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Med15 (NM_001040683) Mouse Tagged ORF Clone
CAT＃: MG210454
- TrueORF®
Med15 (tGFP-tagged) - Mouse positive cofactor 2, multiprotein complex, glutamine/Q-rich-associated protein (Pcqap), transcript variant 2
ORF Plasmid: DDK
"NM_001040683" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A230074L19Rik; AW536074; mPcqap; Pcqap |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210454 representing NM_001040683
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTTTCGGGGCAGGAGACCGACTGGCGTAGCGCCGCCTTTCGGCAGAAGCTGGTCAGCCAAATTG AGGATGCCATGAGGAAAGCTGGTGTGGCCCACAGTAAATCTAGCAAGGATATGGAGAGTCATGTGTTCCT GAAGGCCAAGACCCGGGATGAGTATCTTTCCCTTGTGGCCCGACTCATTATCCATTTCCGAGATATTCAT AACAAGAAATCCCAAGCTTCTGTCAGTGACCCCATGAATGCACTGCAGAGCCTTACTGGTGGACCCACCC CAGGAGCAGCTGGGATTGGCATGCCTCCTCGGGGCCCAGGACAGTCCCTGGGTGGGATGGGTGGCCTTGG CGCTATGGGACAACCACTGCCCCTCTCCGGGCAACCACCCCCTGGAACCTCTGGAATGGCCCCTCATGGC ATGGCTGTGGTGTCTACAGCAACTCCACAGACTCAGCTGCAGCTCCAGCAAGTGGCATTGCAGCAACAGC AGCAGCAGCAACAACAACAGCAATTCCAGCAACAGCAGGCAGCACTGCAGCAACATCAGCAACAGCAGCA GCAGCAACAGCAGCAACAGCAGTTCCAGGCACAACAGAATGCCATGCAGCAACAGTTCCAAGCAGTAGTG CAGCAGCAGCAGCTTCAGCAGCAGCAGCAGCAGCAGCACCTGATTAAGTTGCATCATCAAAGCCAGCAAC AACAGATACAACAGCAGCAACTGCAGAGGATGGCACAGTTGCAGCTGCAGCAACAGCAACAGCAGCAACA GCAGCAGGCTTTGCAGGCCCAGCCACCAATGCAGCAGCCATCAATGCAGCAGCCACAGCCTCCCCCTTCT CAGGCCCTACCCCAGCAGCTGTCACAGCTGCATCATCCACAGCATCACCAGCCACCACCTCAGGCTCAGC AGTCCCCCATTGCTCAAAACCAACCACCACAGATCCCATCACAGTCACAGAGCCAGCCTTTGGTGTCACA AGCACAAGCCCTTCCTGGACCGATGCTGTATGCTGCCCAACAGCAGCTGAAATTTGTCCGTGCTCCGATG GTGGTCCAGCAGCCGCAAGTGCAGCCCCAGGTGCAGCAGGTGCAGCCCCAGGTGCAGCCGCAGGCAGCAG TGCAGGCAGCACAGTCTGCCCAGATGGTAGCTCCCGGCGTCCAGGTCAGCCAAAGCAGCCTCACCATGCT GTCCTCACCGTCACCAGGCCAGCAGGTGCAGACCCCACAGTCGATGCCACCTCCCCCACAGCCGTCCCCA CAACCTGGCTCACAGCCCAACTCTAATGTCAGCTCCGGCCCTGCCCCATCTCCCAGCAGCTTCCTGCCTA GCCCTTCACCACAGCCTTCTCAGAGCCCAGTGACAGCACGCACCCCACAGAACTTCAGCGTTCCTTCCCC TGGACCTTTAAACACCCCTGTGAACCCCAGCTCTGTCATGAGCCCAGCTGGCTCTAGCCAGGCTGAGGAG CAGCAGTACCTGGACAAGCTGAAGCAATTGTCCAAGTACATCGAGCCCCTGCGACGCATGATCAACAAGA TCGACAAGAATGAAGACAGAAAAAAGGACTTAAGTAAGATGAAGAGCCTGCTGGACATCCTCACCGACCC CTCGAAGAGGTGTCCCCTGAAGACCCTGCAAAAGTGTGAGATTGCGCTGGAGAAGCTCAAGAATGACATG