGCTTTAAGACTGGAGTGAAGCACTTATTTAATATTATTTC AAGGAAGAAAGAAGCTCTGAAGGATGACTCTAATCAGAAGGATAAGGGAAGCTGCAAAGAAGAGTCTTTG GCAAGTTATGAGCTTATATGTAGTTTACAGTCCTTAATAATTTCAGTTGAACAGCTTCAGGCTAGTTTTC TCTTAAATCCAGAGAAATATACAGATGAACTTGCTACACAGCCAAGGCGACTGCTGAACACACTCAGGGA ACTCAACCCTATGTATGAAGGATTTCTACAGCATGATGCACAGGAAGTGCTACAGTGTATTCTGGGAAAC ATTCAAGAAACATGCCAACTCCTAAAAAAAGAAGAAATAAAAAACCTGGCTGAATTATCCGGCAAGGTTG AAGAACAATCTCTTCAGAAAGAGGAAACAGGTGGAATTACCAGCACAGAGATTGACAGTATGAGGAATAC GGAGGACGTCAAAGAGCAGCTCCCCAAAGGGAACTGGAAAAGAAAAAGTGACAGTGAGTCGAGTAACGTG AAGAAAAAGGTTAAACTGTCCAGGGAATCCCAGCCATTGGAAGAAAACCAGAGACAAACCAGATCCAAGA GAAAAGCTATCGGAGACACACTAGAGGCTGCTCCTAAGATCATCCCCAAGTGTGTTTCTGAAAGTGAGAG TGCAAAGCCCTCACAGAAGAAATCAAAAGTTAAAATAAATTGGTTAAAGCCATCCACTAAGCAACCCAGC ATTCTTTCCAAGTTCTGTAGTCTTGGAAAAATAACAACTAACCAGCGCTCCAAAGGACAACCTAAGGAAA AGGAGGGTGATGTTGAAGAGGACTTGGAGAAGTACGGAAGTGACCACACAGCTAATGGTGGACCCGAATC TCCAGGAAGTAGCGTCACACCTGTGGACAGCAGTGAAGCCAAGTCCGGAAACAAAGGTGCTGAACAGATT GGCTTTGAGCTAGTAGAGAAACTGTTTCAAGGTCAGCTCGTGTTAAGGACTCGGTGTTTGGAATGTGAAA GCTTAACAGAAAGAAGAGAAGATTTCCAGGATATTAGTGTTCCAGTGCAAGAAGATGAGCTTTCCAAGGT CGAGGAGAGCTCTGAAATTTCTCCAGAGCCAAAAACAGAAATGAAGACCTTGAGATGGGCAATTTCACAG TTTGCTTCAGTGGAGAGAATTGTAGGAGAGGATAAATACTTCTGTGAAAATTGCCATCATTATACGGAAG CAGAGCGAAGTCTCCTGTTTGATAAAATGCCTGAAGTTATCACTATTCATCTGAAGTGCTTTGCTGCTAG TGGCTTGGAGTTTGACTGTTACGGTGGTGGACTTTCCAAGATCAACACTCCTTTATTGACACCTCTTAAA CTGTCACTAGAAGAATGGAGCACAAAGCCGACGAATGACAGCTATGGATTATTTGCCGTTGTGATGCACA GTGGCATTACTATTAGTAGTGGGCACTATACCGCATCTGTTAAAGTCACTGACCTTAACAGTCTAGAGCT CGATGAGGGGAATTTTGTGGTTGACCAGATGTGTGAACTAGGTAAGCCAGAGCCACTGACCGAGGAGCAA GCAAGGGGGACGGCTGGGAACTACGACGATGAAGTATCAATTAGAGTTGGTGGAAATGCCCAGCCAAGTA AAGTTTTGAACAAAAAAAATGTAGAAGGTATTGGACTTCTCGGTGGACAAAAGAGCAAAGCAGATTATGA GCTGTACAACAAAGCATCTAATCCTGATAAGGTGGTTGGGACACCATTCACCGACAATAGAAATTCTGAA ACTAATGATACTACTAATGGGACCCACGAATCTGACAGGAACAAGGAATCCAGTGACCAAACAGGCGTTA ACATGAACGGACTTGAGAACAAAATTTCCTATGTAGTGCAAAGCTTAAAGGAGTACGAGGGGAAGTGGTT GCTCTTTGACGATTCTGAGGTAAAAGTGACAGAAGAGAAAGACTTTCTGAATTCTCTCTCCCCTTCTACA TCCCCTACGTCTACTCCCTACTTGCTATTCTATAAAAAATTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210640 representing NM_146144
Red=Cloning site Green=Tags(s) MPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKTSEYRGSEIDQVVPAAQSSPVS CEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKTGVKHLFNIISRKKEALKDDSNQKDKGSCKEESL ASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGFLQHDAQEVLQCILGN IQETCQLLKKEEIKNLAELSGKVEEQSLQKEETGGITSTEIDSMRNTEDVKEQLPKGNWKRKSDSESSNV KKKVKLSRESQPLEENQRQTRSKRKAIGDTLEAAPKIIPKCVSESESAKPSQKKSKVKINWLKPSTKQPS ILSKFCSLGKITTNQRSKGQPKEKEGDVEEDLEKYGSDHTANGGPESPGSSVTPVDSSEAKSGNKGAEQI GFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQ FASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLK LSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDEGNFVVDQMCELGKPEPLTEEQ ARGTAGNYDDEVSIRVGGNAQPSKVLNKKNVEGIGLLGGQKSKADYELYNKASNPDKVVGTPFTDNRNSE TNDTTNGTHESDRNKESSDQTGVNMNGLENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPST SPTSTPYLLFYKKL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_146144 |
ORF Size | 2352 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_146144.4 |
RefSeq Size | 3567 bp |
RefSeq ORF | 2355 bp |
Locus ID | 230484 |
UniProt ID | Q8BJQ2 |
Gene Summary | This gene encodes a member of the ubiquitin-specific peptidase family. The encoded protein acts as a catalytic subunit in a heterodimeric deubiquitinating enzyme complex that deubiquitinates Fanconi anemia, complementation group D2, and plays a role in homologous recombination-mediated DNA repair. Disruption of this gene is associated with a Fanconi anemia-like phenotype and genomic instability. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 3, 12, and 15. [provided by RefSeq, Aug 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR210640 | Usp1 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 1 (Usp1) |
CNY 5,744.00 |
|
MR210640L3 | Lenti ORF clone of Usp1 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 1 (Usp1) |
CNY 7,700.00 |
|
MR210640L4 | Lenti ORF clone of Usp1 (mGFP-tagged) - Mouse ubiquitin specific peptidase 1 (Usp1) |
CNY 7,700.00 |