Phactr1 (BC061691) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	9630030F18Rik; Rpel1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209331 representing BC061691 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTTCTTCGGAGGATGACATAGACCGGCGGCCCATCAGAAGAGTCCGCTCCAAGAGCGACACGC CATATCTCGCAGAGGCCAGGATCTCCTTTAACCTGGGGGCAGCCGAGGAAGTGGAGAGGCTGGCAGCCAT GCGTTCCGACTCGCTGGTCCCAGGTACGCACACCCCGCCCATCCGGAGGAGGAGTAAGTTTGCCAACCTG GGGAGGATTTTCAAACCCTGGAAATGGAGGAAGAAGAAAAGTGAAAAGTTCAAGCACACGTCAGCAGCTC TGGAAAGGAAGATTTCGATGAGGCAGAGCAGAGAGGAGCTGATCAAGAGAGGGGTCTTGAAGGAGATCTA CGATAAAGATGGAGAACTCTCTATATCAAATGAAGATGACTCCCTGGAGAACGGACAGTCCCTGAGCTCC AGCCAACTCTCTTTGCCTGCTCTGTCGGAAATGGAGCCTGTCCCAATGCCCAGGGACCCATGCTCATATG AGGTGCTCCAAGCTTCAGACATTATGGATGGACCAGTGTCTGAAGAGAGTCCCTCTGCCAGTGAGTCTGG AGTCCTCCTGTCCCAAGATCCTTCAGCCAAACCAGTTCTGTTCCTGCCCCCCAAAAAATCTGCTGCTTTC CCTGGAGACCATGAGGAGACCCCAGTGAAACAGTTGTCCCTTCACAAGCAGCCCCCGGCCCTGCCTCCCA AACCCACTGCCCGGATTGCCAACCACTTAACAGATCCTGGCGCCCCTGTGAAATTGCCTTGTCTGCCAGT GAAACTGTCGCCTCCGCTACCTCCAAAGAAAGTCCTGATCTGTATGCCTGTAGGGGGGCCAGAGCTCACT CTGGCATCCTATGCAGCCCAGAAGAGCAGCCAGCAGGCCGTGGCCCAGCACCACCACACCGTCCTACCAT CCCAGATGCAGCACCAGCTGCAGTATGGCAGTCACGGCCAGCATCTCCCATCCTCCACCGGCACCTTGCC CATGCACCCCTCAGGCTGCAGGATGATCGACGAGCTGAACAAGACACTCGCCATGACGATGCAGAGGCTG GAAAGCTCCGAGCAACGAGTCCCCTGTTCCACGTCTTACCACAGTTCTGGTTTGCACTCAAGCGACGGCA TCACAAAAGCAGGACCCATGGGCCTTCCGGAAATAAGACAAGTGCCAACTGTTGTGATTGAATGTGATGA CAATAAGGAAAATGTGCCTCATGAACCCGACTATGAAGACTCGCCCTGCCTGTATGGCCGGGAGGAGGAG GAAGAGGAGGAAGATGAAGATGATGACGCCTCTTTGTACACCAGCTCACTGGCCATGAAGGTGTGCAGGA AGGACTCCTTAGCCATCAAACTCAGCAACAGGCCCTCTAAGCGAGAGCTAGAAGAAAAGAACATCCTCCC CAGACAGACGGATGAGGAACGGCTGGAGCTCAGACAGCAGATTGGCACCAAGCTCACCAGGCGGCTGAGC CAGAGACCAACTGCAGAGGAACTGGAACAGAGGAACATTTTGAAGCCTCGGAATGAACAAGAAGAACAGG AGGAGAAGCGGGAAATCAAGAGGAGGCTGACTCGCAAGCTCAGCCAAAGGCCCACAGTGGAAGAACTCCG GGAGAGGAAGATCCTTATCCGCTTCAGTGACTACGTGGAAGTGGCAGATGCCCAAGACTATGACCGAAGG GCAGACAAGCCATGGACCCGCCTCACCGCTGCAGACAAAGCTGCCATTCGGAAGGAACTCAATGAGTTTA AAAGTACCGAGATGGAAGTCCACGAGTTAAGTAGACACTTAACCAGGTTTCATCGACCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209331 representing BC061691 Red=Cloning site Green=Tags(s) MAASSEDDIDRRPIRRVRSKSDTPYLAEARISFNLGAAEEVERLAAMRSDSLVPGTHTPPIRRRSKFANL GRIFKPWKWRKKKSEKFKHTSAALERKISMRQSREELIKRGVLKEIYDKDGELSISNEDDSLENGQSLSS SQLSLPALSEMEPVPMPRDPCSYEVLQASDIMDGPVSEESPSASESGVLLSQDPSAKPVLFLPPKKSAAF PGDHEETPVKQLSLHKQPPALPPKPTARIANHLTDPGAPVKLPCLPVKLSPPLPPKKVLICMPVGGPELT LASYAAQKSSQQAVAQHHHTVLPSQMQHQLQYGSHGQHLPSSTGTLPMHPSGCRMIDELNKTLAMTMQRL ESSEQRVPCSTSYHSSGLHSSDGITKAGPMGLPEIRQVPTVVIECDDNKENVPHEPDYEDSPCLYGREEE EEEEDEDDDASLYTSSLAMKVCRKDSLAIKLSNRPSKRELEEKNILPRQTDEERLELRQQIGTKLTRRLS QRPTAEELEQRNILKPRNEQEEQEEKREIKRRLTRKLSQRPTVEELRERKILIRFSDYVEVADAQDYDRR ADKPWTRLTAADKAAIRKELNEFKSTEMEVHELSRHLTRFHRP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC061691
ORF Size	1809 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC061691, AAH61691
RefSeq Size	5346 bp
RefSeq ORF	1811 bp
Locus ID	218194
Gene Summary	Binds actin monomers (G actin) and plays a role in multiple processes including the regulation of actin cytoskeleton dynamics, actin stress fibers formation, cell motility and survival, formation of tubules by endothelial cells, and regulation of PPP1CA activity. Involved in the regulation of cortical neuron migration and dendrite arborization (PubMed:30256902).[UniProtKB/Swiss-Prot Function]

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