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Yme1l1 (NM_013771) Mouse Tagged ORF Clone
CAT＃: MG210226
- TrueORF®
Yme1l1 (tGFP-tagged) - Mouse YME1-like 1 (S. cerevisiae) (Yme1l1)
ORF Plasmid: DDK
"NM_013771" in other vectors (4)
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CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ftsh; FtsH1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210226 representing NM_013771
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCCCTGTCGAGCACTGTGCAGCCTCAGGTTACAATTCCTCTCAGTCATCTCATCAATGCTTTCC ATTCACCAAAAAATATATCTGTTTCTGTCAATACACCTGTTTCTCAAAAACAGCATCGAGATACAGTTCC TGAGCATGAAGCTCCCAGTAGTGAGCCTGTGCTTAATTTAAGGGACCTTGGATTATCTGAATTGAAAATT GGACAGATTGATAAAATGGTAGAAAATTTACTTCCTGGGTTTTATAAAGACAAAAGAGTTTCTTCCTGTT GGCATACATCTCATATTTCGGCACAGTCCTTTTTTGAAAATAAATATGGTCACTTAGATATGTTCAGTAC ATTACGTTCCTCTAGCTTGTACAGGCAACATCCAAAAACTCTTCGAAGCATTTGTTCAGATCTTCAGTAT TTTCCAGTTTTCATACAGTCTCGGGGTTTCAAAACGTTGAAATCAAGGACACGACGTTTGCAGTCTACCT CTGAAAGATTAGTAGAAGCACAGAATATAGCACCATCATTTGTGAAGGGGTTTCTTTTGCGGGACAGAGG AACAGATCTTGAGAGTTTGGACAAACTTATGAAAACTAAAAACATACCTGAAGCTCACCAAGATGCATTT AAAACTGGTTTTGCAGAGGGTTTTCTCAAAGCTCAAGCTCTTACACAGAAGACCAATGATTCCTTAAGGC GAACTCGTCTGATCCTCTTTGTTTTGCTCCTGTTTGGCATTTATGGACTCTTAAAAAATCCGTTTTTATC TGTGCGCTTCCGGACAACTACAGGACTTGATTCTGCGGTAGACCCTGTCCAGATGAAAAATGTCACTTTT GAACATGTTAAAGGGGTGGAGGAAGCCAAACAAGAGTTACAGGAAGTGGTTGAATTCTTGAAAAATCCAC AGAAGTTTACTGTGCTTGGAGGTAAACTTCCCAAAGGAATTCTTTTAGTTGGGCCACCAGGAACAGGGAA GACGCTTCTTGCCCGAGCTGTGGCAGGAGAAGCTGACGTTCCTTTTTATTATGCTTCTGGATCAGAGTTT GATGAGATGTTTGTGGGCGTAGGAGCCAGCAGGATCAGAAATCTTTTTAGAGAAGCAAAAGCAAATGCTC CTTGTGTTATATTCATCGATGAATTAGATTCTGTTGGTGGAAAGAGAATTGAATCTCCAATGCACCCGTA TTCAAGGCAGACGATCAATCAGCTTCTTGCTGAAATGGATGGTTTCAAACCCAATGAAGGAGTAATCATT ATAGGTGCCACAAATTTCCCAGAGGCATTAGATAATGCCTTAATACGTCCTGGTCGTTTTGATATGCAAG TTACAGTTCCAAGGCCAGATGTGAAGGGTCGAACTGAAATTTTGAAATGGTATCTCAACAAGATAAAGTT TGATAAATCTGTTGATCCAGAAATCATAGCTCGAGGGACTGTTGGGTTCTCTGGAGCAGAGTTGGAGAAT CTTGTGAACCAAGCTGCACTAAAGGCAGCAGTTGATGGAAAAGAAATGGTTACCATGAAGGAACTAGAGT TTTCCAAGGATAAAATTCTAATGGGGCCAGAAAGAAGAAGTGTGGAAATTGATAACAAAAACAAAACTAT AACAGCCTATCATGAATCTGGTCATGCTATTATTGCATATTACACAAAGGATGCAATGCCAATTAATAAA GCTACAATCATGCCTCGAGGGCCAACACTTGGGCATGTATCACTGTTGCCTGAGAATGACAGATGGAATG AAACTCGAGCCCAGCTGCTTGCACAGATGGATGTTAGCATGGGAGGAAGAGTGGCAGAGGAACTCATATT TGGAACTGATCATATTACAACTGGTGCTTCTAGTGATTTTGATAATGCAACAAAAATTGCTAAGAGGATG GTTACCAAATTTGGAATGAGTGAAAAGCTTGGAGTTATGACCTACAGTGATACAGGAAAACTAAGTCCTG AAACTCAATCAGCCATTGAACAAGAAATAAGAATCCTTCTACGGGAGTCATATGAACGAGCAAAACATAT CTTGAAAACACATGCGAAAGAACATAAGAACCTGGCAGAAGCATTGCTGACCTATGAGACTTTGGATGCC AAAGAGATTCAAATTGTTCTTGAGGGGAAGAAATTGGAAGTGAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210226 representing NM_013771
