Tec (BC037071) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209373 representing BC037071 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTTCAACACTATCCTAGAAGAGATTCTTATTAAAAGGTCCCAGCAGAAAAAGAAGACATCACCCT TAAACTACAAAGAGAGACTTTTTGTACTTACAAAATCCGTGTTGAGCTACTATGAGGGTCGAGCGGAGAA GAAATACAGAAAGGGCGTCATTGATATTTCCAAAATCAAGTGTGTGGAGATAGTGAAGAACGATGATGGT GTCATTCCCTGTCAAAATAAATTTCCATTCCAGGTTGTTCATGATGCTAATACACTTTATATTTTTGCAC CTAGTCCACAAAGCAGGGACCGATGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAATATCAT GATTAAATACCATCCTAAATTCTGGGCAGATGGGAGTTACCAGTGTTGTAGACAAACAGAAAAACTAGCA CCCGGATGTGAGAAGTACAATCTTTTTGAGAGTAGTATAAGAAAGACCCTGCCTCCCGCGCCAGAAATAA AGAAGAGAAGGCCTCCTCCACCAATTCCCCCAGAGGAAGAAAATACTGAAGAAATCGTTGTAGCGATGTA TGACTTCCAAGCGACGGAAGCACATGACCTCAGGTTAGAGAGAGGCCAAGAGTATATCATCCTGGAAAAG AATGACCTCCATTGGTGGAGAGCGAGAGATAAGTATGGGTGGTACTGCAGAAATACCAACAGAAGCAAAG CAGAACAGCTCCTCAGAACGGAAGATAAAGAAGGTGGTTTTATGGTGAGAGACTCCAGTCAACCAGGCTT GTACACTGTCTCCCTTTACACAAAGTTTGGGGGAGAAGGCTCATCAGGTTTCAGGCATTATCACATAAAG GAAACAGCAACATCCCCAAAGAAGTATTACCTGGCAGAGAAGCATGCTTTCGGGTCCATTCCTGAGATCA TTGAATATCACAAGCACAATGCGGCAGGGCTTGTCACCAGGCTGCGGTACCCGGTCAGTACAAAGGGGAA GAACGCTCCCACTACTGCCGGCTTCAGCTATGATAAGTGGGAGATTAACCCATCAGAGCTGACCTTTATG AGAGAGTTGGGGAGCGGACTGTTTGGAGTGGTGAGGCTTGGCAAGTGGCGGGCCCAGTACAAAGTGGCCA TCAAAGCTATCCGGGAAGGCGCCATGTGTGAAGAGGATTTCATAGAGGAAGCTAAAGTCATGATGAAGCT GACACACCCCAAGCTGGTACAGCTCTATGGTGTATGCACCCAGCAGAAGCCCATCTACATCGTTACCGAG TTCATGGAACGGGGCTGCCTTCTGAATTTCCTCCGGCAGAGACAAGGCCATTTCAGCAGAGACATGCTGC TAAGCATGTGTCAAGATGTCTGTGAAGGGATGGAGTACCTGGAGAGAAACAGCTTCATCCACAGAGACCT GGCTGCCAGAAATTGTCTAGTGAATGAAGCAGGAGTTGTCAAAGTATCTGATTTTGGAATGGCCAGGTAC GTTCTGGATGATCAGTACACAAGTTCTTCTGGCGCCAAGTTCCCTGTGAAGTGGTGTCCCCCAGAAGTGT TTAATTACAGCCGCTTTAGCAGCAAGTCAGACGTCTGGTCGTTTGGTGTGCTAATGTGGGAAATATTCAC AGAAGGCAGGATGCCCTTTGAGAAGAACACCAATTACGAAGTGGTAACCATGGTGACTCGTGGCCACCGC CTCCACCGGCCAAAGCTGGCTTCCAAATATTTGTATGAGGTGATGCTGAGATGCTGGCAAGAGAGACCAG AGGGAAGGCCTTCCTTTGAAGACTTGCTGCGTACGATAGATGAACTAGTTGAATGTGAAGAAACTTTTGG AAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209373 representing BC037071 Red=Cloning site Green=Tags(s) MNFNTILEEILIKRSQQKKKTSPLNYKERLFVLTKSVLSYYEGRAEKKYRKGVIDISKIKCVEIVKNDDG VIPCQNKFPFQVVHDANTLYIFAPSPQSRDRWVKKLKEEIKNNNNIMIKYHPKFWADGSYQCCRQTEKLA PGCEKYNLFESSIRKTLPPAPEIKKRRPPPPIPPEEENTEEIVVAMYDFQATEAHDLRLERGQEYIILEK NDLHWWRARDKYGWYCRNTNRSKAEQLLRTEDKEGGFMVRDSSQPGLYTVSLYTKFGGEGSSGFRHYHIK ETATSPKKYYLAEKHAFGSIPEIIEYHKHNAAGLVTRLRYPVSTKGKNAPTTAGFSYDKWEINPSELTFM RELGSGLFGVVRLGKWRAQYKVAIKAIREGAMCEEDFIEEAKVMMKLTHPKLVQLYGVCTQQKPIYIVTE FMERGCLLNFLRQRQGHFSRDMLLSMCQDVCEGMEYLERNSFIHRDLAARNCLVNEAGVVKVSDFGMARY VLDDQYTSSSGAKFPVKWCPPEVFNYSRFSSKSDVWSFGVLMWEIFTEGRMPFEKNTNYEVVTMVTRGHR LHRPKLASKYLYEVMLRCWQERPEGRPSFEDLLRTIDELVECEETFGR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC037071
ORF Size	1826 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC037071, AAH37071
RefSeq Size	2598 bp
RefSeq ORF	1826 bp
Locus ID	21682
Gene Summary	Non-receptor tyrosine kinase that contributes to signaling from many receptors and participates as a signal transducer in multiple downstream pathways, including regulation of the actin cytoskeleton. Plays a redundant role to ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Required for TCR-dependent IL2 gene induction. Phosphorylates DOK1, one CD28-specific substrate, and contributes to CD28-signaling. Mediates signals that negatively regulate IL2RA expression induced by TCR cross-linking. Plays a redundant role to BTK in BCR-signaling for B-cell development and activation, especially by phosphorylating STAP1, a BCR-signaling protein. Required in mast cells for efficient cytokine production. Involved in both growth and differentiation mechanisms of myeloid cells through activation by the granulocyte colony-stimulating factor CSF3, a critical cytokine to promoting the growth, differentiation, and functional activation of myeloid cells. Participates in platelet signaling downstream of integrin activation. Cooperates with JAK2 through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. GRB10, a negative modifier of the FOS activation pathway, is another substrate of TEC. TEC is involved in G protein-coupled receptor- and integrin-mediated signalings in blood platelets. Plays a role in hepatocyte proliferation and liver regeneration and is involved in HGF-induced ERK signaling pathway. TEC regulates also FGF2 unconventional secretion (endoplasmic reticulum (ER)/Golgi-independent mechanism) under various physiological conditions through phosphorylation of FGF2 'Tyr-82'. May also be involved in the regulation of osteoclast differentiation.[UniProtKB/Swiss-Prot Function]

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