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Ccnf (NM_007634) Mouse Tagged ORF Clone
CAT＃: MG210593
- TrueORF®
Ccnf (tGFP-tagged) - Mouse cyclin F (Ccnf)
ORF Plasmid: DDK
"NM_007634" in other vectors (4)
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CNY 7,410.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CycF; Fbxo1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210593 representing NM_007634
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGCGGCGGTGTGATCCATTGTAGGTGTGCCAAGTGTTTCTGTTATCCTACTAAACGAAGAATCA AAAGAAGACCCAGAAACTTAACCATCTTGAGTCTCCCAGAAGATGTACTCTTTCATATCCTGAAATGGCT TTCTGTTGGGGACATCCTTGCTGTCCGAGCTGTCCACTCCCACCTCAAGTACCTGGTGGACAACCATGCC AGTGTGTGGGCATCCGCCAGCTTCCAGGAGCTGTGGCCTTCTCCACAGAACCTGAAGCTCTTTGAAAGGG CTGCTGAAAAGGGAAATTTTGAAGCTGCTGTGAAGTTGGGGATTGCCTACCTCTACAATGAAGGCCTGTC TGTGTCAGATGAGGCCTGCGCAGAAGTGAACGGCTTGAAGGCCTCTCGCTTCTTCAGCATGGCTGAGAGA CTGAATACGGGTTCTGAGCCCTTCATTTGGCTCTTCATCCGCCCACCGTGGTCAGTGTCCGGAAGCTGCT GCAAGGCTGTGGTTCATGACAGCCTCCGAGCGGAGTGTCAGTTACAGAGAAGTCATAAAGCTTCCATACT ACACTGCTTGGGAAGGGTGCTAAATCTTTTTGAGGACGAAGAGAAAAGGAAGCAGGCGCGTAGCCTTTTG GAAGAGTCTTCTCGTCAGGGATGCTTAATCAGCTCATACCTCCTTTGGGAAAGCGACAGAAAGATGTCCG ACCCCGGACGATGCCTCCACAGCTTCCGGAAACTCAGGGACTACGCTGCGAAAGGCTGCTGGGAAGCACA GCTGGCTTTGGCCAAAGCCTGTGCAGGTGGAAGCCAACTTGGACTGGAAGGGAAGGCCTGCAGTGAATCG GTCTGCCAGCTCTTCCAGGCCTCCCAGGCTGTCAACAAGCAGCAGATATTCTCTGTGCAGAAGGGGCTCA GCGACACCATGAGGTACATTCTAATCGACTGGTTGGTGGAAGTTGCCACAATGAAGGACTTTACAAGCCT GTGTCTTCACCTGACTGTGGAGTGTGTAGACCGGTACCTGCGAAGGAGGCTGGTCCCCAGATACAAGCTG CAGCTCCTGGGTATTGCCTGCATGGTCATCTGTACCCGGTTCATCAGCAAAGAGATTCTGACAATTAGAG AGGCTGTGTGGCTCACAGACAACACGTATAAATACGAGGACTTGGTGCGCGTGATGGGGGAGATCATCTC CGCCCTGGAAGGGAAGATTCGGATCCCTACCGTGGTTGACTATAAAGAGGTCCTGCTGACACTGGTCCCC GTCGCACCCAGAACCCAGCACCTGTGCAGCTTCCTCTGTGAGCTCACCCTGCTGCACACCAGCCTGTCCA TCTATGCCCCAGCCCGCCTGGCCTCTGCGGCCCTTCTCCTGGCCAGACTCATGCATGGGCAGACACAGCC CTGGACCACTCATCTGTGGGACCTCACTGGCTTCTCCTACAGTGACCTCGTACCCTGTGTCCTGAGCCTT CATAAGAAGTGTTTCCACGATGATGCACCCAAAGACTACAGACAAATCTCTCTGACGGCCGTGAAGCAGC GGTTCGAGGATAAGTGCTATGAGGAAATCAGCCGGGAAGAGGTGCTGAGCTATGCTGACTTGTGCAGTGC AATAGGAGTAAAACAGGAAAGCCCAGAGCCCCCATCTTTCCCTAGCTCAGGGGAGATCCACACCTTCCTC