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Gpsm2 (NM_029522) Mouse Tagged ORF Clone
CAT＃: MG209933
- TrueORF®
Gpsm2 (tGFP-tagged) - Mouse G-protein signalling modulator 2 (AGS3-like, C
ORF Plasmid: DDK
"NM_029522" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6230410J09Rik; LGN; Pins |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209933 representing NM_029522
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGAAGACCATTCCTTTCATGTCCGCTACAGAATGGAAGCTTCTTGCCTTGAGCTGGCCTTGGAAG GAGAACGTCTATGTAAATCAGGGGACTGCCGCGCTGGCGTGTCATTTTTTGAAGCCGCAGTTCAAGTTGG AACTGAAGACCTCAAAACACTCAGTGCTATTTACAGCCAACTGGGCAATGCTTACTTCTACTTGCATGAC TACGCCAAAGCGTTAGAATACCACCACCATGACTTAACTCTTGCACGGACTATTGGAGACCAACTTGGGG AAGCTAAAGCCAGCGGCAATCTCGGAAACACCTTAAAGGTGCTTGGGAATTTTGATGAAGCGATAGTGTG TTGCCAGCGACACCTGGATATCTCCAGAGAGCTTAATGACAAGGTGGGAGAAGCAAGAGCGCTCTACAAT CTTGGAAATGTCTATCACGCCAAAGGGAAAAGCTTTGGTTGCCCGGGTCCGCAGGATACAGGAGAGTTTC CAGAAGACGTGAGAAATGCCCTACAGGCAGCTGTGGATCTCTATGAGGAAAACCTGTCCTTAGTAACTGC TCTAGGTGACCGAGCAGCACAGGGGCGAGCCTTTGGGAACCTTGGCAACACACACTACCTCCTCGGCAAC TTCAGGGATGCAGTTATAGCTCACGAGCAGCGTCTCCTAATTGCAAAGGAGTTCGGAGATAAGGCGGCTG AGAGAAGAGCGTACAGCAACCTAGGAAATGCATACATATTCCTGGGTGAATTCGAAACTGCTTCCGAATA CTACAAGAAGACACTGCTGCTGGCTCGGCAGCTTAAAGACAGAGCCGTGGAAGCTCAGTCTTGCTACAGT CTTGGGAATACATACACTTTGCTTCAGGACTACGAGAAGGCCATCGATTACCACCTAAAGCACTTAGCAA TCGCCCAAGAGCTCAAGGATCGGATTGGTGAAGGAAGAGCGTGTTGGAGCTTAGGAAACGCATACACAGC TCTGGGAAATCATGACCAAGCAATGCATTTTGCTGAAAAGCACTTGGAAATTTCAAGAGAGGTTGGGGAC AAAAGTGGTGAACTGACTGCGCGGCTGAACCTCTCAGACCTGCAGATGGTTCTTGGTCTGAGCTACAGCA CAAATAATTCCATGATGTCTGAGAACATTGAAATTGATGGCAGCTTACATGGTGCAGGCGCCAAGCTGGG ACGGAGACACAGTATGGAGAACTTGGAACTTATGAAGTTAACACCAGAAAAGGTGCCGAATTGGAACAGT GAAATTCTCGCTAAACAGAAACCTCTCATTGCCAAACCATCTGCAAAGCTCCTGTTTGTCAACAGACTGA AGGGCAAAAAGTACAAAAGCGGCAGCGCCTGCACTAAGGTTCTCCAAGACGCCAGCAACTCCGTGGACCA CCGCGCGCCACGCTCCCAGAAGAAAATCAGTTCAGACACGATTGGAGATGAAGGATTCTTTGACTTGCTA AGGCGATTTCAGAGCAACAGGATGGATGACCAGAGGTGCCACTTACAAGGAAACTGTCGCACAACATCCA CAGCAGCTGCTTCTGCTACCCCCAAGTTGATGAAAGCACCGTCTGTCTCTGTGGTGTCCCCCAACACAGA TGAGTTCTTAGATCTTCTTGCTAGCTCACAGAGCCGCCGTCTGGATGACCAGAGGGCCAGTTTCAGTAAT TTGCCAGGGCTCCGCCTGACAAAAGGCAACAGTCCATCTGTACTTGAGCGCCTGATGACAAATGACAAGA AAGAGCCTGATGAAGACTTCTTCGACATCCTTGTAAAGTGCCAGGGGTCAAGATTAGATGATCAAAGATG TGCTCCTCCATCTGCTGCCACTAAGGGGCCGACTGTCCCGGATGAGGACTTCTTTAGCCTCATCTTACGC TCTCAAGCTAAAAGAATGGATGAGCAGAGAGTTCTGCTGCAAAGAGATCCAAACAGAGACAGTGAGTTTG GACTAAAGGAACTTTTGCAAAATAACGCTTTGTTGGAATTTAAGCATTCGGGAAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209933 representing NM_029522
