5730410E15Rik (BC065045) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Golsyn, mKIAA1472
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209873 representing BC065045 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCCCTCCGAGAGAGCAAGGAGCAGAGAGTGCAGCATCAGGAGAAAGAGATTTCCAGGAGCCGGA TTCCCCGGTTGATTCTGCGGCCCCATCGGCCTCAGCAGCAGCAACAGCAGCAGAACAAGGTTTCCCCAGC CTCCGAGTCTCCCTTTTCGGAGGAAGAAAGTAGAGAGTTCAACCCCAGCAGCTCTGGACGCTCAGCAAGG ACGATTAGCAGCAACAGCTTCTGCTCAGATGACACAGGTTGTCCCAGCAGCCAGTCGGTATCGCCTGTGA AGACTCCTTCAGACACAGGGCACAGTCCCATTGGCTTTTGCCCTGGAAGCGATGAAGACTTTACCAGGAA GAAATGCAGGATTGGAATGGTTGGTGAGGGAAGTATTCAGTCAGCTCGTCATAAAAAAGAACCCAAGGGA GGCATCATAAAGCCAGGCAGTGAAGCAGATTTTAGCTCCTCGAGCAGCACAGGCAGCATCTCGGCTCCTG AGGTCCACATGTCCACGACAGGAAACAAGAGAGCCTCTTTCTCACGCAACCGAGGCCCTCATGGGCGGAG CAATGGAGCATCATCCCACAAGTCTGGCAGCAGCCCACCATCCCCAAGGGAAAAAGACCTTGTGTCTATG CTGTGCAGGAATCCACTGAGCCCCAGTAACATCCACCCTAGTTACGCCCCTTCTTCTCCAAGTAGCAGCA ACTCCGGCTCCTACAAAGGAAGTGATTGTAGTCCAGTCATGAGAAGGTCTGGAAGATACATGTCTTGTGG CGAAAATCACGGTGTCAAACCCCCAAATCCAGAACAGTATTTGACTCCTCTGCAGCAGAAGGAGGTCACA GTGAGGCACCTGAGGACCAAGCTGAAGGAGTCTGAGCGCCGACTCCATGAGAGGGAATCTGAAATCATGG AGCTCAAGTCTCAGCTGGCTCGAATGAGGGAGGACTGGATTGAGGAAGAGTGCCACAGAGTGGAGGCTCA GTTGGCACTCAAAGAAGCCAGAAAAGAGATTAAACAGCTCAAACAGGTCATCGAGACTATGAGGAGCAGC TTGGCTGATAAAGATAAAGGCATTCAGAAGTACTTTGTGGACATAAACATCCAAAACAAGAAACTGGAGT CTCTGCTTCAAAGCATGGAGATGGCACACAATAGTTCCCTGAGGGATGAACTGTGTCTTGACTTTTCCTT TGATTCCCCAGAGAAAAGTTTACCCCTGAGCAGCACATTTGACAAGTTGCCAGATGGGTTATCTCTGGAA GAACAGATAACAGAGGAAGGTGCTGACAGTGAGCTTCTGGTGGGAGACAGCATGGCTGAAGGCACAGATC TGTTAGATGAGATGGTGACTGCCACCACCACAGAATCCAGTGGCCTGGAGTTTGTTCATTCCACTCCAGG GCCACAAGCCCTCAAGGCTCTCCCCTTGGTGAGCCACGAAGAGGGCATTGCGGTGATGGAGCAAGCCGTG CAGACCGACGTGGTGCCGTTCAGCCCTGCCATCTCAGAGCTCATTCAGAGTGTGCTAAAGCTGCAGGACT ACTGTCCCACAAGCTCAGCATCTCCAGATGAATCTGGAGCTGACTCGATGGAAAGCTTCTCAGAATCTAT CTCTGCCTTAATGCTTGATTTAACTCCAAGAAGTCCTAACTCGGCCATCCTTCTGTCTCCTGTGGAGATC CCATTCAGCAAGGGGGCTATGGAAGCCCATGCAAATCGCCTCATGAGAGAGCTAGATTTTGCAGCCTACA CAGAAGAAAGATTGGACAGTGTCCTCTCACTGTCCCAGGGCAGTGTTGTGAGGCAGTACTGGAGCAGCAA TTTCTTGGTGGATCTACTGGCTGTGGCTGCCCCTGTGGTACCCACTGTTCTGTGGGCATTCAGTACTCAG AGAGGGGGTACAGATCCTGTCTACAACATCGGAGCTTTGCTCCGAGGTTGCTGTGTGGTGGCCCTACACT CACTACGCCGCACCGCTTTCCACATGAAAACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209873 representing BC065045 Red=Cloning site Green=Tags(s) MGPLRESKEQRVQHQEKEISRSRIPRLILRPHRPQQQQQQQNKVSPASESPFSEEESREFNPSSSGRSAR TISSNSFCSDDTGCPSSQSVSPVKTPSDTGHSPIGFCPGSDEDFTRKKCRIGMVGEGSIQSARHKKEPKG GIIKPGSEADFSSSSSTGSISAPEVHMSTTGNKRASFSRNRGPHGRSNGASSHKSGSSPPSPREKDLVSM LCRNPLSPSNIHPSYAPSSPSSSNSGSYKGSDCSPVMRRSGRYMSCGENHGVKPPNPEQYLTPLQQKEVT VRHLRTKLKESERRLHERESEIMELKSQLARMREDWIEEECHRVEAQLALKEARKEIKQLKQVIETMRSS LADKDKGIQKYFVDINIQNKKLESLLQSMEMAHNSSLRDELCLDFSFDSPEKSLPLSSTFDKLPDGLSLE EQITEEGADSELLVGDSMAEGTDLLDEMVTATTTESSGLEFVHSTPGPQALKALPLVSHEEGIAVMEQAV QTDVVPFSPAISELIQSVLKLQDYCPTSSASPDESGADSMESFSESISALMLDLTPRSPNSAILLSPVEI PFSKGAMEAHANRLMRELDFAAYTEERLDSVLSLSQGSVVRQYWSSNFLVDLLAVAAPVVPTVLWAFSTQ RGGTDPVYNIGALLRGCCVVALHSLRRTAFHMKT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC065045
ORF Size	1994 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC065045, AAH65045
RefSeq Size	3139 bp
RefSeq ORF	1994 bp
Locus ID	319613
Gene Summary	Part of a kinesin motor-adapter complex that is critical for the anterograde axonal transport of active zone components and contributes to activity-dependent presynaptic assembly during neuronal development.[UniProtKB/Swiss-Prot Function]

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