Jph4 (BC052722) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	JP-4, JPHL1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209574 representing BC052722 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCCCGGGGGCAAGTTCGACTTTGACGACGGGGGCTGCTACGTGGGGGGCTGGGAGGCGGGGCGGG CACATGGCTACGGCGTGTGCACCGGGCCCGGCGCCCAGGGAGAGTACAGCGGCTGCTGGGCTCACGGCTT CGAGTCGCTGGGTGTCTTCACGGGGCCCGGCGGACACAGTTACCAGGGCCACTGGCAGCAGGGCAAGCGC GAAGGGCTGGGCGTGGAGCGCAAGAGCCGCTGGACCTACCGCGGCGAGTGGCTGGGCGGGCTGAAGGGGC GCAGCGGCGTATGGGAGAGCGTGTCCGGCCTGCGCTACGCCGGGCTCTGGAAGGACGGCTTCCAGGACGG CTACGGCACCGAGACCTACTCGGATGGAGGCACTTACCAAGGCCAGTGGCAAGCCGGGAAACGCCACGGC TACGGGGTGCGCCAAAGTGTACCCTACCACCAGGCGGCGCTTCTGCGCTCACCCCGCCGCACCTCCCTGG ACTCCGGCCACAGCGACCCCCCGACGCCGCCTCCACCCCTACCCCTACCAGGAGATGAAGGAGGCAGCCC AGCCTCCGGCTCCCGAGGCGGCTTCGTGCTGGCGGGGCCAGGGGACGCCGACGGGGCGTCTTCCCGTAAG CGCACCCCAGCAGCAGGTGGATTCTTCCGCCGTTCACTGCTGCTCAGCGGGCTCCGGGCTGGTGGGCGCC GCAGCTCCCTGGGCAGCAAGAGGGGATCTCTACGCAGCGAGGTGAGCAGCGAAGTGGGCAGTACAGGACC CCCGGGCTCCGAGGCCAGCGGGCCCCCAATCCCAGCTCCACCTGCCCTCATCGAGGGCTCAGCCACTGAG GTGTACGCCGGTGAGTGGCGTGCGGACCGGCGCAGTGGCTATGGAGTGAGCCAGAGATCCAACGGGCTGC GCTACGAAGGCGAGTGGCTGGGCAACCGACGCCACGGATACGGGCGCACCACCCGGCCCGATGGCTCCCG TGAGGAGGGCAAGTACAAGCGTAACCGGCTGGTACACGGGGGACGTGTCCGCAGCCTCCTGCCTCTGGCC CTTAGACGGGGCAAAGTCAAGGAGAAAGTGGACAGGGCTGTCGAAGGTGCCCGTCGAGCTGTGAGTGCTG CCCGCCAGCGCCAGGAAATTGCGGCTGCCAGGGCAGCAGACGCCCTCCTAAAAGCAGTGGCAGCCAGCAG CGTCGCGGAGAAGGCTGTGGAGGCTGCGCGGATGGCCAAGCTGATAGCCCAGGATCTACAACCCATGTTA GAGGCCCCAGGCCGCAGACCCAGACAGGACTCAGGAGGTTCTGACACAGAGCCTTTGGATGAGGACAGCC CTGGGGTATACGAGAATGGACTGACCCCCTCAGAGGGCTCTCCGGAACTACCCAGCAGCCCCGCCTCCTC CCACCAACCTTGGCGAGCCCCTGCCTGCCGGAGTCCACTGCCTCCTGGAGGGAACTGGGGCCCCTTCTCC AGCCCTAAAGCTTGGCCCGAGGAGTGGGGGGGTCCTGGTGAGCAGGCAGAGGAACTAGCTGGCTATGAGG CTGAGGATGAGGCTGGGGTGCAGGGTCCAGGACCAAGAGATGGTTCCCCACTTCTCGGAGGCTGCAGTGA CAGTTCAGGAAGTCTTCGAGAGGAGGAGGGGGAAGACGAAGAGTCCTTGCCCCAACTGAGGGCCCCAGGA GGCTCAGAGTCTGAGCCTGTCACCACGCCAGTTTTGAGGGGCCTGTCTTCAAGGGGTCCTGATGCTGGGT GCCTGACAGAAGAGGTTGAGGAGCCTGCTCCCACCGAGAGGCCTGCCCAGCCGGGAGCTGCCAACCCCCT GGTGGTGGGAGCCGTGGCCTTGCTGGACCTCAGCCTGGCCTTCCTGTTCTCCCAGCTCCTCACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209574 representing BC052722 Red=Cloning site Green=Tags(s) MSPGGKFDFDDGGCYVGGWEAGRAHGYGVCTGPGAQGEYSGCWAHGFESLGVFTGPGGHSYQGHWQQGKR EGLGVERKSRWTYRGEWLGGLKGRSGVWESVSGLRYAGLWKDGFQDGYGTETYSDGGTYQGQWQAGKRHG YGVRQSVPYHQAALLRSPRRTSLDSGHSDPPTPPPPLPLPGDEGGSPASGSRGGFVLAGPGDADGASSRK RTPAAGGFFRRSLLLSGLRAGGRRSSLGSKRGSLRSEVSSEVGSTGPPGSEASGPPIPAPPALIEGSATE VYAGEWRADRRSGYGVSQRSNGLRYEGEWLGNRRHGYGRTTRPDGSREEGKYKRNRLVHGGRVRSLLPLA LRRGKVKEKVDRAVEGARRAVSAARQRQEIAAARAADALLKAVAASSVAEKAVEAARMAKLIAQDLQPML EAPGRRPRQDSGGSDTEPLDEDSPGVYENGLTPSEGSPELPSSPASSHQPWRAPACRSPLPPGGNWGPFS SPKAWPEEWGGPGEQAEELAGYEAEDEAGVQGPGPRDGSPLLGGCSDSSGSLREEEGEDEESLPQLRAPG GSESEPVTTPVLRGLSSRGPDAGCLTEEVEEPAPTERPAQPGAANPLVVGAVALLDLSLAFLFSQLLT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC052722
ORF Size	1884 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC052722, AAH52722
RefSeq Size	3571 bp
RefSeq ORF	1886 bp
Locus ID	319984
Gene Summary	Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH4 is brain-specific and appears to have an active role in certain neurons involved in motor coordination and memory.[UniProtKB/Swiss-Prot Function]

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