Dlgh4 (BC014807) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	SAP90, PSD-95, SAP90A
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210275 representing BC014807 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTGTCTCTGTATAGTGACAACCAAGAAATACCGCTACCAAGATGAAGACACGCCCCCTCTGGAAC ACAGCCCGGCCCACCTCCCCAACCAGGCCAATTCTCCCCCTGTGATTGTCAACACGGACACCCTAGAAGC CCCAGGATATGTGAACGGAACAGAGGGGGAGATGGAGTATGAGGAGATCACATTGGAAAGGGGTAACTCA GGTCTGGGCTTCAGCATCGCAGGTGGCACCGACAACCCACACATCGGTGACGACCCATCCATCTTTATCA CCAAGATCATTCCTGGTGGGGCTGCAGCCCAGGATGGCCGCCTCAGGGTCAACGACAGCATCCTGTTTGT CAATGAAGTGGATGTCCGGGAGGTGACCCATTCAGCTGCAGTGGAGGCCCTCAAAGAGGCGGGTTCCATC GTTCGCCTCTACGTCATGCGCCGGAAACCCCCAGCTGAGAAGATCATAGAGATCAAGCTTATCAAAGGGC CTAAAGGACTTGGCTTCAGCATCGCAGGGGGCGTTGGGAACCAGCACATCCCTGGAGATAATAGCATCTA CGTAACCAAGATCATCGAAGGAGGCGCTGCCCACAAGGAGGGCAGGTTGCAGATCGGAGACAAGATCCTG GCGGTCAACAGTGTGGGGCTAGAGGATGTCATGCATGAGGACGCCGTGGCAGCCCTGAAGAACACATATG ACGTTGTGTACCTAAAGGTGGCCAAGCCCAGCAATGCCTACCTGAGTGACAGCTATGCTCCCCCAGACAT CACAACCTCATATTCTCAGCACCTGGACAATGAGATCAGTCATAGCAGCTACTTGGGCACCGACTACCCC ACAGCCATGACCCCCACTTCCCCTCGGCGCTACTCCCCCGTGGCCAAGGACCTGCTAGGGGAGGAAGATA TTCCCCGGGAACCAAGGCGGATCGTGATCCATCGGGGCTCCACCGGCCTGGGCTTCAACATTGTGGGCGG CGAGGACGGTGAAGGCATCTTCATCTCCTTCATCCTTGCTGGGGGTCCAGCTGACCTCAGTGGGGAGCTA CGGAAGGGGGACCAGATCCTGTCGGTCAATGGTGTTGACCTCCGCAATGCCAGTCATGAACAGGCTGCCA TTGCCCTGAAGAACGCGGGTCAGACGGTCACGATCATCGCTCAGTATAAACCAGAAGAGTATAGCCGATT CGAGGCCAAGATCCATGATCTTCGGGAACAGCTTATGAATAGTAGCCTGGGCTCAGGGACTGCATCTCTG CGAAGCAACCCCAAGCGGGGCTTCTATATCAGGGCCCTGTTTGACTACGACAAGACCAAGGACTGCGGTT TCTTGAGCCAGGCCCTGAGCTTCCACTTTGGGGATGTGCTTCATGTAATTGACGCCAGCGACGAAGAGTG GTGGCAAGCGCGGCGGGTCCACTCTGACAGTGAGACCGATGACATTGGCTTCATTCCCAGCAAACGGCGG GTCGAGCGACGAGAGTGGTCAAGGTTAAAGGCCAAGGACTGGGGCTCCAGCTCTGGATCACAGGGTCGAG AAGACTCGGTTCTGAGCTATGAGACGGTGACGCAGATGGAAGTGCACTACGCTCGCCCCATCATCATCCT TGGGCCTACCAAAGACCGTGCCAACGATGATCTTCTCTCCGAGTTCCCCGACAAGTTTGGATCCTGTGTC CCTCATACGACACGTCCTAAGCGGGAATATGAGATAGACGGCCGCGATTACCACTTTGTCTCCTCCCGGG AGAAAATGGAGAAGGACATTCAGGCGCACAAGTTCATTGAGGCTGGCCAGTACAACAGCCACCTCTACGG GACCAGCGTCCAGTCTGTGCGAGAGGTAGCAGAGCAGGGGAAGCACTGCATCCTTGATGTCTCAGCCAAT GCCGTGCGGCGGCTGCAGGCGGCCCACCTGCACCCTATCGCCATCTTCATCCGTCCCCGCTCCCTGGAGA ATGTGCTAGAGATCAATAAGCGGATCACAGAGGAGCAAGCCCGGAAAGCCTTCGACAGGGCCACGAAGCT GGAGCAGGAGTTCACGGAGTGCTTCTCAGCCATCGTAGAGGGCGACAGCTTTGAAGAGATCTATCACAAA GTGAAACGTGTCATCGAAGACCTCTCAGGCCCCTACATCTGGGTCCCAGCCCGAGAGAGACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210275 representing BC014807 Red=Cloning site Green=Tags(s) MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYVNGTEGEMEYEEITLERGNS GLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSI VRLYVMRRKPPAEKIIEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKEGRLQIGDKIL AVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYP TAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGEL RKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASL RSNPKRGFYIRALFDYDKTKDCGFLSQALSFHFGDVLHVIDASDEEWWQARRVHSDSETDDIGFIPSKRR VERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCV PHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSAN AVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHK VKRVIEDLSGPYIWVPARERL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC014807
ORF Size	2163 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC014807, AAH14807
RefSeq Size	3076 bp
RefSeq ORF	2165 bp
Locus ID	13385
Gene Summary	Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (Probable).[UniProtKB/Swiss-Prot Function]

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