Lima1 (BC031490) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Eplin
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210477 representing BC031490 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATCGACTCCATTTAATAGACGCCAGTGGACTTCCCTGTCATTGAGAGTAACAGCCAAAGAGCTTT CTCTTGTCAACAAGAACAAGTCATCCGCAATTGTGGAAATATTCTCCAAGTACCAGAAGGCTGCTGAAGA AGCCAACATGGAAAGGAAGAAAAATAACCCTGAAAGTCTGCCCCAGCACTTTAGAAGAGGGACCCTGTCT GTGTTAAAGAAGAAGTGGGAGAACCCGGTGGCTGGGGCAGAATTCCACACAGACTCACTGCCAAACAGCA GCAGTGAGGGTGGGCACACAGCGGACTACCCTCCTGCTGAAGTGACGGACAAGCCTGCTCCTGGAGTCAG AGCTGACCGGGAAGAGCACACCCAGCCCAAACCTAGATTTGGATCTCGTCCGGAAGCAGTTATCCAGAGC CGGTATCCTCGCTCAGAGAACAGCCACGATTTTAAAGCCCAGGCCACGGAGAGCCAAAAAATGGAAAACT GTCTGGGAGATTCCAGGCATGAAGCAGAGAAGCCAGAGACGAGCGAAAACACAGAAACTTCAGGCAAAAT AGAGAAATACAACGTTCCACTGAATAGACTGAAGATGATGTTTGAGAAAGGTGAACACAACCAAACCAAG AGTCTCTGGACCCAAAGCCGAAATGCGGGTGGAAGGAGGCTCTCTGAAAACAACTGTTCCCTGGATGACT GGGAAATAGGTGCGGGTCATTTGTCTTCCTCTGCATTCAACTCGGAGAAAAACGAGAGTAAGAGGAATCT GGAGCTGCCACGCCTCTCAGAAACCTCCATAAAGGACCGCATGGCCAAGTACCAGGCTGCAGTGTCCAAG CAGAGCAGCCCAGCCAGCTACACAAATGAGCTGAAAACCAGTGAAAGCAAAACTCATAAATGGGAACAGA AGGAGAATGTGCCCCCAGGTCCCGAGGCCTGCAGCGTCCATCAGGAAGGAAGCAAGGTTTCTACAACTGA GAATAGTCTAGTGGCCCTCTCTGTCCCTGCTGAAGATGACACCTGTAACTCCCAGGTGAAGAGCGAGGCC CAGCAGCCTATGCACCCTAAGCCGCTGAGTCCTGACGCCAGGACCTCCAGTCTTCCGGAAAGTTCTCCTT CCAAAACAGCGAAGAAGTTTCAGGCGCCGGCAAAGGAGAGCTGCGTGGAGTGTCAGAAGACGGTGTACCC CATGGAACGGCTCCTGGCCAACCAGCAGGTGTTTCACATCAGCTGTTTCCGATGCTCCTACTGCAACAAC AAGCTCAGTCTAGGAACATATGCATCCTTACATGGACGAATCTACTGTAAGCCTCACTTCAATCAACTCT TCAAGTCCAAAGGCAACTATGACGAGGGCTTTGGGCACAAGCAGCATAAGGATCTGTGGGCAAGCAAGAG CGACAATGAGGAGACTTTGGGGAGACCAGCCCAGCCGCCTAATGCAGGGGAGAGCCCCCATAGCCCGGGT GTAGAAGATGCCCCCATCGCCAAGGTCGGCGTGCTGGCGGCAAGTATGGAAGCCAAGGCCTCTTCTCAGA GGGAGAGGGAGGATAAGCCTGCGGAGACCAAGAAGCTGAGGATCGCCTGGCCTCCGCCAGCCGAGCTGGG CGGTTCCGGAAGTGCCCTGGAGGAAGGGATCAAAGTATCGAAGCCCAAGTGGCCTCCGGAGGATGACGTC TGCAAGACGGAGGCCCCGGAGGATGTAGATCTGGACCTGAAGAAGCTGCGGCGGTCTTCGTCCCTGAAGG AGAGGAGCCGCCCGTTTACGGTAGCAGCTTCCTTTCGAACCTCTTCCATCAAGAGCCCCAAGGCCTCTTC GCCATCTCTCAGGAAAGGTTGGAGCGAGTCCGAGCAGAGTGAGGAGTTTGGAGGAGGGATAGCGACGATG GAAAGGAAACAAACGGAAAATGCCAGACCCTCTGGGGAGAAAGAGAATGTGGGAAAATCACGCTGGCAGG GCGAGGAAGTGCCGAGGAGCAAGGACCGTAGTTCTTTTGAGCTGGAGAGTGAGAATTTTATGGAAAATGG AGCAAACATAGCTGAAGATGACAACCATGTCCACGCACAGCAGTCCCCGTTAGAACCCGAGGCTCCGGGT TGGTCTGGCTTTGTAGACACCACGGCTGCTAAAGAATTCACTACCCAGAATCAAAAATCCCAAGATGTCG GGTTCTGGGAGGGAGAAGTGGTCCGAGAGCTGTCTGTGGAGGAACAGATAAAGAGAAACCGGTACTATGA TGAGGACGAGGATGAAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210477 representing BC031490 Red=Cloning site Green=Tags(s) MESTPFNRRQWTSLSLRVTAKELSLVNKNKSSAIVEIFSKYQKAAEEANMERKKNNPESLPQHFRRGTLS VLKKKWENPVAGAEFHTDSLPNSSSEGGHTADYPPAEVTDKPAPGVRADREEHTQPKPRFGSRPEAVIQS RYPRSENSHDFKAQATESQKMENCLGDSRHEAEKPETSENTETSGKIEKYNVPLNRLKMMFEKGEHNQTK SLWTQSRNAGGRRLSENNCSLDDWEIGAGHLSSSAFNSEKNESKRNLELPRLSETSIKDRMAKYQAAVSK QSSPASYTNELKTSESKTHKWEQKENVPPGPEACSVHQEGSKVSTTENSLVALSVPAEDDTCNSQVKSEA QQPMHPKPLSPDARTSSLPESSPSKTAKKFQAPAKESCVECQKTVYPMERLLANQQVFHISCFRCSYCNN KLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHKQHKDLWASKSDNEETLGRPAQPPNAGESPHSPG VEDAPIAKVGVLAASMEAKASSQREREDKPAETKKLRIAWPPPAELGGSGSALEEGIKVSKPKWPPEDDV CKTEAPEDVDLDLKKLRRSSSLKERSRPFTVAASFRTSSIKSPKASSPSLRKGWSESEQSEEFGGGIATM ERKQTENARPSGEKENVGKSRWQGEEVPRSKDRSSFELESENFMENGANIAEDDNHVHAQQSPLEPEAPG WSGFVDTTAAKEFTTQNQKSQDVGFWEGEVVRELSVEEQIKRNRYYDEDEDEE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC031490
ORF Size	2261 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC031490, AAH31490
RefSeq Size	3232 bp
RefSeq ORF	2261 bp
Locus ID	65970
Gene Summary	Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (By similarity). Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (PubMed:29880681).[UniProtKB/Swiss-Prot Function]

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