Cnot4 (BC058778) Mouse Tagged ORF Clone
CAT#: MG210197
- TrueORF®
Cnot4 (tGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 4 (cDNA clone MGC:65243 IMAGE:5397231)
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CNY 6,940.00
货期*
4周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Not4h, Not4hp |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210197 representing BC058778
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCGCAGTCCTGATGCAAAGGAAGACCCTGTGGAATGCCCTCTTTGCATGGAGCCCTTGGAAATAG ATGATATTAACTTTTTCCCTTGCACCTGTGGCTACCAGATATGCCGATTTTGTTGGCATCGAATTCGCAC TGATGAGAATGGGCTTTGTCCTGCATGTAGAAAGCCATATCCAGAAGATCCAGCAGTTTACAAACCACTT TCCCAGGAAGAATTACAAAGGATAAAGAATGAGAAAAAACAGAAACAAAATGAAAGAAAACAGAAAATCT CAGAAAATAGAAAACATTTGGCTAGTGTACGTGTTGTACAAAAAAACCTTGTGTTTGTTGTAGGTCTGTC ACAGCGCCTAGCAGACCCAGAAGTTTTAAAACGACCAGAGTATTTTGGGAAGTTTGGTAAAATACATAAA GTTGTCATCAATAATAGCACATCCTATGCAGGCTCACAGGGTCCGAGTGCCAGTGCTTATGTAACGTATA TCCGGTCAGAAGATGCTCTCAGAGCAATACAGTGTGTCAACAATGTGGTAGTAGATGGCAGGACACTAAA GGCATCTTTAGGTACAACAAAGTACTGCAGTTATTTCTTAAAGAACATGCAGTGCCCAAAGCCTGACTGC ATGTATCTTCATGAATTGGGAGATGAGGCAGCCAGCTTCACAAAAGAGGAAATGCAGGCGGGTAAGCACC AAGAATATGAACAGAAGCTCCTCCAAGAATTATATAAATTAAATCCCAATTTTCTTCAGCTATCTACGGG TTCAGTTGATAAGAATAAGAACAAAGTGACACCATTACAAAGCCCCATTGACAAACCTTCAGATTCTCTC AGTATAGGGAATGGTGATAATTCCCAACAGATATCTAACAGTGATACGCCTTCACCACCACCTGGTTTAT CAAAATCCAACCCTGTCATCCCCATCAGTTCATCCAATCACAGTGCGAGGTCTCCTTTTGAAGGGGCAGT AACAGAGTCACAGTCATTATTCTCAGACAATTTTCGCCACCCCAACCCTATACCAAGTGGGCTTCCTCCT TTTCCCAGCTCTCCACAGACACCCAGTGATTGGCCTACAGCTCCAGAACCACAGAGCCTCTTCACATCAG AAACAATCCCAGTTTCATCATCTACAGACTGGCAAGCAGCATTCGGCTTTGGTTCTTCTAAACAACCAGA GGATGACTTGGGGTTTGACCCCTTTGATGTCACTCGAAAAGCCTTAGCAGACCTGATTGAGAAGGAACTA TCCGTCCAAGATCAACCTTCCCTTTCCCCCACATCTCTTCAGAACGCCTCCTCACACACTACGACCGCCA AAGGGCCAGGCTCTGGATTCCTGCACTCTGCTGCACCTACAAATGCCAACTCTCTCAATAGTACCTTTTC AGTCTTGCCACAGAGGTTCCCTCAATTTCAGCAGCACCGAGCAGTTTATAATTCGTTCGGTTTTCCAGGC CAGGCAGCCCGCTATCCTTGGATGGCCTTTCCACGCAATAGCATCATGCACTTGAACCACACAGCAAACC CCACCTCAAATAGTAATTTCTTGGACTTGAATCTCCCGCCACAGCACAACACAGGTCTGGGAGGGATCCC CATAGCAGACAACAACAGTTCTGTAGAGAGTTTAAATATGAAGGAATGGCAGGACGGGCTAAGGGCACTT CTACCCAACATTAACATCAACTTTGGTGGACTGCCCAATTCTTCTTCCCCCTCCAACGCCAACCACAGTG CACCAACGTCCAACACTGCCACCACGGACAGCGTGAGTTGGGACAGCCCTGGCAGCTGGACAGACCCAGC CATCATCACAGGTATTCCAGCATCTTCAGGAAACACTTTAGACTCTATTCAAGACGACAATCCTCCACAC TGGCTAAAATCCCTACAGGCCCTCACAGAGATGGACGGCCCCAGCGCTGCCTCGTCACAACCCCATCACA GCGCCCCCTTCAGCACACAGATCCCTCTGCACAGAGCCAGTTGGAACCCCTACCCACCTCCTTCAAATCC CTCCAGCTTCCACTCTCCACCCCCAGGCTTCCAGACAGCCTTCAGACCCCCCAGCAAAACCCCCACAGAT CTGCTACAGAGTTCCACACTGGACCGCCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210197 representing BC058778
Red=Cloning site Green=Tags(s) MSRSPDAKEDPVECPLCMEPLEIDDINFFPCTCGYQICRFCWHRIRTDENGLCPACRKPYPEDPAVYKPL SQEELQRIKNEKKQKQNERKQKISENRKHLASVRVVQKNLVFVVGLSQRLADPEVLKRPEYFGKFGKIHK VVINNSTSYAGSQGPSASAYVTYIRSEDALRAIQCVNNVVVDGRTLKASLGTTKYCSYFLKNMQCPKPDC MYLHELGDEAASFTKEEMQAGKHQEYEQKLLQELYKLNPNFLQLSTGSVDKNKNKVTPLQSPIDKPSDSL SIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDNFRHPNPIPSGLPP FPSSPQTPSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIEKEL SVQDQPSLSPTSLQNASSHTTTAKGPGSGFLHSAAPTNANSLNSTFSVLPQRFPQFQQHRAVYNSFGFPG QAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPIADNNSSVESLNMKEWQDGLRAL LPNININFGGLPNSSSPSNANHSAPTSNTATTDSVSWDSPGSWTDPAIITGIPASSGNTLDSIQDDNPPH WLKSLQALTEMDGPSAASSQPHHSAPFSTQIPLHRASWNPYPPPSNPSSFHSPPPGFQTAFRPPSKTPTD LLQSSTLDRH TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC058778 |
ORF Size | 2132 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC058778, AAH58778 |
RefSeq Size | 3486 bp |
RefSeq ORF | 2132 bp |
Locus ID | 53621 |
Gene Summary | Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins. Involved in activation of the JAK/STAT pathway. Catalyzes ubiquitination of methylated RBM15.[UniProtKB/Swiss-Prot Function] |
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