Cul4b (BC004026) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2700050M05Rik; AA409770; CUL-4B; mKIAA0695
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209424 representing BC004026 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAATGATCAGGAGCATTTTTTTGTTTCTGGATAGAACTTATGTTCTTCAGAATTCAATGCTACCCT CCATTTGGGACATGGGATTAGAGTTATTTAGGGCTCATATTATAAGTGATCAAAAAGTACAGACAAAAAC GATTGATGGCATCCTTCTCTTGATTGAGAGAGAGCGGAATGGTGAAGCAATTGATAGAAGTTTACTTCGA AGCCTTTTAAGTATGCTGTCTGATTTGCAAATTTACCAAGATTCTTTTGAACAGCAATTTTTGCAAGAAA CTAATCGGCTTTACGCAGCCGAAGGTCAGAAGTTAATGCAAGAAAGAGAGGTTCCTGAGTATCTTCATCA TGTTAATAAACGCCTAGAAGAAGAAGCTGACAGACTTATTACTTACTTAGATCAGACCACCCAGAAGTCA CTAATTGCTTCTGTTGAAAAACAACTTCTAGGTGAACACTTAACGGCAATTCTTCAAAAAGGTTTAAATA GCCTCCTTGATGAAAACCGAATTCAAGATTTGTCTCTCCTGTATCAGCTCTTCAGTAGAGTTCGGGGTGG AGTTCAGGTTCTCTTACAGCAGTGGATTGAGTATATCAAGGCTTTTGGAAGTACCATTGTTATTAATCCT GAAAAAGATAAAACTATGGTTCAAGAATTATTGGACTTTAAAGATAAAGTTGACCATATAATTGATACCT GCTTTCTGAAAAATGAAAAATTTATCAATGCCATGAAAGAAGCTTTTGAAACATTCATTAATAAAAGACC AAATAAGCCAGCTGAACTGATAGCTAAGTATGTGGATTCAAAACTTCGTGCAGGCAACAAAGAAGCTACA GATGAAGAGCTTGAGAAAATGTTGGATAAAATAATGATTATATTTAGATTTATCTATGGTAAAGATGTTT TTGAGGCCTTCTATAAGAAAGATTTAGCCAAACGCCTATTAGTTGGCAAGAGTGCATCAGTAGATGCTGA AAAATCAATGCTGTCCAAACTGAAACACGAATGTGGAGCCGCTTTTACCAGCAAGCTTGAAGGAATGTTC AAAGACATGGAGCTTTCTAAAGATATCATGATTCAGTTTAAACAGTATATGCAGAACCAGAATGTACCTG GCAATATTGAATTAACTGTTAATATCCTGACAATGGGTTACTGGCCAACATATGTGCCTATGGAAGTCCA TTTGCCGCCAGAGATGGTAAAGCTTCAGGAAATTTTCAAGACATTTTACTTAGGCAAGCACAGTGGCAGG AAACTCCAGTGGCAGTCAACCTTGGGACACTGTGTGCTAAAAGCTGAATTTAAAGAGGGCAAAAAAGAAC TTCAGGTCTCTCTTTTTCAAACCATGGTGCTGCTAATGTTTAATGAAGGAGAAGAATTCAGTTTAGAAGA GATCAAGCATGCTACTGGGATAGAGGATGGAGAATTAAGAAGAACACTGCAGTCACTAGCCTGTGGCAAA GCTAGAGTTCTGGCTAAAAATCCTAAGGGCAAAGATATTGAAGATGGTGACAAATTTATTTGTAATGACG ATTTCAAACACAAACTATTCAGGATAAAGATCAATCAAATCCAGATGAAAGAAACGGTTGAAGAACAAGC AAGCACTACAGAAAGAGTATTTCAAGACAGACAGTACCAAATTGATGCTGCGATCGTTCGAATTATGAAG ATGAGAAAAACACTTAGCCACAATCTTCTTGTTTCAGAAGTCTACAATCAGCTAAAATTTCCAGTTAAAC CTGCTGATCTTAAGAAGAGAATAGAATCCCTCATTGACCGGGACTACATGGAAAGAGATAAAGAAAATCC AAATCAGTACAACTATATTGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209424 representing BC004026 Red=Cloning site Green=Tags(s) MIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQTKTIDGILLLIERERNGEAIDRSLLR SLLSMLSDLQIYQDSFEQQFLQETNRLYAAEGQKLMQEREVPEYLHHVNKRLEEEADRLITYLDQTTQKS LIASVEKQLLGEHLTAILQKGLNSLLDENRIQDLSLLYQLFSRVRGGVQVLLQQWIEYIKAFGSTIVINP EKDKTMVQELLDFKDKVDHIIDTCFLKNEKFINAMKEAFETFINKRPNKPAELIAKYVDSKLRAGNKEAT DEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMF KDMELSKDIMIQFKQYMQNQNVPGNIELTVNILTMGYWPTYVPMEVHLPPEMVKLQEIFKTFYLGKHSGR KLQWQSTLGHCVLKAEFKEGKKELQVSLFQTMVLLMFNEGEEFSLEEIKHATGIEDGELRRTLQSLACGK ARVLAKNPKGKDIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQASTTERVFQDRQYQIDAAIVRIMK MRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYNYIA TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC004026
ORF Size	1844 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC004026, AAH04026
RefSeq Size	3105 bp
RefSeq ORF	1844 bp
Locus ID	72584
Gene Summary	Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8 (By similarity). With CUL4A, contributes to ribosome biogenesis (By similarity).[UniProtKB/Swiss-Prot Function]

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