Eml2 (BC055476) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1600029N02Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209755 representing BC055476 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAGTTTTGGAATTGGGAAAACCAAAGAAGTTATCTTCAGCATGGAGGAGGGATCCGTAAAGATGT TTCTGCGGGGACGCCCTGTGCCCATGCTGATCCCGGACGAACTGGCGCCCACCTATAGCCTGGACACTCG TTCTGAGCTGCCCTCCAGCCGGCTAAAGCTAGACTGGGTCTATGGCTATCGAGGCCGGGACTGCAGAGCC AACCTTTACCTCCTGCCCACTGGGGAGGTGGTGTACTTTGTGGCCTCTGTGGCCGTACTGTACAGCGTGG AGGAGCAGAGACAACGACACTACCTGGGGCACAACGATGACATCAAATGTCTGGCTGTCCATCCAGATAT GGTCACCATCGCCACAGGACAGGTGGCAGGCACCACAAAGGAAGGCAAGCCTCTGCCACCCCATGTGCGT GTGTGGGACTCGGTTTCCCTTTCCACCTTACACGTCCTGGGCCTGGGGGTGTTTGACAGAGCTGTGTGCT GTGTGGCCTTCTCCAAATCCAATGGGGGCAACCTGCTATGTGCAGTGGACGAATCCAACGACCATGTGCT CTCTGTGTGGGACTGGGCCAAGGAGAGCAAAGTGGTGGATAGCAAGTGCTCTAACGAGGCAGTGCTAGTG GCCACCTTCCACCCCACTGATCCCAGCCTGCTGATAACCTGTGGCAAATCCCACATCTACTTCTGGAGCC TGGAGGGAGGCAGTCTGAGCAAGAGGCAGGGCCTGTTCGAGAAACACGAGAAACCAAAGTACGTGCTGTG TGTGACCTTCCTGGAAGGTGGCGATGTGGTCACTGGAGACTCTGGGGGGAACCTCTATGTCTGGGGCAAA GGTGGAAACCGCATCACCCAGGAAGTTCAAGGAGCCCATGATGGTGGCGTGTTTGCTCTCTGCGCCCTGC GGGACGGGACGCTGGTGTCTGGAGGGGGCCGCGATCGTAGGGTGGTCCTCTGGGGTTCGGACTACAGCAA GGTGCAGGAGGTGGAGGTCCCAGAGGACTTCGGCCCTGTACGCACCGTGGCAGAGGGCCGGGGAGACACA CTGTACGTGGGGACCACCCGTAACTCCATCTTGCTAGGCTCGGTGCACACAGGGTTTTCGCTGCTTGTCC AGGGACATGTGGAGGAGCTGTGGGGCCTGGCCACACATCCTAGCAGGGCACAGTTTGTGACCTGTGGGCA GGACAAGCTGGTGCATCTGTGGAGTTCCGAGACCCACCAGCCAGTGTGGAGCAGGAGCATCGAGGACCCT GCCCGTTCGGCTGGCTTCCACCCCAGTGGCTCCGTCCTAGCGGTGGGCACAGTGACCGGCAGGTGGCTGC TACTGGACACAGAGACCCATGACCTGGTGGCTATCCACACAGATGGCAATGAGCAGATCTCCGTGGTCAG CTTTTCACCAGACGGGGCGTACCTGGCTGTGGGCTCCCACGACAACTTGGTGTACGTGTACACGGTGGAC CAGGGTGGCCGCAAGGTCAGCCGCCTGGGCAAGTGCTCGGGCCATTCCAGTTTTATCACCCACCTGGATT GGGCCCAGGACAGCACCTGCTTTGTCACCAACTCTGGAGACTACGAGATTCTGTACTGGGACCCCGTCAC CTGTAAGCAAATCACCAGTGCAGACACCGTGAGAAACGTGGAATGGGCTACCGCCACTTGTGTGTTGGGT TTTGGAGTGTTTGGGATTTGGCCCGAGGGAGCAGATGGCACAGACATCAATGCTGTGGCCCGCTCTCATG ATGGGAAGTTGCTGGTTTCTGCTGACGACTTTGGAAAAGTGCACCTGTTCAGCTACCCCTGCTGTCAGCC GAGAGCCCTCAGCCACAAATACGGTGGACACAGCAGCCATGTGACTAATGTGGCCTTCTTGTGGGATGAC AGCATGGCCCTAACCACTGGGGGCAAAGACACGAGCGTGCTGCAGTGGCGGGTGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209755 representing BC055476 Red=Cloning site Green=Tags(s) MSSFGIGKTKEVIFSMEEGSVKMFLRGRPVPMLIPDELAPTYSLDTRSELPSSRLKLDWVYGYRGRDCRA NLYLLPTGEVVYFVASVAVLYSVEEQRQRHYLGHNDDIKCLAVHPDMVTIATGQVAGTTKEGKPLPPHVR VWDSVSLSTLHVLGLGVFDRAVCCVAFSKSNGGNLLCAVDESNDHVLSVWDWAKESKVVDSKCSNEAVLV ATFHPTDPSLLITCGKSHIYFWSLEGGSLSKRQGLFEKHEKPKYVLCVTFLEGGDVVTGDSGGNLYVWGK GGNRITQEVQGAHDGGVFALCALRDGTLVSGGGRDRRVVLWGSDYSKVQEVEVPEDFGPVRTVAEGRGDT LYVGTTRNSILLGSVHTGFSLLVQGHVEELWGLATHPSRAQFVTCGQDKLVHLWSSETHQPVWSRSIEDP ARSAGFHPSGSVLAVGTVTGRWLLLDTETHDLVAIHTDGNEQISVVSFSPDGAYLAVGSHDNLVYVYTVD QGGRKVSRLGKCSGHSSFITHLDWAQDSTCFVTNSGDYEILYWDPVTCKQITSADTVRNVEWATATCVLG FGVFGIWPEGADGTDINAVARSHDGKLLVSADDFGKVHLFSYPCCQPRALSHKYGGHSSHVTNVAFLWDD SMALTTGGKDTSVLQWRVA TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC055476
ORF Size	1949 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC055476, AAH55476
RefSeq Size	2347 bp
RefSeq ORF	1949 bp
Locus ID	72205
Gene Summary	Tubulin binding protein that inhibits microtubule nucleation and growth, resulting in shorter microtubules.[UniProtKB/Swiss-Prot Function]

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