Ngef (BC039279) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	ephexin; Tims2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210191 representing BC039279 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCAAAAACTCTGAAGACTGGGGGAAACCTCAGAGGAAATCAGAGAGCAGCTCACGGAAAAGCA ATCATGGCCCAGCTGAGATGAGGCCTGCGTTACCTCCGGAGAACAGGGAGGCACCTGAGACTGGCGAGGA AACTCAAAACGAAGAACCCCGCCGCCTCATCCCAATCCAGAGGCATTCCCTCTTCAATCGTGCTGTGAGA CACAGGCACAAGGCCAGGAGCACGTCTGAGCGGAGAGCCAGTGACCAAGCAGATTTGCCAAAAATGGGGA AATCTGTAAATGAACGGTCGGCCTTCAATCTGCCCCAGGGGAGATTGTCTCCCTGGAGAACACCAGCCCA GAGGGACACAGGAGCGCAGGAAGCCAGCGAGTCATCCTCCACCCCTGGAAATGGAACCACACCCGAGGAG TGCCCAGCCCTCACCGACAGCCCCACCACTCTCACGGAGGCCCTGCAGATGATCCATCCCATTCCCGCCG ACTCCTGGAGAAACCTCATTGAACAAATAGGGCTCCTGTATCAAGAGTATAGAGACAAATCGACTCTCCA AGAAATTGAAACACGGAGGCAGCAGGATGCAGAAATCCAAGGCAACTCCGATGGGTCCCAGGTTGGGGAA GACGCTGGAGAGGAGGAGGAGGAGGAGGAGGAGGGAGAGGAGGAGGAGCTGGCCAGCCCTCCTGAGAGGA GAGCTCTGCCTCAGATCTGCCTGCTCAGCAACCCCCACTCCAGGTTCAACCTCTGGCAAGACCTTCCTGA GATCCAGAGCAGTGGCGTGCTGGACATTCTCCAGCCGGAGGAGATCAGGCTGCAGGAGGCCATGTTTGAG TTGGTTACCTCTGAGGCCTCCTACTATAAGAGCCTGAACCTGCTGGTGTCGCACTTCATGGAGAACGAGC GTCTGAAGAAGATCCTGCATCCATCCGAGGCCCACATCCTCTTTTCCAATGTCCTGGATGTCATGGCTGT CAGTGAGCGGTTTTTGCTGGAGCTAGAGCACCGCATGGAGGAGAACATTGTTATCTCGGATGTGTGCGAC ATCGTGTACCGTTACGCAGCTGATCACTTCTCGGTCTATATCACTTACGTCAGTAACCAGACCTACCAGG AAAGGACATACAAGCAGCTCCTACAGGAGAAGGCCGCTTTCCGGGAACTGATCGCACAGTTGGAGCTGGA CCCCAAATGCAAGGGCCTGCCTTTCTCCTCCTTCCTCATCTTGCCTTTCCAGAGGATCACGAGACTCAAG CTGCTGGTCCAGAATATCCTGAAGAGAGTGGAGGAGAGGTCTGAACGTGAAGGCACCGCCTTGGATGCCC ACAAGGAGCTAGAAATGGTGGTAAAGGCATGCAATGAGGGTGTCCGGAAGATGAGCCGCACAGAACAGAT GATCAGCATTCAGAAGAAGATGGAGTTCAAGATCAAGTCGGTACCCATCATCTCACACTCCCGGTGGCTG CTGAAGCAGGGTGAGCTGCAGCAGATGTCCGGCCCCAAGACCTCCCGCACCCTGCGGACCAAGAAGCTCT TCAGAGAAATTTACCTCTTCCTCTTCAATGACCTGCTGGTGATCTGCCGGCAGATCCCTGGAGACAAGTA CCAGGTGTTTGATTCGGCCCCAAGGGGCCTGCTTCGAGTGGAGGAGCTGGAGGACCAGGGTCAAACACTG GCTAATGTGTTCATCCTGCGGCTGCTGGAAAATGCAGATGACCGAGAGGCCACCTATATGCTGAAGGCAT CCTCCCAGAGCGAGATGAAGCGCTGGATGACCTCACTGGCCCCCAACAGGAGGACCAAGTTTGTATCCTT CACATCTCGGCTGTTGGACTGTCCCCAGGTCCAGTGTGTGCACCCGTATGTGGCCCAGCAGCCTGATGAA CTGACGCTGGAACTGGCAGATATCCTGAACATCCTGGAGAAGACAGAGGATGGGTGGATCTTTGGTGAGC GGCTGCATGACCAGGAGAGAGGCTGGTTCCCCAGTTCCATGACAGAGGAGATCCTGAACCCCAAGATCCG CTCCCAGAACCTCAAGGAATGTTTCCGGGTACATAAGATGGAAGACCCTCAGCGCAGCCAGAATAAGGAC CGCAGGAAGCTGGGCAGCCGGAATCGTCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210191 representing BC039279 Red=Cloning site Green=Tags(s) METKNSEDWGKPQRKSESSSRKSNHGPAEMRPALPPENREAPETGEETQNEEPRRLIPIQRHSLFNRAVR HRHKARSTSERRASDQADLPKMGKSVNERSAFNLPQGRLSPWRTPAQRDTGAQEASESSSTPGNGTTPEE CPALTDSPTTLTEALQMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIQGNSDGSQVGE DAGEEEEEEEEGEEEELASPPERRALPQICLLSNPHSRFNLWQDLPEIQSSGVLDILQPEEIRLQEAMFE LVTSEASYYKSLNLLVSHFMENERLKKILHPSEAHILFSNVLDVMAVSERFLLELEHRMEENIVISDVCD IVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCKGLPFSSFLILPFQRITRLK LLVQNILKRVEERSEREGTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWL LKQGELQQMSGPKTSRTLRTKKLFREIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTL ANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDE LTLELADILNILEKTEDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMEDPQRSQNKD RRKLGSRNRQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC039279
ORF Size	2132 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC039279, AAH39279
RefSeq Size	3154 bp
RefSeq ORF	2132 bp
Locus ID	53972
Gene Summary	Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.[UniProtKB/Swiss-Prot Function]

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