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Pelp1 (BC016444) Mouse Tagged ORF Clone
CAT＃: MG209953
- TrueORF®
Pelp1 (tGFP-tagged) - Mouse proline, glutamic acid and leucine rich protein 1 (cDNA clone MGC:25365 IMAGE:4459875)
ORF Plasmid: DDK
"BC016444" in other vectors (4)
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CNY 4,280.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RP23-42P20.4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209953 representing BC016444
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCAGGGAGGAGCCTCTGGAGAAGCCTTGCTCACCCACCTGCTCAGTGACATCTCCCCTCCAGCTG ATGCTCTTAAGCTATGCAGTACCAGGGGAAGCTCTGATGGAGGTTTGCAAAGTGGGAAGCCAAGTGCTCC TAAGAAGCTCAAGCTCGATATGGGGGAAGCTTTGGCTCCACCCAGCCAAAGGAAAGGAGATAGGAATGCC AACAGTGACGTGTGCGCAGCTGCACTGAGAGGCCTCAGTCGGACCATCCTTATGTGCGGGCCTCTCATCA AGGAGGAGACTCACAGGAGACTTCATGATCTAGTCCTGCCCCTGGTCATGAGTGTCCAGCAGGGTGAGGT CCCGGGCAGCTCTCCTTACAACAGTTCCTGCTGTCGCCTCGGGCTCTACCGCCTGCTGCTGGCTTTATTG CTGGCACCTTCCCCTCGGTGCCCTCCTCCTCTTGCCTGTGCCCTGAAAGCTTTCTCTCTTGGCCAATGGG AAGATAGTCTTGAGGTCTCCTCTTTTTGCTCAGAAGCTCTGGTGACCTGTGCTGCTCTGACACACCCCCG AGTACCTCCTCTACAGAGCTCAGGCCCTGCCTGCCCCACTCCTGCCCCCGTCCCTCCTCCTGAGGCCCCA TCTTCATTCAGGGCCCCGGCCTTCCATCCTCCAGGCCCCATGCCCTCCATAGGTGCTGTGCCATCCACAG GCCCATTACCCTCAGCAGGCCCCATTCCCACAGTAGGCTCTATGGCGTCCACAGGCCAAGTGCCCTCTCG CCCTGGACCTCCAGCAACAGCCAACCATTTAGGCCTCTCTGTCCCAGGCCTGGTATCTGTTCCACCCAGG CTCCTTCCTGGCCCTGAAAACCACCGTGCAGGCTCCGGTGAGGATCCTGTCCTTGCTCCTAGTGGGACCC CTCCGCCTAGCATACCTCCAGATGAAACTTTTGGGGGGAGAGTACCTAGGCCAGCCTTTGTCCACTACGA TAAGGAGGAGGCATCTGATGTAGAGATCTCCTTGGAAAGCGACTCTGATGACAGTGTGGTGATTGTACCT GAGGGGCTTCCCTCCCTGCCACCTGCACCACCCTCAGGCACTCCTCCCCCTGCGGCTCCTGCCGGCCCAC CAACAGCCTCACCTCCTGTGCCAGCTAAGGAGGATTCTGAAGAACTGCCTGCAACCCCAGGGCCTCCACC CCCACCCCCACCCCCACCCCCACCGGCCTCTGGTCCTGTGACACTTCCTCCACCCCAGCTGGTCCCTGAA GGGACTCCTGGAGGGGGAGGACCCACAGCCATGGAAGAAGATTTGACAGTTATTAATATCAACAGCAGTG ATGAAGAGGAGGAGGAGGAAGAGGAAGAGGAGGAAGAAGATGAAGATGAGGAAGAAGAAGACTTTGAGGA AGAGGAAGAAGATGAAGAAGAATATTTTGAAGAGGAAGAAGAGGAAGAAGAGTTTGAGGAGGAATTTGAG GAGGAAGAAGGTGAATTAGAAGAGGAAGAAGAAGAGGAGGAAGAAGAGTTAGATGAGGTAGAAGATGTGG AGTTTGGTTCAGCAGGGGAGGTGGAAGAAGGTGGACCTCCACCCCCAACACTGCCACCTGCTCTGCCACC TTCAGACTCGCCCAAAGTACAGCCTGAGGCAGAACCTGAACCTGGGCTCTTATTGGAAGTTGAGGAGCCA GGGCCAGAGGAGGTGCCTGGGCCTGAGACTGCTCCTACCCTGGCCCCTGAAGTGCTCCCCTCCCAGGAGG AGGGGGAGCAGGAAGTGGGAAGCCCTGCAGCAGGGCCACCTCAGGAGCTTGTTGAAGAGTCCTCTGCTCC CCCTGCCCTGCTGGAAGAGGGGACTGAGGGTGGAGGTGACAAAGTGCCACCCCCACCGGAGACACCTGCA GAAGAAATGGAGACAGAAGCAGAAGTTCCAGCTCCTCAGGAAAAGGAGCAAGATGATACAGCTGCCATGC TGGCTGATTTCATCGATTGTCCCCCTGACGATGAGAAGCCACCACCTGCTACAGAGCCTGACTCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209953 representing BC016444