AGCTCGCCCTCCGGGAGGAGGAGCAAACGGAAGCGAGAGAACAGCCTTCAGGAGGACAGAGGCAGCTTTG TCACCACACCCACCGCAGAGCTATCGAATCAGGAGGAGACATTGCTAGGCAGCCTTCTGGACTGGAGCCT GGAGTGCTGCTCTGGCTATGAGGGAGACCAGGAGAGCGAAGGCGAGAAGGAGGGTGATGTCACAGCTCCC AGCAGACTCCTTGATGTCACTGTGGTCTACCTGAACCCAGAAGAACATTGCTGCCAGGAGTCCAGTGATG AGGAGGCCTGGCCAGAGGACAAGATCCACCCAGCACCAGGCACCCAGGCACCTCCAGCCTCAGCTCCCAG GCCCCTTCTCTGCAATCGGGGAGATCGGGCCAAGGACATTACGACCTCAGGATACTCCTCTGTTAGCAGC TCGAGTCCCATAAGCTCCCTGGATGGTGGCATGGGGGGCTCTCCCCAATCTACCTCAGTGCTCTCTGTGG GCAGCCACTCAAGCACAAAGCCTTGCCACCATCAGGCCAAGAAGTCATGTTTACAGTGTCGTCCCCCAAA TTCCCCAGAGAGCGGTGTTCACCAGCAGCCTGTAAAGCGCCAAAACCTGTCTGTACACAGTGACAAGGAC ACGCACCTGGCTTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210593 representing NM_007634
Red=Cloning site Green=Tags(s) MGSGGVIHCRCAKCFCYPTKRRIKRRPRNLTILSLPEDVLFHILKWLSVGDILAVRAVHSHLKYLVDNHA SVWASASFQELWPSPQNLKLFERAAEKGNFEAAVKLGIAYLYNEGLSVSDEACAEVNGLKASRFFSMAER LNTGSEPFIWLFIRPPWSVSGSCCKAVVHDSLRAECQLQRSHKASILHCLGRVLNLFEDEEKRKQARSLL EESSRQGCLISSYLLWESDRKMSDPGRCLHSFRKLRDYAAKGCWEAQLALAKACAGGSQLGLEGKACSES VCQLFQASQAVNKQQIFSVQKGLSDTMRYILIDWLVEVATMKDFTSLCLHLTVECVDRYLRRRLVPRYKL QLLGIACMVICTRFISKEILTIREAVWLTDNTYKYEDLVRVMGEIISALEGKIRIPTVVDYKEVLLTLVP VAPRTQHLCSFLCELTLLHTSLSIYAPARLASAALLLARLMHGQTQPWTTHLWDLTGFSYSDLVPCVLSL HKKCFHDDAPKDYRQISLTAVKQRFEDKCYEEISREEVLSYADLCSAIGVKQESPEPPSFPSSGEIHTFL SSPSGRRSKRKRENSLQEDRGSFVTTPTAELSNQEETLLGSLLDWSLECCSGYEGDQESEGEKEGDVTAP SRLLDVTVVYLNPEEHCCQESSDEEAWPEDKIHPAPGTQAPPASAPRPLLCNRGDRAKDITTSGYSSVSS SSPISSLDGGMGGSPQSTSVLSVGSHSSTKPCHHQAKKSCLQCRPPNSPESGVHQQPVKRQNLSVHSDKD THLAS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007634 |
| ORF Size | 2331 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007634.2, NP_031660.2 |
| RefSeq Size | 3721 bp |
| RefSeq ORF | 2334 bp |
| Locus ID | 12449 |
| UniProt ID | P51944 |
| Gene Summary | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203265 | Ccnf (untagged) - Mouse cyclin F (Ccnf), (10ug) |
CNY 5,688.00 |
|
| MR210593 | Ccnf (Myc-DDK-tagged) - Mouse cyclin F (Ccnf) |
CNY 5,688.00 |
|
| MR210593L3 | Lenti ORF clone of Ccnf (Myc-DDK-tagged) - Mouse cyclin F (Ccnf) |
CNY 8,650.00 |
|
| MR210593L4 | Lenti ORF clone of Ccnf (mGFP-tagged) - Mouse cyclin F (Ccnf) |
CNY 8,088.00 |
