Stim1 (BC021644) Mouse Tagged ORF Clone

CAT#: MG210034

  • TrueORF®

Stim1 (tGFP-tagged) - Mouse stromal interaction molecule 1 (cDNA clone MGC:13964 IMAGE:4191156)



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Mouse Protein / Lysate Request

CNY 6,560.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms SIM
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210034 representing BC021644
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGTGTGCGCCCGTCTTGCCCTGTGGCTTCTTTGGGGGCTCCTTCTGCATCAGGGCCAGAGTCTCA
GCCATAGTCACAGTGAAAAGAATACAGGAGCTAGCTCCGGGGCGACTTCTGAAGAGTCTACCGAAGCAGA
GTTTTGCCGAATTGACAAGCCCCTGTGCCACAGTGAGGATGAGAAGCTCAGCTTTGAGGCCGTCCGAAAC
ATCCATAAGCTGATGGATGACGATGCCAATGGTGATGTGGATGTGGAAGAAAGTGATGAGTTCCTAAGGG
AAGACCTCAATTACCATGACCCAACAGTGAAACATAGCACCTTCCATGGTGAGGATAAGCTTATCAGCGT
GGAGGACCTGTGGAAGGCGTGGAAATCATCAGAAGTGTACAACTGGACTGTGGATGAGGTGATACAGTGG
CTCATTACGTATGTGGAGCTGCCACAGTATGAGGAGACCTTCCGGAAGTTGCAGCTTACTGGCCACGCCA
TGCCAAGGCTAGCAGTAACCAACACCACCATGACAGGGACTGTACTGAAGATGACAGATCGGAGCCACAG
GCAGAAGCTGCAGCTGAAGGCCCTGGACACAGTGCTGTTTGGGCCTCCTCTCTTGACTCGGCATAATCAC
CTGAAGGACTTCATGCTGGTGGTGTCTATCGTTATTGGTGTGGGTGGCTGCTGGTTTGCCTATATCCAGA
ACCGTTACTCTAAGGAGCACATGAAGAAAATGATGAAGGATCTGGAAGGGTTACACCGGGCTGAGCAGAG
TCTGCATGACCTTCAGGAAAGGCTGCACAAGGCCCAGGAGGAGCACCGAACTGTGGAAGTAGAGAAAGTC
CACCTGGAGAAGAAGCTGCGAGATGAGATCAACCTTGCCAAGCAGGAAGCTCAGCGGCTGAAGGAGCTGA
GGGAGGGTACTGAGAATGAGAGGAGCCGTCAAAAATATGCTGAGGAAGAGCTGGAGCAGGTTCGGGAGGC
CTTGAGGAAAGCAGAGAAGGAGCTGGAATCACACAGCTCATGGTATGCTCCTGAGGCCCTGCAGAAGTGG
CTGCAGCTGACCCATGAGGTGGAGGTGCAGTACTACAACATCAAGAAGCAAAATGCAGAGAGGCAGCTGC
TGGTGGCCAAGGAGGGGGCTGAGAAAATAAAAAAGAAGAGAAACACGCTTTTTGGTACCTTCCATGTGGC
CCACAGCTCTTCCCTGGATGATGTGGATCATAAAATCCTAACTGCTAAGCAAGCTCTGAGTGAGGTGACA
GCGGCACTGAGGGAGCGCCTGCACCGGTGGCAGCAGATCGAGATCCTCTGCGGTTTCCAGATTGTCAATA
ACCCCGGCATCCACTCCTTGGTGGCTGCTCTCAACATCGACCCCAGCTGGATGGGCAGCACCCGCCCTAA
CCCCGCCCACTTCATCATGACTGACGATGTGGATGACATGGATGAGGAGATTGTGTCGCCCTTGTCCATG
CAGTCCCCCAGCCTGCAGAGCAGTGTCCGGCAGCGCCTGACGGAGCCACAGCTTGGCCTGGGATCTCAGA
GGGATTTGACCCATTCCGATTCGGAGTCCTCCCTCCACATGAGTGACCGCCAGCGTGTGGCCCCCAAGCC
TCCTCAGATGGGCCGTGCTGCAGATGAAGCTCTCAATGCCATGCCTTCCAATGGCAGCCATCGGCTGATT
GAGGGGGTCCATCCAGGATCTCTGGTGGAGAAACTGCCTGACAGCCCTGCTCTGGCCAAGAAGACATTTA
TGGCGTTGAACCATGGCCTAGACAAGGCCCACAGCCTGATGGAGCTGAACCCCTCAGTCCCACCTGGTGG
CTCCCCACTTTTGGATTCTTCCCATTCTCTTAGCCCCAGTTCCCCAGACCCAGACACACCATCTCCAGTT
GGGGACAACCGAGCTCTGCAGGGTAGCCGAAACACACGAATTCCCCACTTGGCTGGCAAGAAGGCAATGG
CTGAGGAGGATAATGGTTCCATTGGTGAGGAGACAGACTCCAGTCCAGGCAGGAAGAAGTTTCCTCTCAA
AATTTTTAAGAAGCCTCTTAAGAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210034 representing BC021644
Red=Cloning site Green=Tags(s)

MDVCARLALWLLWGLLLHQGQSLSHSHSEKNTGASSGATSEESTEAEFCRIDKPLCHSEDEKLSFEAVRN
IHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQW
LITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNH
LKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKV
HLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKW
LQLTHEVEVQYYNIKKQNAERQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVT
AALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSM
QSPSLQSSVRQRLTEPQLGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQMGRAADEALNAMPSNGSHRLI
EGVHPGSLVEKLPDSPALAKKTFMALNHGLDKAHSLMELNPSVPPGGSPLLDSSHSLSPSSPDPDTPSPV
GDNRALQGSRNTRIPHLAGKKAMAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC021644
ORF Size 2057 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC021644, AAH21644
RefSeq Size 3619 bp
RefSeq ORF 2057 bp
Locus ID 20866
Gene Summary Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit ORAI1. Involved in enamel formation. Activated following interaction with STIMATE, leading to promote STIM1 conformational switch.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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