Acsl6 (BC016114) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC36650, LACS, mKIAA0837
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210102 representing BC016114 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGACCCAGGAGATCCTGAGGATCCTGCGGCTGCCCGAGCTATCGGACTTGGGCCAGTTTTTCCGCA GCCTCTCAGCTACCACCCTCGTGAGTGTGGGTGCACTGGCTGCCGTCCTTGCCTACTGGCTCACTCACCG GCCAAAGGCCTTGCAACCACCATGCAACCTCCTGAAGCAGTCGGAAGAAGTGGAGGACGGTGGTGGAGCC CGGCGATCTGTGATTGGGGGTTGCACTCAATTGCTTACCCATTACTATGACGATGCCCGGACCATGTACC AGGTGTTCCGCCGTGGGCTTAGCATCTCAGGGAATGGACCCTGTCTTGGCTTCCGGAAGCCAGAGCAACC TTACCAGTGGCTGTCCTACCAAGAGGTGGCCAAAAGGGCTGAATTTCTGGGGTCGGGGCTTCTCCAGCAC GACTGTAAAGTGGGCACAGAGCAGTTCGTTGGTGTTTTTGCACAAAATCGGCCGGAGTGGATCATTGCCG AGCTGGCCTGCTACACGTATTCCATGGTGGTGGTACCGCTCTACGACACCCTGGGCCCAGGGTCTATCAG CTACATCATCAATACTGCGGACATTTGCACGGTAATCGTCGATAAACCCCACAAGGCAACACTTCTGCTG GAACACGTGGAGAGGAAGGAGACTCCGGGCCTCAAGCTGGTCATCCTCATGGAGCCGTTTGAGGACGCCC TGAGAGAGAGAGGAAAGAAGTGCGGGGTGGACATCAAGTCCATGCAGGCTATAGAGGACTGTGGCCGAGA GAATCATCATGCCCCCGTGCCCCCACGGCCTGATGACCTCTCCATCGTGTGTTTCACAAGTGGTACAACA GGGAACCCCAAAGGTGCAATGCTCACCCACGGGAACGTGGTGGCCGATTTCTCGGGCTTTCTGAAAGTGA CAGAGAAAGTGATCTTTCCGAGACAGGACGATGTGCTCATCTCCTTCCTGCCTCTGGCTCACATGTTTGA GAGAGTGATCCAGTCTGTTGTCTACTGCCACGGAGGGCGCGTGGGCTTCTTCCAGGGAGACATCCGCCTC CTCTCAGATGACATGAAGGCTCTCCGCCCTACCATCTTCCCTGTGGTCCCACGGCTGCTGAATCGGATGT ATGACAAGATCTTCCACCAGGCAGACACCTCACTAAAGCGCTGGCTCCTGGAGTTTGCAGCAAAGCGCAA GCAGGCAGAGGTCCGGAGCGGAATCATCAGAAACAATAGTATCTGGGATGAACTCTTCTTTAATAAGATT CAGGCCAGTCTTGGTGGGCATGTGAGGATGATTGTCACTGGAGCAGCCCCCGCGTCACCAACGGTTCTGG GATTCCTACGAGCAGCTCTGGGGTGCCAGGTCTATGAAGGTTATGGGCAAACTGAATGCACAGCTGGGTG TACCTTCACAACGCCAGGGGACTGGACATCAGGGCATGTAGGGGCACCTCTGCCCTGCAACCACATCAAG CTGGTCGATGCAGAGGAACTCAACTACTGGACCTGCAAAGGAGAAGGAGAGATATGTGTGAAAGGACCAA ATGTGTTCAAAGGCTACTTAAAAGACGAGGACAGGACAAAGGAGGCCCTGGACAGCGACGGCTGGCTTCA CACTGGAGACATTGGGAAATGGCTGCCGGAGGGAACACTCAAAATCATTGATCGGAAAAAGCACATATTT AAACTTGCTCAGGGGGAATATGTTGCGCCAGAGAAGATCGAGAACATCTACATCCGGAGTGAGCCTGTGG CACAAATCTACGTCCACGGGGACAGCTTAAAGGCCTTTTTGGTTGGCATTGTCGTGCCTGACCCTGAAGT CATGCCTTCCTGGGCTCAGAAGAAAGGAATCGAAGGGACCTATCAGGAACTCTGCATGAAAAAGGAATTG AAGAAAGCCATTCTGGATGACATGGTGATGCTGGGGAAAGAAAGCGGACTGCATTCTTTTGAACAGGTTA AAGCCATTTACATCCATTGTGACATGTTCTCTGTTCAAAATGGTCTGCTGACACCAACACTAAAGGCTAA GAGACCGGAGCTGAGAGAGTACTTCAAAAAGCAAATAGAAGAGCTTTACTTAGTCTCCGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210102 representing BC016114 Red=Cloning site Green=Tags(s) MQTQEILRILRLPELSDLGQFFRSLSATTLVSVGALAAVLAYWLTHRPKALQPPCNLLKQSEEVEDGGGA RRSVIGGCTQLLTHYYDDARTMYQVFRRGLSISGNGPCLGFRKPEQPYQWLSYQEVAKRAEFLGSGLLQH DCKVGTEQFVGVFAQNRPEWIIAELACYTYSMVVVPLYDTLGPGSISYIINTADICTVIVDKPHKATLLL EHVERKETPGLKLVILMEPFEDALRERGKKCGVDIKSMQAIEDCGRENHHAPVPPRPDDLSIVCFTSGTT GNPKGAMLTHGNVVADFSGFLKVTEKVIFPRQDDVLISFLPLAHMFERVIQSVVYCHGGRVGFFQGDIRL LSDDMKALRPTIFPVVPRLLNRMYDKIFHQADTSLKRWLLEFAAKRKQAEVRSGIIRNNSIWDELFFNKI QASLGGHVRMIVTGAAPASPTVLGFLRAALGCQVYEGYGQTECTAGCTFTTPGDWTSGHVGAPLPCNHIK LVDAEELNYWTCKGEGEICVKGPNVFKGYLKDEDRTKEALDSDGWLHTGDIGKWLPEGTLKIIDRKKHIF KLAQGEYVAPEKIENIYIRSEPVAQIYVHGDSLKAFLVGIVVPDPEVMPSWAQKKGIEGTYQELCMKKEL KKAILDDMVMLGKESGLHSFEQVKAIYIHCDMFSVQNGLLTPTLKAKRPELREYFKKQIEELYLVSV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC016114
ORF Size	2093 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016114, AAH16114
RefSeq Size	2513 bp
RefSeq ORF	2093 bp
Locus ID	216739
Gene Summary	Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid (By similarity).[UniProtKB/Swiss-Prot Function]

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