Acsl6 (BC016114) Mouse Tagged ORF Clone

CAT#: MG210102

  • TrueORF®

Acsl6 (tGFP-tagged) - Mouse acyl-CoA synthetase long-chain family member 6 (cDNA clone MGC:28744 IMAGE:4481949)



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Mouse Protein / Lysate Request

CNY 6,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms MGC36650, LACS, mKIAA0837
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210102 representing BC016114
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGACCCAGGAGATCCTGAGGATCCTGCGGCTGCCCGAGCTATCGGACTTGGGCCAGTTTTTCCGCA
GCCTCTCAGCTACCACCCTCGTGAGTGTGGGTGCACTGGCTGCCGTCCTTGCCTACTGGCTCACTCACCG
GCCAAAGGCCTTGCAACCACCATGCAACCTCCTGAAGCAGTCGGAAGAAGTGGAGGACGGTGGTGGAGCC
CGGCGATCTGTGATTGGGGGTTGCACTCAATTGCTTACCCATTACTATGACGATGCCCGGACCATGTACC
AGGTGTTCCGCCGTGGGCTTAGCATCTCAGGGAATGGACCCTGTCTTGGCTTCCGGAAGCCAGAGCAACC
TTACCAGTGGCTGTCCTACCAAGAGGTGGCCAAAAGGGCTGAATTTCTGGGGTCGGGGCTTCTCCAGCAC
GACTGTAAAGTGGGCACAGAGCAGTTCGTTGGTGTTTTTGCACAAAATCGGCCGGAGTGGATCATTGCCG
AGCTGGCCTGCTACACGTATTCCATGGTGGTGGTACCGCTCTACGACACCCTGGGCCCAGGGTCTATCAG
CTACATCATCAATACTGCGGACATTTGCACGGTAATCGTCGATAAACCCCACAAGGCAACACTTCTGCTG
GAACACGTGGAGAGGAAGGAGACTCCGGGCCTCAAGCTGGTCATCCTCATGGAGCCGTTTGAGGACGCCC
TGAGAGAGAGAGGAAAGAAGTGCGGGGTGGACATCAAGTCCATGCAGGCTATAGAGGACTGTGGCCGAGA
GAATCATCATGCCCCCGTGCCCCCACGGCCTGATGACCTCTCCATCGTGTGTTTCACAAGTGGTACAACA
GGGAACCCCAAAGGTGCAATGCTCACCCACGGGAACGTGGTGGCCGATTTCTCGGGCTTTCTGAAAGTGA
CAGAGAAAGTGATCTTTCCGAGACAGGACGATGTGCTCATCTCCTTCCTGCCTCTGGCTCACATGTTTGA
GAGAGTGATCCAGTCTGTTGTCTACTGCCACGGAGGGCGCGTGGGCTTCTTCCAGGGAGACATCCGCCTC
CTCTCAGATGACATGAAGGCTCTCCGCCCTACCATCTTCCCTGTGGTCCCACGGCTGCTGAATCGGATGT
ATGACAAGATCTTCCACCAGGCAGACACCTCACTAAAGCGCTGGCTCCTGGAGTTTGCAGCAAAGCGCAA
GCAGGCAGAGGTCCGGAGCGGAATCATCAGAAACAATAGTATCTGGGATGAACTCTTCTTTAATAAGATT
CAGGCCAGTCTTGGTGGGCATGTGAGGATGATTGTCACTGGAGCAGCCCCCGCGTCACCAACGGTTCTGG
GATTCCTACGAGCAGCTCTGGGGTGCCAGGTCTATGAAGGTTATGGGCAAACTGAATGCACAGCTGGGTG
TACCTTCACAACGCCAGGGGACTGGACATCAGGGCATGTAGGGGCACCTCTGCCCTGCAACCACATCAAG
CTGGTCGATGCAGAGGAACTCAACTACTGGACCTGCAAAGGAGAAGGAGAGATATGTGTGAAAGGACCAA
ATGTGTTCAAAGGCTACTTAAAAGACGAGGACAGGACAAAGGAGGCCCTGGACAGCGACGGCTGGCTTCA
CACTGGAGACATTGGGAAATGGCTGCCGGAGGGAACACTCAAAATCATTGATCGGAAAAAGCACATATTT
AAACTTGCTCAGGGGGAATATGTTGCGCCAGAGAAGATCGAGAACATCTACATCCGGAGTGAGCCTGTGG
CACAAATCTACGTCCACGGGGACAGCTTAAAGGCCTTTTTGGTTGGCATTGTCGTGCCTGACCCTGAAGT
CATGCCTTCCTGGGCTCAGAAGAAAGGAATCGAAGGGACCTATCAGGAACTCTGCATGAAAAAGGAATTG
AAGAAAGCCATTCTGGATGACATGGTGATGCTGGGGAAAGAAAGCGGACTGCATTCTTTTGAACAGGTTA
AAGCCATTTACATCCATTGTGACATGTTCTCTGTTCAAAATGGTCTGCTGACACCAACACTAAAGGCTAA
GAGACCGGAGCTGAGAGAGTACTTCAAAAAGCAAATAGAAGAGCTTTACTTAGTCTCCGTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210102 representing BC016114
Red=Cloning site Green=Tags(s)

MQTQEILRILRLPELSDLGQFFRSLSATTLVSVGALAAVLAYWLTHRPKALQPPCNLLKQSEEVEDGGGA
RRSVIGGCTQLLTHYYDDARTMYQVFRRGLSISGNGPCLGFRKPEQPYQWLSYQEVAKRAEFLGSGLLQH
DCKVGTEQFVGVFAQNRPEWIIAELACYTYSMVVVPLYDTLGPGSISYIINTADICTVIVDKPHKATLLL
EHVERKETPGLKLVILMEPFEDALRERGKKCGVDIKSMQAIEDCGRENHHAPVPPRPDDLSIVCFTSGTT
GNPKGAMLTHGNVVADFSGFLKVTEKVIFPRQDDVLISFLPLAHMFERVIQSVVYCHGGRVGFFQGDIRL
LSDDMKALRPTIFPVVPRLLNRMYDKIFHQADTSLKRWLLEFAAKRKQAEVRSGIIRNNSIWDELFFNKI
QASLGGHVRMIVTGAAPASPTVLGFLRAALGCQVYEGYGQTECTAGCTFTTPGDWTSGHVGAPLPCNHIK
LVDAEELNYWTCKGEGEICVKGPNVFKGYLKDEDRTKEALDSDGWLHTGDIGKWLPEGTLKIIDRKKHIF
KLAQGEYVAPEKIENIYIRSEPVAQIYVHGDSLKAFLVGIVVPDPEVMPSWAQKKGIEGTYQELCMKKEL
KKAILDDMVMLGKESGLHSFEQVKAIYIHCDMFSVQNGLLTPTLKAKRPELREYFKKQIEELYLVSV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC016114
ORF Size 2093 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC016114, AAH16114
RefSeq Size 2513 bp
RefSeq ORF 2093 bp
Locus ID 216739
Gene Summary Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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