Arhgap17 (BC003259) Mouse Tagged ORF Clone

CAT#: MG210303

  • TrueORF®

Arhgap17 (tGFP-tagged) - Mouse Rho GTPase activating protein 17 (cDNA clone MGC:6676 IMAGE:3500822)



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Mouse Protein / Lysate Request

CNY 6,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Wbp15, Nadrin, Rich1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210303 representing BC003259
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAAGCAATTCAACCGCATGAAGCAGCTGGCCAATCAGACTGTCGGCAGAGCTGAGAAGACCGAAG
TCCTCAGTGAAGATCTGCTACAGATTGAACGTCGCCTGGACACTGTGCGTTCAATGTGCCACCATTCACA
TAAGCGTTTGATAGCCTGCTTCCAAGGTCAGCATGGCACTGATGCCGAGAGGAGACATAAAAAGCTTCCT
CTGACAGCTCTTGCCCAGAACATGCAGGAGGCTTCAGCCCAGCTGGAAGAGTCTCTCTTGGGGAAGATGC
TGGAGACCTGTGGGGACGCTGAGAACCAGCTGGCTCTTGAACTCTCACAACATGAAGTCTTTGTGGAGAA
GGAGATCATGGACCCTCTCTACGGCATAGCAGAGGTGGAGATTCCCAATATCCAGAAGCAAAGGAAGCAG
CTTGCTAGGTTGGTGTTGGACTGGGATTCAGTCAGAGCGAGATGGAACCAAGCACACAAGTCTTCAGGAA
CCAACTTTCAGGGGCTTCCATCCAAAATAGATACCCTAAAGGAAGAGATGGATGAAGCTGGGAATAAAGT
TGAACAGTGCAAGGATCAACTTGCAGCAGACATGTACAACTTCATGGCCAAAGAAGGGGAGTATGGCAAG
TTCTTCGTGACGTTATTAGAAGCCCAAGCAGATTACCATAGAAAAGCATTAGCAGTCTTAGAAAAGGCCC
TTCCCGAAATGCGCGCCCATCAAGATAAGTGGGCAGAGAAGCCAGCCTTCGGCACACCTCTGGAGGAACA
CCTGAAGAGGAGTGGGCGTGAGATCGCCCTCCCTATTGAGGCCTGTGTCATGTTGTTGCTGGAGACTGGC
ATGAAAGAGGAGGGCCTTTTTCGGATTGGAGCTGGAGCCTCCAAGTTGAAGAAGCTGAAAGCTGCTCTCG
ACTGCTCCACATCGCATCTGGATGAATTCTATTCTGATCCCCATGCTGTCGCAGGTGCTTTAAAGTCCTA
TCTGCGGGAGTTGCCTGAGCCCTTAATGACTTTCAGTCTTTATGAAGAATGGACACAAGTTGCAAGTGTG
CAGGATCAAGACAAAAAACTTCAATATTTATGGACAACATGTCAGAAGTTGCCGCCACAAAATTTTGTTA
ACTTTAGGTATTTAATCAAGTTCCTTGCAAAGCTTGCCCAGACCAGTGACGTTAATAAAATGACTCCTAG
CAACATAGCCATTGTCCTGGGCCCTAACCTCCTGTGGGCCAAACAAGAAGGAACGCTGGCTGAAATAGCA
GCTGCCACATCGGTCCACGTGGTTGCGGTGATTGAGCCCATCATCCAGCATGCAGATTGGTTCTTCCCTG
GAGAGGTAGAATTCAATGTATCAGAAGCATTTGTGCCACTTGCTACCCCGAATTCTAATCACTCATCCCA
TACTGGAAATGACTCTGACTCGGGGACTCTGGAGAGGAAGCGACCTGCCAGCATGGCAGTGATGGAAGGG
GACTTGGTGAAGAAGGAGAGTCCTCCAAAACCCAAAGACTCCGTGTCTGCAGCTGTCCCTGCAGCAGGAA
GGAACAGCAACCAGATGACCACGGTCCCAAACCAGGCTCAGACAGGTGGCAACTCCCATCAGCTCTCAGT
AAGCACACCTCACAGTGCAGCCGGTCCCAGCCCGCACACTCTGCGTCGGGCTGTGAAGAAACCTGCCCCC
GCACCCCCCAAACCAGGAAACCTACCTCCTGGCCACCCTGGAGGACAGAGCTCTCCTGGCACAGGCACAT
CCCCAAAGCCAAGCGCCCGAAGCCCATCACCGCCTCAGCAGCAGCAACAACAGCAGCAACAACAGCAGCA
GCAGCAGACCCCAGGCATGCGCCGCTGCTCCAGCAGCCTGCCTCCCATCCAGGCACCCAGCCACCCACCA
CCACAGCCCCCCACACAGCCTCGGTTGGGTGAACAGGGACCAGAGCCAGGCCCCACGCCACCTCAAACCC
CCACACCACCTAGCACCCCACCCCTGGCCAAGCAGAATCCATCGCAAAGTGAGACCACACAGCTGCACGG
AACCCTCCCAAGACCACGGCCAGTGCCCAAGCCCCGCAACCGGCCTAGCGTGCCACCACCCCCACATCCA
CCTGGCACCCACACGGTGGATGGTGGCCTTACATCCTCAGTGCCCACAGCCTCCAGAATCGTCACTGATG
TA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210303 representing BC003259
Red=Cloning site Green=Tags(s)

MKKQFNRMKQLANQTVGRAEKTEVLSEDLLQIERRLDTVRSMCHHSHKRLIACFQGQHGTDAERRHKKLP
LTALAQNMQEASAQLEESLLGKMLETCGDAENQLALELSQHEVFVEKEIMDPLYGIAEVEIPNIQKQRKQ
LARLVLDWDSVRARWNQAHKSSGTNFQGLPSKIDTLKEEMDEAGNKVEQCKDQLAADMYNFMAKEGEYGK
FFVTLLEAQADYHRKALAVLEKALPEMRAHQDKWAEKPAFGTPLEEHLKRSGREIALPIEACVMLLLETG
MKEEGLFRIGAGASKLKKLKAALDCSTSHLDEFYSDPHAVAGALKSYLRELPEPLMTFSLYEEWTQVASV
QDQDKKLQYLWTTCQKLPPQNFVNFRYLIKFLAKLAQTSDVNKMTPSNIAIVLGPNLLWAKQEGTLAEIA
AATSVHVVAVIEPIIQHADWFFPGEVEFNVSEAFVPLATPNSNHSSHTGNDSDSGTLERKRPASMAVMEG
DLVKKESPPKPKDSVSAAVPAAGRNSNQMTTVPNQAQTGGNSHQLSVSTPHSAAGPSPHTLRRAVKKPAP
APPKPGNLPPGHPGGQSSPGTGTSPKPSARSPSPPQQQQQQQQQQQQQQTPGMRRCSSSLPPIQAPSHPP
PQPPTQPRLGEQGPEPGPTPPQTPTPPSTPPLAKQNPSQSETTQLHGTLPRPRPVPKPRNRPSVPPPPHP
PGTHTVDGGLTSSVPTASRIVTDV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC003259
ORF Size 2174 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC003259, AAH03259
RefSeq Size 3146 bp
RefSeq ORF 2174 bp
Locus ID 70497
Gene Summary Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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