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Ssrp1 (BC042502) Mouse Tagged ORF Clone
CAT＃: MG210205
- TrueORF®
Ssrp1 (tGFP-tagged) - Mouse structure specific recognition protein 1 (cDNA clone MGC:30412 IMAGE:5005209)
ORF Plasmid: DDK
"BC042502" in other vectors (4)
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CNY 4,470.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C81323; Hmg1-rs1; Hmgi-rs3; Hmgox; T160 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210205 representing BC042502
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGACATTGGAGTTCAACGACATCTTCCAGGAGGTGAAAGGGTCCATGAATGATGGGAGGCTTC GATTGAGCCGTCAGGGTATCATCTTTAAGAACAGCAAGACGGGCAAAGTGGACAACATCCAGGCTGGGGA GTTGACAGAAGGCATCTGGCGTCGGGTAGCATTAGGCCATGGGCTTAAACTGCTCACAAAGAATGGGCAT GTCTACAAGTACGATGGCTTCCGCGAATCGGAGTTTGAGAAACTCTCTGACTTCTTCAAAACTCACTATC GCCTTGAGCTAATGGAGAAGGATCTGTGTGTGAAGGGCTGGAACTGGGGGACAGTGAAGTTTGGAGGACA GCTGCTTTCTTTTGACATTGGTGATCAACCAGTCTTTGAGATACCCCTAAGCAATGTGTCCCAGTGTACC ACAGGCAAGAATGAGGTGACCCTGGAATTCCACCAGAATGACGATGCTGAAGTATCTCTCATGGAGGTGC GCTTCTATGTTCCTCCCACGCAGGAAGATGGTGTGGACCCTGTGGAGGCCTTTGCCCAGAATGTTCTGTC AAAGGCAGATGTGATCCAGGCCACCGGAGACGCCATCTGCATCTTCCGGGAGCTGCAGTGTTTGACTCCT CGCGGTCGATACGATATCCGGATCTACCCTACCTTTCTACACCTGCATGGCAAGACCTTTGACTACAAGA TCCCCTATACTACAGTTCTCCGTCTCTTCCTGCTACCACACAAGGATCAGAGACAGATGTTCTTTGTGAT CAGCTTGGATCCTCCCATCAAGCAGGGCCAAACTCGTTACCACTTCCTGATCCTCCTCTTCTCCAAGGAT GAGGACATCTCCTTGACTCTCAACATGAATGAGGAAGAAGTAGAAAAGCGCTTTGAGGGGCGACTCACCA AGAACATGTCAGGATCCCTCTATGAAATGGTCAGTCGGGTCATGAAAGCACTTGTCAACCGTAAAATCAC AGTCCCAGGCAACTTCCAAGGGCACTCAGGGGCCCAGTGTATTACCTGCTCCTATAAGGCCAGCTCAGGA CTCCTGTACCCACTGGAGCGGGGCTTCATCTACGTGCATAAGCCCCCTGTGCACATCCGCTTTGATGAGA TCTCTTTTGTCAACTTTGCCCGTGGCACCACGACCACTCGTTCCTTCGACTTTGAGATTGAGACCAAGCA AGGCACTCAGTATACCTTCAGCAGCATTGAAAGGGAGGAGTATGGAAAGCTTTTTGATTTTGTCAATGCG AAAAAGCTCAACATCAAGAACAGAGGACTGAAAGAGGGCATTAACCCAGGCTATGACGATTATGCTGACT CTGATGAAGACCAGCATGATGCCTATTTGGAGAGGATGAAGGAGGAGGGCAAGATCCGGGAGGAGAATGC CAATGACAGCAGCGACGACTCAGGAGAAGAGACTGATGAGTCCTTCAATCCTGGTGAAGAAGAAGAAGAT GTGGCAGAGGAGTTTGACAGCAATGCCTCTGCCAGCTCCTCCAGCAATGAGGGTGACAGTGACCGTGAAG AGAAGAAACGGGAACAGCTCAAAAGGGCTAAGATGGCCAAGGATCGAAAGAGCCGCAGGAAGTCTTCAGA GGCAAAGAAGGGTAAAGATCCAAACGCCCCAAAGAGGCCTATGTCTGCGTACATGCTGTGGCTTAATGCA