Mipep (BC110358) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	5730405E07Rik; MIP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210203 representing BC110358 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGGCGGCCGGGGCACGGTACGCCCGCCGGCTGTGCGGCCGGGGAGCCGCGGCGGCTCTGCAGG GACGCACAGGCCGGAGCTGCGCCCGGGACGTCAGTACTAGCTGGTCCCCGGTGGGCGCCGCCTTCAACGT CAAGCCCCAGAGCCACCTATGGAACCTGTTGGGGGAGCGCCGGGGTCTTTTTGGAGTTCCTGAGCTCAGC ACCCCTGAAGGATTCCAAGTTGCCCAAGAGGAAGCCTTGAAGAAGACGGAGTGGCTGGTAGAGCGAGCAT GTTCCACACCCCCAGGGCCACAGACAGTCCTCATTTTTGACGAACTCTCGGATTGCCTGTGCAGAGTGGC TGACTTGGCGGACTTTGTGAAAATTGGACACCCAGACCCTGCATTCAGAGAGGCTGCGCAAGAGGCCTGC AGAAGTATTGGCACCATGGTGGAAAAGTTGAATACAAACGTGGAGCTGTATCAGAGTCTGCAAAGGTTAC TGGGTGATAAGAAGCTTATGGAGTCCCTTGATGCGGAAACCAGGCGAGTGGCCGAACTATTTATGTTTGA TTTTGAAATCAGTGGAATCCATCTAGATGAAGAAAAGCGCAGAAGAGCAGTGGACCTCAATGTTAAGATC TTGGACTTGAGCAATGCTTTCCTCATGAGAACCAACTTTCCCAACAAGATCCGGAAGAGTCTCTTACCAG AGCACATTCAGCACCACTTTGCCCGTGACGGAAGCCACTTAATCATTGACGGCCTGCATGCAGAAGCCTC CGATGACTTGGTTCGGGAGGCTGCTTATAAAATTTTCCTTTATCCCAATGCCGATCAGTTGAAATGTTTA GAGGAATTGCTAAGCAGCAGAGACCTTCTGGCAAAATTAGTAGGTTATTCTACATTTTCCCACAGGGCCC TCCAAGGAACCATAGCTCAGACTCCAGAGACTGTCATGCAGTTCCTTGAGAAGCTATCTGAAAAGCTCTC TGAAAGAACTAGAAAAGATTTCAAGATGATGCAAGGAATGAAAACAAAACTAAATCCTCAAAATTCTAAA CTAATGCCTTGGGACCCTCCCTACTACAGCGGTGTGATTCGTGCAGAGAGGTATAATATTGAGCCCAGTT TATACTGCCCGTTTTTATCCCTTGGAGCTTGCATGGAAGGCCTGAATGTCTTATTTAACAAACTGCTGGG CATCACCTTATATGCAGAGCAGACCTTCAAAGGAGAGGTGTGGTGTAATGACATCCGGAAGCTGGCTGTT GTTCATGAATCTGAAGGGCTGCTGGGGTACATTTACTGTGATTTTTTCCAGAGAGCAAACAAGCCACAGC AGGATTGCCACTTCACCATCCGAGGAGGCAGATTAAAGGAAGATGGCAGCTATCAGCTTCCAGTGGTCGT TCTCATGCTGAATCTTCCTCATGCTTCCAGGGATTTCCCTACATTATTAACTCCAGGAATGATGGAAAAT CTCTTCCATGAAATGGGCCATGCCATGCATTCAATGCTTGGACGAACTCGGCACCAACATGTCACTGGGA CCAGGTGCCCTACTGACTTTGCTGAAGTTCCATCTATTCTGATGGAGTACTTTTCAAATGATTACCGAGT AGTCAGCCAGTTCGCCAAGCATTATCAGACTGGTCAGCCACTCCCGAAAGCTATGGTGTCTCGTCTCTGT GAGTCTAAGAAGGTTTGCACAGCAGCAGAAATGCAGCTTCAGGTCTTTTATGCCGCCCTGGATCAAATCT ACCATGGCCAGCATCCCCTGAAGAAGTCAACCACAGATATCCTCATGGAAACACAAGAGCAGTTCTATGG CTTGCCCTATGTTCCTGACACTGCCTGGCAGCTGAGGTTCAGCCACCTCGTGGGATATGGTGCCAAGTAT TACTCATACCTGATGTCCAGGGCTGTGGCATCCATGATCTGGAAGGAGTGCTTCCTACAGGATCCTTTTA ACAGGGCCGCTGGGGAACGCTACCGCAGAGAGATGTTGGCCCATGGTGGAGGCAAGGAGCCCATGCTTAT GATTCAAGGCATGCTGCAGAAGTGTCCTTCCATCGATGACTTTGTGGATGCCCTCGTGTCTGACATGAAT CTGGACTTTGAAACGTTCTTCCTGGATTCTAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210203 representing BC110358 Red=Cloning site Green=Tags(s) MLLAAGARYARRLCGRGAAAALQGRTGRSCARDVSTSWSPVGAAFNVKPQSHLWNLLGERRGLFGVPELS TPEGFQVAQEEALKKTEWLVERACSTPPGPQTVLIFDELSDCLCRVADLADFVKIGHPDPAFREAAQEAC RSIGTMVEKLNTNVELYQSLQRLLGDKKLMESLDAETRRVAELFMFDFEISGIHLDEEKRRRAVDLNVKI LDLSNAFLMRTNFPNKIRKSLLPEHIQHHFARDGSHLIIDGLHAEASDDLVREAAYKIFLYPNADQLKCL EELLSSRDLLAKLVGYSTFSHRALQGTIAQTPETVMQFLEKLSEKLSERTRKDFKMMQGMKTKLNPQNSK LMPWDPPYYSGVIRAERYNIEPSLYCPFLSLGACMEGLNVLFNKLLGITLYAEQTFKGEVWCNDIRKLAV VHESEGLLGYIYCDFFQRANKPQQDCHFTIRGGRLKEDGSYQLPVVVLMLNLPHASRDFPTLLTPGMMEN LFHEMGHAMHSMLGRTRHQHVTGTRCPTDFAEVPSILMEYFSNDYRVVSQFAKHYQTGQPLPKAMVSRLC ESKKVCTAAEMQLQVFYAALDQIYHGQHPLKKSTTDILMETQEQFYGLPYVPDTAWQLRFSHLVGYGAKY YSYLMSRAVASMIWKECFLQDPFNRAAGERYRREMLAHGGGKEPMLMIQGMLQKCPSIDDFVDALVSDMN LDFETFFLDSK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC110358
ORF Size	2135 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC110358, AAI10359
RefSeq Size	2401 bp
RefSeq ORF	2135 bp
Locus ID	70478
Gene Summary	Cleaves proteins, imported into the mitochondrion, to their mature size.[UniProtKB/Swiss-Prot Function]

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