Egfr (BC023729) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	9030024J15Rik; AI552599; Erbb; Errb1; Errp; wa-2; wa2; Wa5
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209803 representing BC023729 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGACCCTCAGGGACCGCGAGAACCACACTGCTGGTGTTGCTGACCGCGCTCTGCGCCGCAGGTGGGG CGTTGGAGGAAAAGAAAGTCTGCCAAGGCACAAGTAACAGGCTCACCCAACTGGGCACTTTTGAAGACCA CTTTCTGAGCCTGCAGAGGATGTACAACAACTGTGAAGTGGTCCTTGGGAACTTGGAAATTACCTATGTG CAAAGGAATTACGACCTTTCCTTCTTAAAGACCATCCAGGAGGTGGCCGGCTATGTCCTCATTGCCCTCA ACACCGTGGAGAGAATCCCTTTGGAGAACCTGCAGATCATCAGGGGAAATGCTCTTTATGAAAACACCTA TGCCTTAGCCATCCTGTCCAACTATGGGACAAACAGAACTGGGCTTAGGGAACTGCCCATGCGGAACTTA CAGGAAATCCTGATTGGTGCTGTGCGATTCAGCAACAACCCCATCCTCTGCAATATGGATACTATCCAGT GGAGGGACATCGTCCAAAACGTCTTTATGAGCAACATGTCAATGGACTTACAGAGCCATCCGAGCAGTTG CCCCAAATGTGATCCAAGCTGTCCCAATGGAAGCTGCTGGGGAGGAGGAGAGGAGAACTGCCAGAAATTG ACCAAAATCATCTGTGCCCAGCAATGTTCCCATCGCTGTCGTGGCAGGTCCCCCAGTGACTGCTGCCACA ACCAATGTGCTGCGGGGTGTACAGGGCCCCGAGAGAGTGACTGTCTGGTCTGCCAAAAGTTCCAAGATGA GGCCACATGCAAAGACACCTGCCCACCACTCATGCTGTACAACCCCACCACCTATCAGATGGATGTCAAC CCTGAAGGGAAGTACAGCTTTGGTGCCACCTGTGTGAAGAAGTGCCCCCGAAACTACGTGGTGACAGATC ATGGCTCATGTGTCCGAGCCTGTGGGCCTGACTACTACGAAGTGGAAGAAGATGGCATCCGCAAGTGTAA AAAATGTGATGGGCCCTGTCGCAAAGTTTGTAATGGCATAGGCATTGGTGAATTTAAAGACACACTCTCC ATAAATGCTACAAACATCAAACACTTCAAATACTGCACTGCCATCAGCGGGGACCTTCACATCCTGCCAG TGGCCTTTAAGGGGGATTCTTTCACGCGCACTCCTCCTCTAGACCCACGAGAACTAGAAATTCTAAAAAC CGTAAAGGAAATAACAGGCTTTTTGCTGATTCAGGCTTGGCCTGATAACTGGACTGACCTCCATGCTTTC GAGAACCTAGAAATAATACGTGGCAGAACAAAGCAACATGGTCAGTTTTCTTTGGCGGTCGTTGGCCTGA ACATCACATCACTGGGGCTGCGTTCCCTCAAGGAGATCAGTGATGGGGATGTGATCATTTCTGGAAACCG AAATTTGTGCTACGCAAACACAATAAACTGGAAAAAACTCTTCGGGACACCCAATCAGAAAACCAAAATC ATGAACAACAGAGCTGAGAAAGACTGCAAGGCCGTGAACCACGTCTGCAATCCTTTATGCTCCTCGGAAG GCTGCTGGGGCCCTGAGCCCAGGGACTGTGTCTCCTGCCAGAATGTGAGCAGAGGCAGGGAGTGCGTGGA GAAATGCAACATCCTGGAGGGGGAACCAAGGGAGTTTGTGGAAAATTCTGAATGCATCCAGTGCCATCCA GAATGTCTGCCCCAGGCCATGAACATCACCTGTACAGGCAGGGGACCAGACAACTGCATCCAGTGTGCCC ACTACATTGATGGCCCACACTGTGTCAAGACCTGCCCAGCTGGCATCATGGGAGAGAACAACACTCTGGT CTGGAAGTATGCAGATGCCAATAATGTCTGCCACCTATGCCACGCCAACTGTACCTATGGATGTGCTGGG CCAGGTCTTCAAGGATGTGAAGTGTGGCCATCTGGGTACGTTCAATGGCAGTGGATCTTAAAGACCTTTT GGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209803 representing BC023729 Red=Cloning site Green=Tags(s) MRPSGTARTTLLVLLTALCAAGGALEEKKVCQGTSNRLTQLGTFEDHFLSLQRMYNNCEVVLGNLEITYV QRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNALYENTYALAILSNYGTNRTGLRELPMRNL QEILIGAVRFSNNPILCNMDTIQWRDIVQNVFMSNMSMDLQSHPSSCPKCDPSCPNGSCWGGGEENCQKL TKIICAQQCSHRCRGRSPSDCCHNQCAAGCTGPRESDCLVCQKFQDEATCKDTCPPLMLYNPTTYQMDVN PEGKYSFGATCVKKCPRNYVVTDHGSCVRACGPDYYEVEEDGIRKCKKCDGPCRKVCNGIGIGEFKDTLS INATNIKHFKYCTAISGDLHILPVAFKGDSFTRTPPLDPRELEILKTVKEITGFLLIQAWPDNWTDLHAF ENLEIIRGRTKQHGQFSLAVVGLNITSLGLRSLKEISDGDVIISGNRNLCYANTINWKKLFGTPNQKTKI MNNRAEKDCKAVNHVCNPLCSSEGCWGPEPRDCVSCQNVSRGRECVEKCNILEGEPREFVENSECIQCHP ECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGIMGENNTLVWKYADANNVCHLCHANCTYGCAG PGLQGCEVWPSGYVQWQWILKTFWI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC023729
ORF Size	1965 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC023729, AAH23729
RefSeq Size	2666 bp
RefSeq ORF	1967 bp
Locus ID	13649
Gene Summary	Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:8404850). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (By similarity). Plays a role in enhancing learning and memory performance (PubMed:20639532).[UniProtKB/Swiss-Prot Function]

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