Pip5k1c (BC019138) Mouse Tagged ORF Clone

CAT#: MG209646

  • TrueORF®

Pip5k1c (tGFP-tagged) - Mouse phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma (cDNA clone MGC:29231 IMAGE:5041431)



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Mouse Protein / Lysate Request

CNY 5,040.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AI115456; AI835305; Pip5kIgamma
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209646 representing BC019138
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTAGAGGTGCCGGACGAGGCGGAGAGCGCCGAAGCGGGGGCCGTGACGGCGGAAGCGGCCTGGT
CTGCGGAGAGTGGGGCGGCGGCAGGTATGACCCAGAAGAAGGCTGGCCTTGCAGAGGCCCCCTTGGTGAC
AGGGCAGCCAGGCCCTGGCCATGGGAAGAAGCTGGGCCACCGAGGCGTGGATGCGTCGGGAGAGACTACG
TATAAGAAGACCACCTCGTCCACCCTGAAGGGCGCCATCCAGCTGGGGATCGGGTACACGGTGGGCAACC
TGAGCTCTAAGCCGGAGCGGGACGTGCTCATGCAGGACTTCTACGTGGTGGAGAGCATCTTCTTCCCCAG
TGAAGGGAGCAACCTCACCCCTGCCCACCACTTCCAGGATTTCCGCTTCAAGACCTATGCACCTGTTGCC
TTCCGCTACTTCCGGGAGCTCTTTGGCATCCGTCCAGATGACTATTTGTACTCCCTGTGCAATGAGCCAC
TCATCGAGCTCTCCAACCCCGGGGCCAGCGGCTCTGTCTTCTACGTCACCAGCGACGACGAGTTCATCAT
CAAGACTGTCATGCACAAGGAGGCAGAGTTCCTGCAGAAGCTGCTGCCTGGCTACTACATGAATCTCAAC
CAGAACCCACGGACGCTGCTGCCCAAGTTCTATGGGCTGTACTGCGTGCAGTCTGGTGGCAAGAACATCC
GCGTGGTGGTCATGAACAATGTGCTGCCCCGTGTCGTCAAAATGCACCTTAAGTTCGACCTCAAGGGCTC
CACGTACAAGCGCAGGGCCAGCAAGAAGGAGAAGGAGAAGAGCCTGCCCACCTACAAGGACCTGGACTTC
ATGCAGGACATGCCCGAGGGGCTGCTGCTGGACTCCGACACCTTTGGCGCCCTGGTCAAGACGCTGCAGC
GAGACTGCCTGGTGCTGGAGAGCTTCAAGATAATGGACTACAGCCTGCTGCTGGGCGTGCACAACATCGA
TCAGCAGGAGCGAGAGCGCCAGGCCGAGGGCGCCCAGAGCAAGGCGGATGAGAAGCGGCCCGTGGCCCAG
AAGGCTCTGTATTCCACGGCCATGGAGTCTATCCAGGGCGGAGCTGCCCGTGGGGAGGCCATTGAGACAG
ATGACACGATGGGTGGGATTCCAGCAGTGAATGGGCGCGGGGAGCGACTGCTCCTCCACATCGGGATCAT
TGATATTCTGCAGTCCTACAGGTTCATCAAGAAGTTAGAACACACCTGGAAGGCCCTCGTCCATGATGGG
GACACTGTCTCAGTCCACCGGCCCAGCTTCTATGCAGAGCGCTTCTTCAAGTTCATGAGCAGCACGGTGT
TCCGGAAGAGTTCCTCCCTGAAGTCCTCTCCATCCAAGAAAGGACGTGGTGCCCTGCTGGCGGTCAAACC
CCTGGGGCCCACTGCTGCCTTCTCAGCCAGCCAGATCCCCAGCGAGAGAGAGGATGTGCAGTATGACCTG
CGGGGGGCCCGCAGCTACCCCACGCTTGAGGATGAAGGCCGGCCTGACCTCCTGCCCTGCACCCCACCGT
CCTTTGAGGAAGCCACCACAGCCTCCATCGCCACCACCCTGTCGTCCACCTCCCTCTCCATCCCAGAGCG
GTCCCCTTCAGATACATCGGAGCAGCCCCGGTACAGGCGGCGCACGCAGTCTTCAGGCCAGGATGGCCGG
CCCCAGGAGGAGCCCCATGCGGAAGACCTGCAGAAGATAACTGTGCAGGTGGAGCCAGTGTGCGGTGTGG
GGGTTGTCCCCAAGGAGGAGGGTGCAGGAGTGGAGGTCCCCCCATGTGGGGCATCGGCTGCAGCCTCTGT
GGAAATAGACGCTGCCAGCCAGGCCTCAGAGCCTGCCAGCCAGGCCTCAGATGAGGAGGATGCACCCTCT
ACAGACATCTATTTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209646 representing BC019138
Red=Cloning site Green=Tags(s)

MELEVPDEAESAEAGAVTAEAAWSAESGAAAGMTQKKAGLAEAPLVTGQPGPGHGKKLGHRGVDASGETT
YKKTTSSTLKGAIQLGIGYTVGNLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFQDFRFKTYAPVA
FRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSVFYVTSDDEFIIKTVMHKEAEFLQKLLPGYYMNLN
QNPRTLLPKFYGLYCVQSGGKNIRVVVMNNVLPRVVKMHLKFDLKGSTYKRRASKKEKEKSLPTYKDLDF
MQDMPEGLLLDSDTFGALVKTLQRDCLVLESFKIMDYSLLLGVHNIDQQERERQAEGAQSKADEKRPVAQ
KALYSTAMESIQGGAARGEAIETDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVHDG
DTVSVHRPSFYAERFFKFMSSTVFRKSSSLKSSPSKKGRGALLAVKPLGPTAAFSASQIPSEREDVQYDL
RGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSDTSEQPRYRRRTQSSGQDGR
PQEEPHAEDLQKITVQVEPVCGVGVVPKEEGAGVEVPPCGASAAASVEIDAASQASEPASQASDEEDAPS
TDIYF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC019138
ORF Size 1907 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC019138, AAH19138
RefSeq Size 4226 bp
RefSeq ORF 1907 bp
Locus ID 18717
Gene Summary Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as vesicle mediated transport, cell adhesion, cell polarization and cell migration. Together with PIP5K1A is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport. Controls the plasma membrane pool of PtdIns(4,5)P2 implicated in synaptic vesicle endocytosis and exocytosis. Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2). Required for clathrin-coated pits assembly at the synapse. Participates in cell junction assembly. Modulates adherens junctions formation by facilitating CDH1 trafficking. Required for focal adhesion dynamics. Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions. Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins. Required for uropodium formation and retraction of the cell rear during directed migration. Has a role in growth factor- stimulated directional cell migration and adhesion. Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor. Negative regulator of T-cell activation and adhesion. Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor. Together with PIP5K1A has a role during embryogenesis and together with PIP5K1B may have a role immediately after birth.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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