Arhgap17 (BC003259) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Wbp15, Nadrin, Rich1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210303 representing BC003259 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAGCAATTCAACCGCATGAAGCAGCTGGCCAATCAGACTGTCGGCAGAGCTGAGAAGACCGAAG TCCTCAGTGAAGATCTGCTACAGATTGAACGTCGCCTGGACACTGTGCGTTCAATGTGCCACCATTCACA TAAGCGTTTGATAGCCTGCTTCCAAGGTCAGCATGGCACTGATGCCGAGAGGAGACATAAAAAGCTTCCT CTGACAGCTCTTGCCCAGAACATGCAGGAGGCTTCAGCCCAGCTGGAAGAGTCTCTCTTGGGGAAGATGC TGGAGACCTGTGGGGACGCTGAGAACCAGCTGGCTCTTGAACTCTCACAACATGAAGTCTTTGTGGAGAA GGAGATCATGGACCCTCTCTACGGCATAGCAGAGGTGGAGATTCCCAATATCCAGAAGCAAAGGAAGCAG CTTGCTAGGTTGGTGTTGGACTGGGATTCAGTCAGAGCGAGATGGAACCAAGCACACAAGTCTTCAGGAA CCAACTTTCAGGGGCTTCCATCCAAAATAGATACCCTAAAGGAAGAGATGGATGAAGCTGGGAATAAAGT TGAACAGTGCAAGGATCAACTTGCAGCAGACATGTACAACTTCATGGCCAAAGAAGGGGAGTATGGCAAG TTCTTCGTGACGTTATTAGAAGCCCAAGCAGATTACCATAGAAAAGCATTAGCAGTCTTAGAAAAGGCCC TTCCCGAAATGCGCGCCCATCAAGATAAGTGGGCAGAGAAGCCAGCCTTCGGCACACCTCTGGAGGAACA CCTGAAGAGGAGTGGGCGTGAGATCGCCCTCCCTATTGAGGCCTGTGTCATGTTGTTGCTGGAGACTGGC ATGAAAGAGGAGGGCCTTTTTCGGATTGGAGCTGGAGCCTCCAAGTTGAAGAAGCTGAAAGCTGCTCTCG ACTGCTCCACATCGCATCTGGATGAATTCTATTCTGATCCCCATGCTGTCGCAGGTGCTTTAAAGTCCTA TCTGCGGGAGTTGCCTGAGCCCTTAATGACTTTCAGTCTTTATGAAGAATGGACACAAGTTGCAAGTGTG CAGGATCAAGACAAAAAACTTCAATATTTATGGACAACATGTCAGAAGTTGCCGCCACAAAATTTTGTTA ACTTTAGGTATTTAATCAAGTTCCTTGCAAAGCTTGCCCAGACCAGTGACGTTAATAAAATGACTCCTAG CAACATAGCCATTGTCCTGGGCCCTAACCTCCTGTGGGCCAAACAAGAAGGAACGCTGGCTGAAATAGCA GCTGCCACATCGGTCCACGTGGTTGCGGTGATTGAGCCCATCATCCAGCATGCAGATTGGTTCTTCCCTG GAGAGGTAGAATTCAATGTATCAGAAGCATTTGTGCCACTTGCTACCCCGAATTCTAATCACTCATCCCA TACTGGAAATGACTCTGACTCGGGGACTCTGGAGAGGAAGCGACCTGCCAGCATGGCAGTGATGGAAGGG GACTTGGTGAAGAAGGAGAGTCCTCCAAAACCCAAAGACTCCGTGTCTGCAGCTGTCCCTGCAGCAGGAA GGAACAGCAACCAGATGACCACGGTCCCAAACCAGGCTCAGACAGGTGGCAACTCCCATCAGCTCTCAGT AAGCACACCTCACAGTGCAGCCGGTCCCAGCCCGCACACTCTGCGTCGGGCTGTGAAGAAACCTGCCCCC GCACCCCCCAAACCAGGAAACCTACCTCCTGGCCACCCTGGAGGACAGAGCTCTCCTGGCACAGGCACAT CCCCAAAGCCAAGCGCCCGAAGCCCATCACCGCCTCAGCAGCAGCAACAACAGCAGCAACAACAGCAGCA GCAGCAGACCCCAGGCATGCGCCGCTGCTCCAGCAGCCTGCCTCCCATCCAGGCACCCAGCCACCCACCA CCACAGCCCCCCACACAGCCTCGGTTGGGTGAACAGGGACCAGAGCCAGGCCCCACGCCACCTCAAACCC CCACACCACCTAGCACCCCACCCCTGGCCAAGCAGAATCCATCGCAAAGTGAGACCACACAGCTGCACGG AACCCTCCCAAGACCACGGCCAGTGCCCAAGCCCCGCAACCGGCCTAGCGTGCCACCACCCCCACATCCA CCTGGCACCCACACGGTGGATGGTGGCCTTACATCCTCAGTGCCCACAGCCTCCAGAATCGTCACTGATG TA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210303 representing BC003259 Red=Cloning site Green=Tags(s) MKKQFNRMKQLANQTVGRAEKTEVLSEDLLQIERRLDTVRSMCHHSHKRLIACFQGQHGTDAERRHKKLP LTALAQNMQEASAQLEESLLGKMLETCGDAENQLALELSQHEVFVEKEIMDPLYGIAEVEIPNIQKQRKQ LARLVLDWDSVRARWNQAHKSSGTNFQGLPSKIDTLKEEMDEAGNKVEQCKDQLAADMYNFMAKEGEYGK FFVTLLEAQADYHRKALAVLEKALPEMRAHQDKWAEKPAFGTPLEEHLKRSGREIALPIEACVMLLLETG MKEEGLFRIGAGASKLKKLKAALDCSTSHLDEFYSDPHAVAGALKSYLRELPEPLMTFSLYEEWTQVASV QDQDKKLQYLWTTCQKLPPQNFVNFRYLIKFLAKLAQTSDVNKMTPSNIAIVLGPNLLWAKQEGTLAEIA AATSVHVVAVIEPIIQHADWFFPGEVEFNVSEAFVPLATPNSNHSSHTGNDSDSGTLERKRPASMAVMEG DLVKKESPPKPKDSVSAAVPAAGRNSNQMTTVPNQAQTGGNSHQLSVSTPHSAAGPSPHTLRRAVKKPAP APPKPGNLPPGHPGGQSSPGTGTSPKPSARSPSPPQQQQQQQQQQQQQQTPGMRRCSSSLPPIQAPSHPP PQPPTQPRLGEQGPEPGPTPPQTPTPPSTPPLAKQNPSQSETTQLHGTLPRPRPVPKPRNRPSVPPPPHP PGTHTVDGGLTSSVPTASRIVTDV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC003259
ORF Size	2174 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003259, AAH03259
RefSeq Size	3146 bp
RefSeq ORF	2174 bp
Locus ID	70497
Gene Summary	Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1 (By similarity).[UniProtKB/Swiss-Prot Function]

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