Pus7 (BC008544) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	C330017I15Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209845 representing BC008544 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAATGACAAGCACGTCCCTGAAACGTGGGTGCCTTGTTGTGGAAGATAATGACAGTGTGACCCCAC ACGACGAAACAAAGAAACAGAAGGTGTCCGAGGGCTGTCTGACCAGCAGTCAGGATGGGGTAGAGAATGA CGGTCTACACCGCAGTGAGAACGAGCCTGGGCCTCCAGAAGCTGAAAGCACCGTGAAAGATGACGAGAAT TCCAGCGCTCAGGTGCAAGAGGAAGAGGAGGAGGAGGAGGAGGAAGACGGCCTTTCAGAAGCAGGGGAAG AGGAAGAAGCAGAGAGTTTTGCAGACATGATGAAACATGGGCTCACAGAACTTGATGTGGGCATCTGCAA GTTCGTGAGTTCCCATCATGGCTTCTCAGGGATCTTAAAAGAAAGATACTCTGACTTTGTCGTTCACGAA ATCGGGAAAGATGGACGGATCAGCCATTTGGATGACTTATCTGTTCCGGTGGACGAAGAGGACCCTCCAG AAGACGCTCTCACTGTCTTGACAGCTGAGGACAGGCAGCAGTTGGAGGAGCTCCAGCTTTTCAAAAATAA AGAAACCAGTGTTGCCATCGAGGTCATTGAGGACACCAAAGAGAAGCGAACAGTCATCCATCAAGCCATT AAATCTCTCTTCCCAGGACTAGAGACAAAGACAGAGGATAGAGAGGGGCGGAAATACATCGTAGCCTACC ATGCAGCTGGCAAAAAGGCTTTGGCAAATCCAAGAAAACATTCTTGGCCAAAATCCCGGGGAAGTTACTG CCATTTTGTTCTCTACAAGGAAAACAAAGACACCATGGATGCCATCAATGTTCTATCCAAGTACCTGCGG GTAAAGCCAAACATATTCTCCTATATGGGAACCAAAGACAAAAGGGCAATCACAGTCCAGGAGATTGCTG TTCTCAAAATAAGTGCACAAAGACTTGCCCACCTCAATAAGTGCCTGATGAACTTGAAGCTCGGGAATTT CAGCTATCAGAAAACCCCTCTGAAGTTAGGAGCACTCCAAGGAAACCACTTCACTGTTGTTCTCAGGAAT ATAACAGGAACTGATGAGCAGGTGCAGCAAGCCATGCAGTCTCTGAGGGAGACCGGCTTCATTAACTACT ATGGCATGCAAAGGTTCGGGACCACAGCTGTTCCAACGTACCAGGTTGGAAGAGCAATTCTACAGAATTC CTGGACAGAAGTCATGGATTTAATACTGAAACCCCGTTCTGGAGCTGAGAAGGGTTACTTGGTGAAATGC AGAGAAGAATGGGCCAAGACCAAAGACCCAGCCTCTGCTCTCAAAAAGCTGCCCGTCAAAAGGTGTGTGG AAGGGCAGCTGCTCCGAGGACTTTCCAGATATGGAATGAAGAATATAGTCTCTGCATTTGGCATAATTCC AAGAAACAATCGCTTAATGTACATCCACAGTTATCAGAGCTATGTGTGGAACACCATGGTGAGCAGAAGA ATTGAGGAGTACGGGCTGAGGCCTGTGCCCGGGGACCTTGTTCTCAAAGGAGCCACGCCCACCTACATTG AGGAAGATGACGTTGACAACTACTCCATCCACGATGTGGTGATGCCTTTGCCTGGGTTTGATGTTATCTA CCCCAAGCATAAAATCAGTGAGGCCTACAGGGAAATGCTTGCTGCAGACAACCTTGATATTGACAACATG AGACACACGATTCGAGATTACTCCTTATCGGGGGCCTACAGAAGGATCATCATCAGGCCTCAGAGCGTCA GCTGGGAAGTTGTTGCATATGATGATCCTAAAATTCCACTTTTCAACACGGATGTGGACAACCTCGAGGG GAAACCACCACCAGTGTTTGCTTCTGAAGGCAAATACAGGGCTCTGAAGATGGACTTTTCTCTTCCTCCT TCTACTTACGCCACCATGGCCATTCGAGAAGTGCTCAAAATGGACACCAGCATCAAGAACCAGACCCAGC TAAATACGAGCTGGCTGCGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209845 representing BC008544 Red=Cloning site Green=Tags(s) MEMTSTSLKRGCLVVEDNDSVTPHDETKKQKVSEGCLTSSQDGVENDGLHRSENEPGPPEAESTVKDDEN SSAQVQEEEEEEEEEDGLSEAGEEEEAESFADMMKHGLTELDVGICKFVSSHHGFSGILKERYSDFVVHE IGKDGRISHLDDLSVPVDEEDPPEDALTVLTAEDRQQLEELQLFKNKETSVAIEVIEDTKEKRTVIHQAI KSLFPGLETKTEDREGRKYIVAYHAAGKKALANPRKHSWPKSRGSYCHFVLYKENKDTMDAINVLSKYLR VKPNIFSYMGTKDKRAITVQEIAVLKISAQRLAHLNKCLMNLKLGNFSYQKTPLKLGALQGNHFTVVLRN ITGTDEQVQQAMQSLRETGFINYYGMQRFGTTAVPTYQVGRAILQNSWTEVMDLILKPRSGAEKGYLVKC REEWAKTKDPASALKKLPVKRCVEGQLLRGLSRYGMKNIVSAFGIIPRNNRLMYIHSYQSYVWNTMVSRR IEEYGLRPVPGDLVLKGATPTYIEEDDVDNYSIHDVVMPLPGFDVIYPKHKISEAYREMLAADNLDIDNM RHTIRDYSLSGAYRRIIIRPQSVSWEVVAYDDPKIPLFNTDVDNLEGKPPPVFASEGKYRALKMDFSLPP STYATMAIREVLKMDTSIKNQTQLNTSWLR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC008544
ORF Size	1982 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008544, AAH08544
RefSeq Size	3137 bp
RefSeq ORF	1982 bp
Locus ID	78697
Gene Summary	Pseudouridylate synthase that catalyzes pseudouridylation of RNAs. Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types.[UniProtKB/Swiss-Prot Function]

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