Ilf3 (BC047272) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MPHOSPH4, NF90
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210237 representing BC047272 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATTGTATCATCATCACTTCATCACAAGAAGAAGAAGGCGTCCCATGAGAATTTTTGTGAATGACG ATCGCCACGTGATGGCAAAGCATTCTTCAGTGTACCCAACACAAGAGGAGCTGGAGGCTGTACAGAACAT GGTGTCCCATACTGAGCGGGCCCTGAAGGCTGTCTCTGACTGGATTGATGAGCAGGAGAAAGGCAACAGC GAGCTCTCTGAGGCAGAAAATATGGACACACCCCCAGACGATGAGAGCAAAGAAGGGGCTGGGGAACAGA AGGCGGAACACATGACTAGGACCCTGAGGGGCGTGATGCGGGTCGGCCTGGTAGCCAAGGGTCTTCTGCT CAAGGGGGACTTGGATCTGGAGCTGGTTCTGCTCTGTAAGGAGAAGCCCACAACCGCCCTTCTGGACAAG GTGGCTGACAACCTGGCCATCCAGCTCACTACTGTAACAGAAGACAAGTATGAAATACTCCAGTCTGTGG ATGATGCTGCGATTGTGATAAAAAACACAAAAGAGCCCCCCTTGTCCTTGACCATCCATCTGACCTCCCC TGTTGTCAGAGAAGAAATGGAGAAAGTATTAGCTGGAGAAACGCTATCAGTCAACGATCCCCCGGACGTT CTGGACAGGCAGAAATGCCTTGCTGCCTTGGCGTCCCTCCGACACGCCAAGTGGTTCCAGGCCAGAGCCA ATGGACTGAAGTCATGTGTCATTGTCATCCGTGTCCTAAGGGACTTGTGTACCCGAGTGCCCACCTGGGG TCCCCTCAGAGGATGGCCTCTGGAGCTGCTGTGTGAGAAGTCCATCGGCACTGCCAATAGGCCAATGGGT GCTGGTGAAGCCCTGCGGAGAGTGCTGGAGTGCCTGGCATCCGGCATCGTAATGCCAGATGGTTCTGGCA TTTATGACCCTTGTGAAAAAGAAGCCACTGATGCTATTGGGCATCTAGACAGACAGCAACGGGAAGATAT CACACAGAGTGCGCAGCATGCTCTGCGGCTTGCTGCCTTTGGTCAACTCCATAAAGTACTGGGAATGGAC CCCCTGCCTTCCAAAATGCCCAAGAAACCAAAGAACGAGAACCCGGTGGACTACACTGTTCAAATTCCTC CCAGCACCACCTATGCTATCACACCCATGAAACGCCCTATGGAAGAGGATGGGGAGGAGAAGTCTCCCAG CAAGAAGAAAAAGAAGATCCAGAAGAAAGAGGAGAAGGCTGATCCTCCTCAAGCTATGAATGCCCTGATG AGGTTAAATCAGCTGAAGCCAGGGCTGCAGTACAAGCTGATCTCCCAGACAGGCCCTGTTCATGCTCCCA TCTTCACCATGTCTGTGGAGGTAGATGGCAGTAACTTCGAGGCCTCGGGGCCATCTAAAAAGACTGCCAA GCTTCATGTAGCTGTGAAGGTGTTACAGGACATGGGCTTGCCAACAGGCGCTGAAGGCAGAGACTCCAGC AAGGGGGAAGACTCCGCTGAGGAGTCAGATGGGAAGCCAGCAATAGTGGCCCCACCCCCTGTGGTGGAAG CTGTCTCCAACCCCAGTTCTGTCTTCCCTTCAGATGCCACTACTGAGCAGGGACCGATTTTGACTAAGCA TGGCAAGAACCCTGTTATGGAGCTTAATGAGAAGAGACGTGGCCTCAAATATGAGCTCATTTCTGAGACG GGGGGCAGCCACGACAAAAGGTTTGTTATGGAGGTTGAGGTGGACGGACAGAAGTTTCAAGGTGCTGGTT CAAACAAAAAGGTGGCAAAGGCTTATGCTGCACTTGCCGCATTAGAAAAACTTTTCCCTGATACCCCTCT TGCTCTTGAAGCCAACAAAAAGAAAAGGACCCCAGTACCTGTCCGAGGTGGACCCAAATTTGCTGCCAAG CCACACAACCCTGGTTTTGGCATGGGAGGACCCATGCATAATGAAGTGCCGCCACCTCCTAACATCCGAG GTCGGGGCCGAGGAGGTAACATCCGAGGGCGAGGACGGGGGCGAGGATTTGGTGGCGCCAACCATGGAGG AGGCTACATGAATGCTGGTGCTGGATATGGAAGCTATGGGTACAGCAGCAATTCGGCCACAGCAGGCTAC AGTGACTTTTTCACAGACTGCTACGGCTATCATGATTTTGGGGCTTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210237 representing BC047272 Red=Cloning site Green=Tags(s) MALYHHHFITRRRRRPMRIFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGNS ELSEAENMDTPPDDESKEGAGEQKAEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDK VADNLAIQLTTVTEDKYEILQSVDDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDV LDRQKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWPLELLCEKSIGTANRPMG AGEALRRVLECLASGIVMPDGSGIYDPCEKEATDAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMD PLPSKMPKKPKNENPVDYTVQIPPSTTYAITPMKRPMEEDGEEKSPSKKKKKIQKKEEKADPPQAMNALM RLNQLKPGLQYKLISQTGPVHAPIFTMSVEVDGSNFEASGPSKKTAKLHVAVKVLQDMGLPTGAEGRDSS KGEDSAEESDGKPAIVAPPPVVEAVSNPSSVFPSDATTEQGPILTKHGKNPVMELNEKRRGLKYELISET GGSHDKRFVMEVEVDGQKFQGAGSNKKVAKAYAALAALEKLFPDTPLALEANKKKRTPVPVRGGPKFAAK PHNPGFGMGGPMHNEVPPPPNIRGRGRGGNIRGRGRGRGFGGANHGGGYMNAGAGYGSYGYSSNSATAGY SDFFTDCYGYHDFGAS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC047272
ORF Size	2150 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC047272, AAH47272
RefSeq Size	3705 bp
RefSeq ORF	2150 bp
Locus ID	16201
Gene Summary	The protein encoded by this gene contains two double-stranded RNA binding domains and functions in the post-transcriptional regulation of gene expression. It is a component of an RNA-protein complex that may be involved in mediating the export of messenger RNAs. Alternative splicing results in multiple transcript variants encoding distinct isoforms. These isoforms are grouped into two categories, NFAR-1 or NFAR-2, based on variation at the C-terminus. [provided by RefSeq, Mar 2013]

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