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Polk (BC052820) Mouse Tagged ORF Clone
CAT＃: MG210382
- TrueORF®
Polk (tGFP-tagged) - Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338)
ORF Plasmid: DDK
"BC052820" in other vectors (4)
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CNY 4,560.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Dinb1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210382 representing BC052820
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAACACAAAGGAAAAGGACAACTTCAAAGACGACCTCCTGCTCCGCATGGGACTAAACGATAACA AAGCAGGCATGGAAGGGTTGGATAAGGAGAAAATTAACAAAATTATCATGGAAGCCACAAAGGGGTCCAG ATTTTATGAATATGATCCCAATTTTATGGCCATGAGTCTGGACGAAGCCTACTTGAATATAACACAGCAC TTGCAGGAAAGGCAAGATTGGCCTGAGGACAAAAGAAGATACTTCATCAAAATGGGAAACTACTTAAAAA TCGACACACCCAGACAGGAAGCTAACGAGCTGACTGAGTATGAGCGGTCCATCTCCCCGCTGCTTTTTGA AGATAGTCCTCCTGATTTGCAACCCCAAGGAAGTCCTTTCCAACTGAACTCTGAAGAACAAAACAATCCT CAAATAGCCCAAAATTCAGTTGTTTTTGGAACATCAGCTGAGGAAGTGGTAAAGGAAATTCGCTTCAGAA TTGAACAAAAAACAACGCTGACAGCCAGCGCAGGCATCGCCCCCAATACAATGTTAGCAAAAGTGTGCAG TGATAAGAATAAGCCAAACGGACAGTACCAGATCCTTCCCAGCAGGAGCGCGGTGATGGACTTCATCAAG GACCTGCCTATTAGAAAGGTTTCTGGGATAGGAAAAGTTACAGAGAAAATGTTAATGGCTCTCGGGATTG TTACTTGCACAGAACTCTACCAACAGAGAGCGTTGCTGTCTCTCCTTTTCTCTGAAACCTCTTGGCATTA TTTTCTTCACATCGCGCTGGGTCTAGGTTCAACAGACCTGGCAAGGGATGGAGAAAGGAAAAGCATGAGT GTTGAAAGGACATTCAGTGAGATAAGTAAGACAGAGGAACAGTACAGCCTGTGCCAAGAACTGTGCGCTG AGCTCGCCCACGACCTCCAGAAGGAAGGACTTAAGGGAAGAACCGTCACCATTAAGCTGAAGAACGTGAA TTTTGAAGTAAAAACTCGTGCATCTACCGTTCCGGCCGCCATTTCTACTGCAGAGGAAATATTTGCCATT GCCAAGGAGCTGCTAAGGACAGAAGTTAATGTGGGTTCTCCACACCCCCTGCGGTTAAGACTGATGGGTG TCCGAATGTCTACTTTTTCCAGTGAAGATGACAGGAAACACCAACAAAGGAGCATCATTGGTTTCTTACA AGCTGGAAACCAAGCTTTGTCATCTACTGGGGGTAGTCTAGACAAAACTGCCAAAACTGAGCTTGCAAAG CCCTTAGAAATGTCTCATAAGAAGAGTTTCTTTGATAAAAAGCGATCAGAAAGAATCTCCAACTGTCAAG ACACATCCAGATGTAAAACTGCGGGTCAGCAAGCTTTACAGATCTTGGAACCATCCCAAGCATTAAAGAA GCTGAGCCAGAGTTTTGAAACATCAGAGAATTCAAATGACTGTCAGACATTTATATGTCCAGTTTGCTTT AGGGAGCAAGAAGGTGTCAGTCTGGAAGCCTTTAATGAACATGTAGATGAGTGTCTTGATGGACCGTCAA CCAGTGAGAACTCAAAAATATCCTGTTACTCACATGCTTCCTCTGCAGACATTGGTCAGAAGGAAGATGT ACACCCCTCTATTCCACTGTGTGAGAAACGGGGGCATGAAAATGGAGAGATCACTTCAGTAGATGGTGTA GATCTAACAGGGACGGAAGACAGATCATTGAAAGCAGCAAGTATGGACACTCTAGAGAATAATCGCAGCA AAGAGGAATGTCCTGATATTCCAGACAAGTCTTGTCCTATATCACTGGCAAATGAAACCATCAGTACATT AAGTAGGCAAGAATCTGTCCAGCCTTGTACAGATGAGGTAGTAACAGGACGAGCTCTAGTGTGTCCTGTT TGTAACCTAGAACAAGAGACTTCTGATCTTACCCTCTTCAACATACATGTGGATATTTGCTTAAATAAAG GTATTATCCAAGAACTGAGAAATAGTGAAGGTAATTCAGTTAAACAACCCAAAGAAAGCTCGAGAAGTAC TGACAGACTTCAGAAGGCTTCAGGAAGGACCAAAAGGCCACGTGGAGCAACCTCACCATCAGCTCCTCCA TGCTTAGCCACCATGGTACCATTGATGCTTCCGTACCTACCTGTGTGCATCTCTGTGTCCTGTGAGCAGA TGCAGTTAGAATTGGCCATGATGTCCATGAAGAACATTAGCTTAACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210382 representing BC052820
Red=Cloning site Green=Tags(s) MDNTKEKDNFKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYEYDPNFMAMSLDEAYLNITQH LQERQDWPEDKRRYFIKMGNYLKIDTPRQEANELTEYERSISPLLFEDSPPDLQPQGSPFQLNSEEQNNP QIAQNSVVFGTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSRSAVMDFIK DLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMS VERTFSEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAI AKELLRTEVNVGSPHPLRLRLMGVRMSTFSSEDDRKHQQRSIIGFLQAGNQALSSTGGSLDKTAKTELAK PLEMSHKKSFFDKKRSERISNCQDTSRCKTAGQQALQILEPSQALKKLSQSFETSENSNDCQTFICPVCF REQEGVSLEAFNEHVDECLDGPSTSENSKISCYSHASSADIGQKEDVHPSIPLCEKRGHENGEITSVDGV DLTGTEDRSLKAASMDTLENNRSKEECPDIPDKSCPISLANETISTLSRQESVQPCTDEVVTGRALVCPV CNLEQETSDLTLFNIHVDICLNKGIIQELRNSEGNSVKQPKESSRSTDRLQKASGRTKRPRGATSPSAPP CLATMVPLMLPYLPVCISVSCEQMQLELAMMSMKNISLT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC052820 |
| ORF Size | 2219 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052820, AAH52820 |
| RefSeq Size | 3517 bp |
| RefSeq ORF | 2219 bp |
| Locus ID | 27015 |
| Gene Summary | DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls (PubMed:12432099). Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220775 | Polk (untagged) - Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338), (10ug) |
CNY 9,120.00 |
|
| MR210382 | Polk (Myc-DDK-tagged) - Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338) |
CNY 5,416.00 |
|
| MR210382L3 | Lenti ORF clone of Polk (Myc-DDK-tagged) - Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338) |
CNY 6,080.00 |
|
| MR210382L4 | Lenti ORF clone of Polk (mGFP-tagged) - Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338) |
CNY 6,080.00 |