AGCCGCGAGAAGATCAAGTCGGATCATCCTGGCATCAGTATCACAGATCTTTCCAAGAAGGCAGGGGAAA TCTGGAAGGGAATGTCCAAAGAGAAGAAAGAGGAGTGGGACCGCAAGGCTGAGGATGCTAGGAGGGAGTA TGAGAAAGCCATGAAAGAGTATGAAGGAGGCAGAGGGGACTCGTCTAAAAGGGACAAGTCTAAGAGGGAC AAGTCTAAGAAGAAAAAGAAAGTAAAAGCAAAGATGGAAAAAAAGTCCACTCCTTCCCGGGGCTCGTCAT CCAAGTCTTCATCCAGGCAGTTGAGTGACAGCTTCAAGAGCAAAGAGTTTGTGTCCAGTGATGAGAGCTC TTCAGGCGAGAACAAGAGCAAAAAGAAGAGGAGGCGGAGCGAGGACTCTGAAGAGGAGCTAGCCAGTACC CCCTCCAAGCTCAGAGGACTCTGCCTCGGGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210205 representing BC042502
Red=Cloning site Green=Tags(s) MAETLEFNDIFQEVKGSMNDGRLRLSRQGIIFKNSKTGKVDNIQAGELTEGIWRRVALGHGLKLLTKNGH VYKYDGFRESEFEKLSDFFKTHYRLELMEKDLCVKGWNWGTVKFGGQLLSFDIGDQPVFEIPLSNVSQCT TGKNEVTLEFHQNDDAEVSLMEVRFYVPPTQEDGVDPVEAFAQNVLSKADVIQATGDAICIFRELQCLTP RGRYDIRIYPTFLHLHGKTFDYKIPYTTVLRLFLLPHKDQRQMFFVISLDPPIKQGQTRYHFLILLFSKD EDISLTLNMNEEEVEKRFEGRLTKNMSGSLYEMVSRVMKALVNRKITVPGNFQGHSGAQCITCSYKASSG LLYPLERGFIYVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNA KKLNIKNRGLKEGINPGYDDYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEED VAEEFDSNASASSSSNEGDSDREEKKREQLKRAKMAKDRKSRRKSSEAKKGKDPNAPKRPMSAYMLWLNA SREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARREYEKAMKEYEGGRGDSSKRDKSKRD KSKKKKKVKAKMEKKSTPSRGSSSKSSSRQLSDSFKSKEFVSSDESSSGENKSKKKRRRSEDSEEELAST PSKLRGLCLGI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC042502 |
| ORF Size | 2133 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC042502.1 |
| RefSeq Size | 2792 bp |
| RefSeq ORF | 2135 bp |
| Locus ID | 20833 |
| Gene Summary | Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA. Also acts as a transcriptional coactivator for p63/TP63.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220760 | Ssrp1 (untagged) - Mouse structure specific recognition protein 1 (cDNA clone MGC:30412 IMAGE:5005209), (10ug) |
CNY 9,120.00 |
|
| MR210205 | Ssrp1 (Myc-DDK-tagged) - Mouse structure specific recognition protein 1 (cDNA clone MGC:30412 IMAGE:5005209) |
CNY 5,208.00 |
|
| MR210205L3 | Lenti ORF clone of Ssrp1 (Myc-DDK-tagged) - Mouse structure specific recognition protein 1 (cDNA clone MGC:30412 IMAGE:5005209) |
CNY 5,990.00 |
|
| MR210205L4 | Lenti ORF clone of Ssrp1 (mGFP-tagged) - Mouse structure specific recognition protein 1 (cDNA clone MGC:30412 IMAGE:5005209) |
CNY 5,990.00 |
