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Evc (BC021464) Mouse Tagged ORF Clone
CAT＃: MG209571
- TrueORF®
Evc (tGFP-tagged) - Mouse Ellis van Creveld gene homolog (human) (cDNA clone MGC:29404 IMAGE:5069004)
ORF Plasmid: DDK
"BC021464" in other vectors (4)
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CNY 6,940.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209571 representing BC021464
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCTGCACTAAAGATGCGCGGCTGCAGCTGGGCCGGGAAGCCCTGCAGGCGGCGCCGACGCTGCTGG TCCCCGCGGTGCTACTGGGTGGCGTTCTCGGCCTAGGCCTTGGCTTGTGGCTTGGCTGTCGCGCGAGCCA CTTGCGCGCACGGCTCCAGAAAGATGACAGGAAACGTCTGCTCGGGAGTTCAGAGCCCCCTGCACAGAGC CTACGGGACACTGGCTCTCAAGCCAGGAGGCGCCAGAGAGAAACCGCTCGGGATGAAGACGCCCCTGAGG TGTGTGAGCCATCCTTGAGTGGCAACATCACGGCATTTGCACTGAAAGCCAGAGTTGTCTATCCCATCAA CCAGAAGTTCCGGCCTCTGGCCGACGGATCCTCCCACCCATCGCTGCATGAAAACCTGACACAGGCAGCG GCCATCCTGCCCCACCTGCCCCACCAGCCAGCAGAGGCCTCACCAGCCAGCAGCCTGGGCAGCCTGAGCC AGGCTGGCAAGGAGGACGGCAGCTCCTCCTCCAGCATGCGGTCAACCTACAGCGACGACCGGATCCTCCA GTGTGCCTTTCTCCGGGTGGGCAGCTTCCCTGAGATCCTGGCCTGTGAGAGTGTGGACATTGACCTGTGT GTCTGCAGCCTTCACCTGAAAGACCTGCTTCAGGTAGACACGGCGCTGAGGCAAGAGAAGCACCTGATGT TCATTCAGATTCTTAAAGCATGCCTCCTGGATTTTTTTCCTAAAAAGAAGCCTGATGATGAATTATGCCA GAAGGTTCTTTCCAAACAAGAGCATGATTTGGAGGAATTAGAAAAGGGACTCCAGGCCAGATTGGCGAAC ACAGAAATGTTAGGCACTGGCGACTCTGGCTACGTTTCCCTGGCAGATGTGGAGAGGAAGGAGAGAGAGC TCTCAGAGCAGCTCATAGACAACATGGGAGCTTTCTGGAAGCAGATGGAAAGCATCCAGCCCACTCTCAT GGACCAGTTTAAGTGTTCCAGCTCCAAAGCTCGGCAGTTCATGATGACTCTGACAGGAAGAATGATTGTC GCTGAAGGACTGCTGCATGACTCTCAGGACCTGCATGTCCTGGACACCCTGGAGAGGACAATGGGTCGAG CCCACTTGGCAAGAATGGTGGAGTTCCTGAGAACCCAGATCCAGGAGGAGACCAAGTGCAGACTTGCTGC CATCTCCCGTGGCCTGGAGTTGCTGACTGTCCAGGGGCAGCTGTCTGGGAGGCAGAAGGAGGAACTCCTG ACTCAGCAGCACAAGGCCTTCTGGGAGGAGGCAGAGCGTTTTGGCAGGGAATTCATGCAGCGGGGCAAAG ATCTGGTCCAGGCGTCACAGGCTCGCCAGGCAGAGGCAGCAGCAGAGCTCACACAGACCCAAGAAGAAGA ACGGAGGAGTTTCCTGGCTGATTCCCAGCTGACCTCGGATCCTGGGGAATTCCTCAAGGCTTTTCATGAG GTCCTGGAGAGGCAGCGGCTGACAAGGAGTGACCAGGAGGGAGACGAGGACACCTGGATCACTGAGGCCA TGGCTGCGCTCTGCCAGGAGCTGTACTGTAGCACCATGGGAACCTTCCAAAAGTTTGTGGACTCCCTGTT CCTGAAGACCCTCCCAGAAGTGACCAGCCTCCCTGTGGGAGAATGTGAGACCCTGCGGCAGCAGGTCCAG GAGCAGGCAGCAAGGCAGCTGGGACAGGCAGACCGATTCCGCAGACGACAGTGGGGACTCCTATGTGATC TATTGGAGCAGGACAAACGGGTGTGGCTGGAGGAGGGTACGCTGTCCACGGTGCTGCAGAGGCAGCTGCG GGATCACCACGAGAGCACCATCCACGGCGTCCTGAGCAGATTCAGTGGCCTTTCTGAAGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209571 representing BC021464
Red=Cloning site Green=Tags(s) MTCTKDARLQLGREALQAAPTLLVPAVLLGGVLGLGLGLWLGCRASHLRARLQKDDRKRLLGSSEPPAQS LRDTGSQARRRQRETARDEDAPEVCEPSLSGNITAFALKARVVYPINQKFRPLADGSSHPSLHENLTQAA AILPHLPHQPAEASPASSLGSLSQAGKEDGSSSSSMRSTYSDDRILQCAFLRVGSFPEILACESVDIDLC VCSLHLKDLLQVDTALRQEKHLMFIQILKACLLDFFPKKKPDDELCQKVLSKQEHDLEELEKGLQARLAN TEMLGTGDSGYVSLADVERKERELSEQLIDNMGAFWKQMESIQPTLMDQFKCSSSKARQFMMTLTGRMIV AEGLLHDSQDLHVLDTLERTMGRAHLARMVEFLRTQIQEETKCRLAAISRGLELLTVQGQLSGRQKEELL TQQHKAFWEEAERFGREFMQRGKDLVQASQARQAEAAAELTQTQEEERRSFLADSQLTSDPGEFLKAFHE VLERQRLTRSDQEGDEDTWITEAMAALCQELYCSTMGTFQKFVDSLFLKTLPEVTSLPVGECETLRQQVQ EQAARQLGQADRFRRRQWGLLCDLLEQDKRVWLEEGTLSTVLQRQLRDHHESTIHGVLSRFSGLSEE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC021464 |
| ORF Size | 1881 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC021464.1 |
| RefSeq Size | 2209 bp |
| RefSeq ORF | 1883 bp |
| Locus ID | 59056 |
| Gene Summary | Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling (PubMed:17660199, PubMed:24582806). Involved in endochondral growth and skeletal development (PubMed:17660199).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218350 | Evc (untagged) - Mouse Ellis van Creveld gene homolog (human) (cDNA clone MGC:29404 IMAGE:5069004), (10ug) |
CNY 6,940.00 |
|
| MR209571 | Evc (Myc-DDK-tagged) - Mouse Ellis van Creveld gene homolog (human) (cDNA clone MGC:29404 IMAGE:5069004) |
CNY 6,270.00 |
|
| MR209571L3 | Lenti ORF clone of Evc (Myc-DDK-tagged) - Mouse Ellis van Creveld gene homolog (human) (cDNA clone MGC:29404 IMAGE:5069004) |
CNY 8,170.00 |
|
| MR209571L4 | Lenti ORF clone of Evc (mGFP-tagged) - Mouse Ellis van Creveld gene homolog (human) (cDNA clone MGC:29404 IMAGE:5069004) |
CNY 8,170.00 |