Red=Cloning site Green=Tags(s) MREDHSFHVRYRMEASCLELALEGERLCKSGDCRAGVSFFEAAVQVGTEDLKTLSAIYSQLGNAYFYLHD YAKALEYHHHDLTLARTIGDQLGEAKASGNLGNTLKVLGNFDEAIVCCQRHLDISRELNDKVGEARALYN LGNVYHAKGKSFGCPGPQDTGEFPEDVRNALQAAVDLYEENLSLVTALGDRAAQGRAFGNLGNTHYLLGN FRDAVIAHEQRLLIAKEFGDKAAERRAYSNLGNAYIFLGEFETASEYYKKTLLLARQLKDRAVEAQSCYS LGNTYTLLQDYEKAIDYHLKHLAIAQELKDRIGEGRACWSLGNAYTALGNHDQAMHFAEKHLEISREVGD KSGELTARLNLSDLQMVLGLSYSTNNSMMSENIEIDGSLHGAGAKLGRRHSMENLELMKLTPEKVPNWNS EILAKQKPLIAKPSAKLLFVNRLKGKKYKSGSACTKVLQDASNSVDHRAPRSQKKISSDTIGDEGFFDLL RRFQSNRMDDQRCHLQGNCRTTSTAAASATPKLMKAPSVSVVSPNTDEFLDLLASSQSRRLDDQRASFSN LPGLRLTKGNSPSVLERLMTNDKKEPDEDFFDILVKCQGSRLDDQRCAPPSAATKGPTVPDEDFFSLILR SQAKRMDEQRVLLQRDPNRDSEFGLKELLQNNALLEFKHSGK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029522 |
| ORF Size | 2016 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029522.1, NP_083798.1 |
| RefSeq Size | 3354 bp |
| RefSeq ORF | 2040 bp |
| Locus ID | 76123 |
| UniProt ID | Q8VDU0 |
| Gene Summary | Plays an important role in mitotic spindle pole organization via its interaction with NUMA1 (PubMed:21816348). Required for cortical dynein-dynactin complex recruitment during metaphase (By similarity). Plays a role in metaphase spindle orientation (By similarity). Plays an important role in asymmetric cell divisions (PubMed:12571286, PubMed:21816348). Has guanine nucleotide dissociation inhibitor (GDI) activity towards G(i) alpha proteins, such as GNAI1 and GNAI3, and thereby regulates their activity (PubMed:22952234).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205426 | Gpsm2 (untagged) - Mouse G-protein signalling modulator 2 (AGS3-like, C. elegans) (Gpsm2), (10ug) |
CNY 4,928.00 |
|
| MR209933 | Gpsm2 (Myc-DDK-tagged) - Mouse G-protein signalling modulator 2 (AGS3-like, C. elegans) (Gpsm2) |
CNY 4,928.00 |
|
| MR209933L3 | Lenti ORF clone of Gpsm2 (Myc-DDK-tagged) - Mouse G-protein signalling modulator 2 (AGS3-like, C. elegans) (Gpsm2) |
CNY 7,700.00 |
|
| MR209933L4 | Lenti ORF clone of Gpsm2 (mGFP-tagged) - Mouse G-protein signalling modulator 2 (AGS3-like, C. elegans) (Gpsm2) |
CNY 7,700.00 |