GCAGTGCCCACACCCCCACCACCCCCAGTTCTTCCAACCAAACAGCAGGACCTGTGCCAACCACTCCTAG ATGCAGTCCTGGCCAACATCCGTTCACCTGTCTTCAACCATTCCCTGTACCGCACATTTGTGCCAGCCAT GATGGCCATCCATGGCCCACCTATCGTGTCGCCAGTGGTGTGTTCCCGGAAGCGCCGGTTTGAGGAGGAT GAGCGGCAGAGCATACCCAATGTGCTGCAAGGTGAAGTGGCAAGGCTGGATCCCAAGTTCCTGGTGAACT TGGACCCTTCTCACTGCAGCAACAACGGTACTGTCCACCTGATCTGCAAGCTGGATGATAAGGACCTCCC TAGTGTGCCACCACTGGAGCTCAGTGTGCCTGCTGACTACCCTGCCCAGAGCCCAATGTGGATCGACCGT CAGTGGCAATATGATGCCAACCCCTTTCTGCAGTCAGTGCACCGGTGCATGACCTCAAGGCTGCTGCAGC TCCCTGACAAGCACTCGGTCACAGCGCTGCTCAACACCTGGGCCCAGAGCATCCACCAGGCCTGCCTCTC AGCTGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210454 representing NM_001040683
Red=Cloning site Green=Tags(s) MDVSGQETDWRSAAFRQKLVSQIEDAMRKAGVAHSKSSKDMESHVFLKAKTRDEYLSLVARLIIHFRDIH NKKSQASVSDPMNALQSLTGGPTPGAAGIGMPPRGPGQSLGGMGGLGAMGQPLPLSGQPPPGTSGMAPHG MAVVSTATPQTQLQLQQVALQQQQQQQQQQQFQQQQAALQQHQQQQQQQQQQQQFQAQQNAMQQQFQAVV QQQQLQQQQQQQHLIKLHHQSQQQQIQQQQLQRMAQLQLQQQQQQQQQQALQAQPPMQQPSMQQPQPPPS QALPQQLSQLHHPQHHQPPPQAQQSPIAQNQPPQIPSQSQSQPLVSQAQALPGPMLYAAQQQLKFVRAPM VVQQPQVQPQVQQVQPQVQPQAAVQAAQSAQMVAPGVQVSQSSLTMLSSPSPGQQVQTPQSMPPPPQPSP QPGSQPNSNVSSGPAPSPSSFLPSPSPQPSQSPVTARTPQNFSVPSPGPLNTPVNPSSVMSPAGSSQAEE QQYLDKLKQLSKYIEPLRRMINKIDKNEDRKKDLSKMKSLLDILTDPSKRCPLKTLQKCEIALEKLKNDM AVPTPPPPPVLPTKQQDLCQPLLDAVLANIRSPVFNHSLYRTFVPAMMAIHGPPIVSPVVCSRKRRFEED ERQSIPNVLQGEVARLDPKFLVNLDPSHCSNNGTVHLICKLDDKDLPSVPPLELSVPADYPAQSPMWIDR QWQYDANPFLQSVHRCMTSRLLQLPDKHSVTALLNTWAQSIHQACLSAA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001040683 |
| ORF Size | 2247 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001040683.1, NP_001035773.1 |
| RefSeq Size | 3263 bp |
| RefSeq ORF | 2250 bp |
| Locus ID | 94112 |
| Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202625 | Med15 (untagged) - Mouse mediator complex subunit 15 (Med15), transcript variant 2, (10ug) |
CNY 5,496.00 |
|
| MR210454 | Med15 (Myc-DDK-tagged) - Mouse mediator complex subunit 15 (Med15), transcript variant 2 |
CNY 5,496.00 |
|
| MR210454L3 | Lenti ORF clone of Med15 (Myc-DDK-tagged) - Mouse mediator complex subunit 15 (Med15), transcript variant 2 |
CNY 7,700.00 |
|
| MR210454L4 | Lenti ORF clone of Med15 (mGFP-tagged) - Mouse mediator complex subunit 15 (Med15), transcript variant 2 |
CNY 7,700.00 |
