Cd97 (BC006676) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AA409984; Cd97; TM7LN1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210300 representing BC006676 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGGCGTCAGATGCCCCGGCCTGCTTGTGGTGTGCATTTTACTGAGTCTCTCAGGAGCTGGAACCC AGAAGGCAGAGAGTAAGAACTGTGCCAAGTGGTGCCCTATCAACTCAAAATGTGTCAGTAACAGAAGCTG TGTCTGCAAGCCCGGCTTCAGCTCGGAGAAGGAATTGATCACTAACCCTGCAGAGAGCTGTGAAGACATC AACGAGTGTTTACTACCTGGATTTTCCTGTGGAGACTTTGCCATGTGTAAGAACTCAGAGGGGAGTTACA CCTGCGTCTGTAACCTGGGATATAAGCTCCTTTCTGGTGCAGAATCTTTTGTTAACGAGAGTGAGAATAC ATGTCAAGATGTGGATGAGTGCAGCTCTGGGCAGCATCAATGTCACAATTCCACCGTCTGCAAAAACACC GTAGGCTCCTACAAGTGCCACTGCCGTCCAGGTTGGAAGCCAACTTCTGGGTCCCTCCGTGGTCCAGACA CCATATGCCAAGAGCCACCGTTCCCTACTTGGACACTGCTGCCCACAGCCCACAGTCAGACTCTCTTGAG ATTCTCTGTTGAAGTCCAGAATCTGCTCCGTGACTTCAATCCAGCCACGGTCAACTACACCATCCAGAAA CTCATCGAGGCTGTGGACAAGCTGCTGGAAGACCCCATGGAGACTGAAACCCAACAAGTAGCTGCCCAGT TGCTCTCGAACCTGGAACAAAGCCTTCGGACCTTGGCTCAGTTTTTACCCAAAGGCCCCTTCACCTACAC GTCCCCTTCAAATACTGAACTGTCCCTGATGGTGAAGGAGCAAGACAACAAAGATGTTACCACAGTGCAC CACGGCCAAACTTGGATGGAGCTGGACTGGGCTGTGACAGCTGGAGCCAAAATCTCAGAAAATGGCTCCT CGGTGGCAGGCATCCTGTCCAGCCCAAACATGGAAAAGCTGCTGGGCAACACCCCGCTGAACTTGGAGCA GAGAAGGGCCTCCTTGGAGGACTTCTATGGAAGCCCGATTCCGAGTGTCTCACTTAAGCTTCTCTCAAAC ATCAATTCTGTCTTCCTGACCAACACGAACACTGAAAAGCTCGCCTCGAATGTCACGTTCAAGTTCGACT TTACTTCTGTGGAGTCAATTGAGCCACGTCATGAGCTGATATGTGCCTTCTGGAAAGCCCACAACGGGAA TGGGTACTGGGACACCGACGGCTGCTCAATGAATGGCACTGGCTTCTGCCACTGCAACCACCTGACCAGC TTTGCCATCCTAATGGCTCAGTACCATGTGCAGGACCCAAGGCTGGAATTGATCACCAAGGTGGGGCTGT TGTTGTCCCTGATCTGCCTGCTGCTGTGCATCCTGACCTTCCTGCTGGTGAAGCCCATCCAGAGCTCTCG AACCATGGTGCACCTGCACCTGTGCATCTGCCTCTTCCTGGGCTCAATCATATTCCTGGTCGGCGTGGAG AATGAAGGGGGTGAGGTGGGCCTGCGCTGCCGCCTGGTGGCCGTGATGCTGCATTTCTGCTTCCTGGCCG CCTTCTGCTGGATGGCACTGGAGGGCGTGGAGCTCTACTTCCTGGTGGTGCGTGTGTTCCAGGGCCAAGG CCTGAGTACATGGCAACGCTGCCTGATTGGCTACGGGGTGCCCCTCCTCATCGTGGCCATCTCAATGGCA GTTGTCAAAATGGATGGCTATGGGCATGCAACATACTGCTGGCTGGACTTTAGGAAGCAGGGCTTTCTCT GGAGCTTTTCGGGACCCGTGGCCTTCATCATTTTCTGCAATGCTGCCATTTTCGTGATCACTGTCTGGAA GCTCACAAAGAAGTTTTCTGAAATCAACCCAAACATGAAGAAGTTAAGGAAGGCGAGGGTGCTGACCATC ACCTCCATCGCCCAGCTCCTTGTGCTGGGCTGCACCTGGGGCTTCGGCCTGTTCCTCTTCAACCCCCATA GCACATGGCTATCCTACATCTTTACCCTGCTCAACTGCCTGCAGGGCCTATTCCTCTATGTGATGCTCTG CCTGCTCAACAAAAAGGTGAGGGAAGAGTACTGGAAATGGGCCTGCATGGTCACTGGGAGCAAGTACACA GAGTTCAACTCTTCCACAACAGGCACTGGCACCAGCCAAACACGGGCTCTCAGGTCCTCAGAATCAGGGA TG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210300 representing BC006676 Red=Cloning site Green=Tags(s) MRGVRCPGLLVVCILLSLSGAGTQKAESKNCAKWCPINSKCVSNRSCVCKPGFSSEKELITNPAESCEDI NECLLPGFSCGDFAMCKNSEGSYTCVCNLGYKLLSGAESFVNESENTCQDVDECSSGQHQCHNSTVCKNT VGSYKCHCRPGWKPTSGSLRGPDTICQEPPFPTWTLLPTAHSQTLLRFSVEVQNLLRDFNPATVNYTIQK LIEAVDKLLEDPMETETQQVAAQLLSNLEQSLRTLAQFLPKGPFTYTSPSNTELSLMVKEQDNKDVTTVH HGQTWMELDWAVTAGAKISENGSSVAGILSSPNMEKLLGNTPLNLEQRRASLEDFYGSPIPSVSLKLLSN INSVFLTNTNTEKLASNVTFKFDFTSVESIEPRHELICAFWKAHNGNGYWDTDGCSMNGTGFCHCNHLTS FAILMAQYHVQDPRLELITKVGLLLSLICLLLCILTFLLVKPIQSSRTMVHLHLCICLFLGSIIFLVGVE NEGGEVGLRCRLVAVMLHFCFLAAFCWMALEGVELYFLVVRVFQGQGLSTWQRCLIGYGVPLLIVAISMA VVKMDGYGHATYCWLDFRKQGFLWSFSGPVAFIIFCNAAIFVITVWKLTKKFSEINPNMKKLRKARVLTI TSIAQLLVLGCTWGFGLFLFNPHSTWLSYIFTLLNCLQGLFLYVMLCLLNKKVREEYWKWACMVTGSKYT EFNSSTTGTGTSQTRALRSSESGM TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC006676
ORF Size	2174 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC006676, AAH06676
RefSeq Size	2737 bp
RefSeq ORF	2174 bp
Locus ID	26364
Gene Summary	Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.[UniProtKB/Swiss-Prot Function]

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