Red=Cloning site Green=Tags(s) MFSLSSTVQPQVTIPLSHLINAFHSPKNISVSVNTPVSQKQHRDTVPEHEAPSSEPVLNLRDLGLSELKI GQIDKMVENLLPGFYKDKRVSSCWHTSHISAQSFFENKYGHLDMFSTLRSSSLYRQHPKTLRSICSDLQY FPVFIQSRGFKTLKSRTRRLQSTSERLVEAQNIAPSFVKGFLLRDRGTDLESLDKLMKTKNIPEAHQDAF KTGFAEGFLKAQALTQKTNDSLRRTRLILFVLLLFGIYGLLKNPFLSVRFRTTTGLDSAVDPVQMKNVTF EHVKGVEEAKQELQEVVEFLKNPQKFTVLGGKLPKGILLVGPPGTGKTLLARAVAGEADVPFYYASGSEF DEMFVGVGASRIRNLFREAKANAPCVIFIDELDSVGGKRIESPMHPYSRQTINQLLAEMDGFKPNEGVII IGATNFPEALDNALIRPGRFDMQVTVPRPDVKGRTEILKWYLNKIKFDKSVDPEIIARGTVGFSGAELEN LVNQAALKAAVDGKEMVTMKELEFSKDKILMGPERRSVEIDNKNKTITAYHESGHAIIAYYTKDAMPINK ATIMPRGPTLGHVSLLPENDRWNETRAQLLAQMDVSMGGRVAEELIFGTDHITTGASSDFDNATKIAKRM VTKFGMSEKLGVMTYSDTGKLSPETQSAIEQEIRILLRESYERAKHILKTHAKEHKNLAEALLTYETLDA KEIQIVLEGKKLEVR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013771 |
| ORF Size | 2145 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013771.2, NP_038799.1 |
| RefSeq Size | 4571 bp |
| RefSeq ORF | 2148 bp |
| Locus ID | 27377 |
| UniProt ID | O88967 |
| Gene Summary | ATP-dependent metalloprotease that catalyzes the degradation of folded and unfolded proteins with a suitable degron sequence in the mitochondrial intermembrane region (By similarity). Plays an important role in regulating mitochondrial morphology and function by cleaving OPA1 at position S2, giving rise to a form of OPA1 that promotes maintenance of normal mitochondrial structure (PubMed:17709429, PubMed:24616225, PubMed:26785494, PubMed:27495975). Ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins (By similarity). Required for normal, constitutive degradation of PRELID1 (PubMed:26785494). Catalyzes the degradation of OMA1 in response to membrane depolarization. Required to control the accumulation of nonassembled respiratory chain subunits (NDUFB6, OX4 and ND1) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220981 | Yme1l1 (untagged) - Mouse YME1-like 1 (S. cerevisiae) (Yme1l1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 9,220.00 |
|
| MR210226 | Yme1l1 (Myc-DDK-tagged) - Mouse YME1-like 1 (S. cerevisiae) (Yme1l1), nuclear gene encoding mitochondrial protein |
CNY 5,248.00 |
|
| MR210226L3 | Lenti ORF clone of Yme1l1 (Myc-DDK-tagged) - Mouse YME1-like 1 (S. cerevisiae) (Yme1l1), nuclear gene encoding mitochondrial protein |
CNY 8,170.00 |
|
| MR210226L4 | Lenti ORF clone of Yme1l1 (mGFP-tagged) - Mouse YME1-like 1 (S. cerevisiae) (Yme1l1), nuclear gene encoding mitochondrial protein |
CNY 8,170.00 |