Red=Cloning site Green=Tags(s) MLQGGASGEALLTHLLSDISPPADALKLCSTRGSSDGGLQSGKPSAPKKLKLDMGEALAPPSQRKGDRNA NSDVCAAALRGLSRTILMCGPLIKEETHRRLHDLVLPLVMSVQQGEVPGSSPYNSSCCRLGLYRLLLALL LAPSPRCPPPLACALKAFSLGQWEDSLEVSSFCSEALVTCAALTHPRVPPLQSSGPACPTPAPVPPPEAP SSFRAPAFHPPGPMPSIGAVPSTGPLPSAGPIPTVGSMASTGQVPSRPGPPATANHLGLSVPGLVSVPPR LLPGPENHRAGSGEDPVLAPSGTPPPSIPPDETFGGRVPRPAFVHYDKEEASDVEISLESDSDDSVVIVP EGLPSLPPAPPSGTPPPAAPAGPPTASPPVPAKEDSEELPATPGPPPPPPPPPPPASGPVTLPPPQLVPE GTPGGGGPTAMEEDLTVININSSDEEEEEEEEEEEEDEDEEEEDFEEEEEDEEEYFEEEEEEEEFEEEFE EEEGELEEEEEEEEEELDEVEDVEFGSAGEVEEGGPPPPTLPPALPPSDSPKVQPEAEPEPGLLLEVEEP GPEEVPGPETAPTLAPEVLPSQEEGEQEVGSPAAGPPQELVEESSAPPALLEEGTEGGGDKVPPPPETPA EEMETEAEVPAPQEKEQDDTAAMLADFIDCPPDDEKPPPATEPDS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC016444 |
| ORF Size | 2027 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC016444, AAH16444 |
| RefSeq Size | 2272 bp |
| RefSeq ORF | 2027 bp |
| Locus ID | 75273 |
| Gene Summary | Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Involved in nuclear receptor signaling via its interaction with AR and NR3C1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K (By similarity). Functions as the key stabilizing component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (PubMed:22872859). Component of the PELP1 complex involved in the nucleolar steps of 28S rRNA maturation and the subsequent nucleoplasmic transit of the pre-60S ribosomal subunit. Regulates pre-60S association of the critical remodeling factor MDN1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220842 | Pelp1 (untagged) - Mouse proline, glutamic acid and leucine rich protein 1 (cDNA clone MGC:25365 IMAGE:4459875), (10ug) |
CNY 9,120.00 |
|
| MR209953 | Pelp1 (Myc-DDK-tagged) - Mouse proline, glutamic acid and leucine rich protein 1 (cDNA clone MGC:25365 IMAGE:4459875) |
CNY 4,944.00 |
|
| MR209953L3 | Lenti ORF clone of Pelp1 (Myc-DDK-tagged) - Mouse proline, glutamic acid and leucine rich protein 1 (cDNA clone MGC:25365 IMAGE:4459875) |
CNY 5,800.00 |
|
| MR209953L4 | Lenti ORF clone of Pelp1 (mGFP-tagged) - Mouse proline, glutamic acid and leucine rich protein 1 (cDNA clone MGC:25365 IMAGE:4459875) |
CNY 5,800.00 |
