Pip5k1c (BC019138) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI115456; AI835305; Pip5kIgamma
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209646 representing BC019138 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTAGAGGTGCCGGACGAGGCGGAGAGCGCCGAAGCGGGGGCCGTGACGGCGGAAGCGGCCTGGT CTGCGGAGAGTGGGGCGGCGGCAGGTATGACCCAGAAGAAGGCTGGCCTTGCAGAGGCCCCCTTGGTGAC AGGGCAGCCAGGCCCTGGCCATGGGAAGAAGCTGGGCCACCGAGGCGTGGATGCGTCGGGAGAGACTACG TATAAGAAGACCACCTCGTCCACCCTGAAGGGCGCCATCCAGCTGGGGATCGGGTACACGGTGGGCAACC TGAGCTCTAAGCCGGAGCGGGACGTGCTCATGCAGGACTTCTACGTGGTGGAGAGCATCTTCTTCCCCAG TGAAGGGAGCAACCTCACCCCTGCCCACCACTTCCAGGATTTCCGCTTCAAGACCTATGCACCTGTTGCC TTCCGCTACTTCCGGGAGCTCTTTGGCATCCGTCCAGATGACTATTTGTACTCCCTGTGCAATGAGCCAC TCATCGAGCTCTCCAACCCCGGGGCCAGCGGCTCTGTCTTCTACGTCACCAGCGACGACGAGTTCATCAT CAAGACTGTCATGCACAAGGAGGCAGAGTTCCTGCAGAAGCTGCTGCCTGGCTACTACATGAATCTCAAC CAGAACCCACGGACGCTGCTGCCCAAGTTCTATGGGCTGTACTGCGTGCAGTCTGGTGGCAAGAACATCC GCGTGGTGGTCATGAACAATGTGCTGCCCCGTGTCGTCAAAATGCACCTTAAGTTCGACCTCAAGGGCTC CACGTACAAGCGCAGGGCCAGCAAGAAGGAGAAGGAGAAGAGCCTGCCCACCTACAAGGACCTGGACTTC ATGCAGGACATGCCCGAGGGGCTGCTGCTGGACTCCGACACCTTTGGCGCCCTGGTCAAGACGCTGCAGC GAGACTGCCTGGTGCTGGAGAGCTTCAAGATAATGGACTACAGCCTGCTGCTGGGCGTGCACAACATCGA TCAGCAGGAGCGAGAGCGCCAGGCCGAGGGCGCCCAGAGCAAGGCGGATGAGAAGCGGCCCGTGGCCCAG AAGGCTCTGTATTCCACGGCCATGGAGTCTATCCAGGGCGGAGCTGCCCGTGGGGAGGCCATTGAGACAG ATGACACGATGGGTGGGATTCCAGCAGTGAATGGGCGCGGGGAGCGACTGCTCCTCCACATCGGGATCAT TGATATTCTGCAGTCCTACAGGTTCATCAAGAAGTTAGAACACACCTGGAAGGCCCTCGTCCATGATGGG GACACTGTCTCAGTCCACCGGCCCAGCTTCTATGCAGAGCGCTTCTTCAAGTTCATGAGCAGCACGGTGT TCCGGAAGAGTTCCTCCCTGAAGTCCTCTCCATCCAAGAAAGGACGTGGTGCCCTGCTGGCGGTCAAACC CCTGGGGCCCACTGCTGCCTTCTCAGCCAGCCAGATCCCCAGCGAGAGAGAGGATGTGCAGTATGACCTG CGGGGGGCCCGCAGCTACCCCACGCTTGAGGATGAAGGCCGGCCTGACCTCCTGCCCTGCACCCCACCGT CCTTTGAGGAAGCCACCACAGCCTCCATCGCCACCACCCTGTCGTCCACCTCCCTCTCCATCCCAGAGCG GTCCCCTTCAGATACATCGGAGCAGCCCCGGTACAGGCGGCGCACGCAGTCTTCAGGCCAGGATGGCCGG CCCCAGGAGGAGCCCCATGCGGAAGACCTGCAGAAGATAACTGTGCAGGTGGAGCCAGTGTGCGGTGTGG GGGTTGTCCCCAAGGAGGAGGGTGCAGGAGTGGAGGTCCCCCCATGTGGGGCATCGGCTGCAGCCTCTGT GGAAATAGACGCTGCCAGCCAGGCCTCAGAGCCTGCCAGCCAGGCCTCAGATGAGGAGGATGCACCCTCT ACAGACATCTATTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209646 representing BC019138 Red=Cloning site Green=Tags(s) MELEVPDEAESAEAGAVTAEAAWSAESGAAAGMTQKKAGLAEAPLVTGQPGPGHGKKLGHRGVDASGETT YKKTTSSTLKGAIQLGIGYTVGNLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFQDFRFKTYAPVA FRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSVFYVTSDDEFIIKTVMHKEAEFLQKLLPGYYMNLN QNPRTLLPKFYGLYCVQSGGKNIRVVVMNNVLPRVVKMHLKFDLKGSTYKRRASKKEKEKSLPTYKDLDF MQDMPEGLLLDSDTFGALVKTLQRDCLVLESFKIMDYSLLLGVHNIDQQERERQAEGAQSKADEKRPVAQ KALYSTAMESIQGGAARGEAIETDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVHDG DTVSVHRPSFYAERFFKFMSSTVFRKSSSLKSSPSKKGRGALLAVKPLGPTAAFSASQIPSEREDVQYDL RGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSDTSEQPRYRRRTQSSGQDGR PQEEPHAEDLQKITVQVEPVCGVGVVPKEEGAGVEVPPCGASAAASVEIDAASQASEPASQASDEEDAPS TDIYF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC019138
ORF Size	1907 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC019138, AAH19138
RefSeq Size	4226 bp
RefSeq ORF	1907 bp
Locus ID	18717
Gene Summary	Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as vesicle mediated transport, cell adhesion, cell polarization and cell migration. Together with PIP5K1A is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport. Controls the plasma membrane pool of PtdIns(4,5)P2 implicated in synaptic vesicle endocytosis and exocytosis. Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2). Required for clathrin-coated pits assembly at the synapse. Participates in cell junction assembly. Modulates adherens junctions formation by facilitating CDH1 trafficking. Required for focal adhesion dynamics. Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions. Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins. Required for uropodium formation and retraction of the cell rear during directed migration. Has a role in growth factor- stimulated directional cell migration and adhesion. Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor. Negative regulator of T-cell activation and adhesion. Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor. Together with PIP5K1A has a role during embryogenesis and together with PIP5K1B may have a role immediately after birth.[UniProtKB/Swiss-Prot Function]

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