Stxbp1 (BC031728) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Munc-18a, Munc18-1, MMS10-G, Ms10g, N-sec1, Rb-sec1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >BC031728 ORF sequence, MG209328 may differ due to SNPs. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCCCCATTGGCCTCAAGGCGGTGGTCGGAGAGAAGATCATGCATGATGTGATCAAGAAGGTGAAG AAGAAGGGCGAGTGGAAGGTGCTGGTGGTGGACCAGTTAAGCATGAGGATGCTGTCCTCCTGCTGCAAG ATGACAGACATCATGACCGAGGGCATCACAATTGTGGAGGATATCAACAAGCGCCGAGAGCCACTCCCC AGCCTGGAGGCCGTGTACCTCATCACCCCATCTGAGAAGTCTGTCCACTCTCTGATCAGTGATTTTAAG GACCCGCCGACTGCTAAATATCGGGCTGCGCATGTGTTCTTCACAGACTCGTGTCCAGATGCCCTATTT AACGAGCTGGTAAAATCCCGAGCAGCCAAAGTCATCAAGACGCTGACGGAAATCAACATTGCGTTTCTC CCCTATGAGTCCCAGGTGTATTCCCTGGACTCCGCTGACTCTTTCCAAAGCTTCTACAGCCCTCACAAG GCGCAGATGAAGAATCCGATACTGGAACGCCTGGCAGAGCAGATCGCAACCCTGTGTGCCACCCTGAAG GAGTATCCAGCTGTGCGGTATCGGGGGGAGTACAAGGACAATGCCTTGCTGGCTCAGCTGATCCAGGAC AAGCTGGATGCCTATAAAGCCGACGATCCAACAATGGGGGAGGGTCCCGACAAGGCACGGTCCCAGCTC CTGATCCTGGATCGTGGCTTTGACCCCAGCTCCCCTGTGCTCCATGAACTGACATTCCAGGCTATGAGT TATGACCTGCTGCCTATCGAAAATGATGTTTACAAGTATGAGACCAGCGGCATTGGAGAGGCGCGGGTG AAGGAGGTGCTACTGGATGAGGACGATGACCTGTGGATTGCGCTGCGACACAAGCACATCGCAGAGGTG TCCCAGGAAGTCACCCGGTCTCTGAAGGACTTTTCCTCTAGCAAGAGGATGAACACTGGCGAGAAGACC ACCATGCGGGACCTGTCCCAGATGCTGAAGAAAATGCCCCAGTACCAGAAGGAGCTCAGCAAGTATTCG ACTCACCTGCACCTTGCTGAAGACTGCATGAAGCATTACCAAGGCACTGTAGACAAACTCTGCCGCGTG GAGCAGGACCTGGCAATGGGCACAGATGCTGAGGGGGAAAAAATCAAGGACCCCATGAGAGCCATTGTC CCCATCCTGCTGGATGCGAACGTCAGCACTTACGACAAAATCCGTATCATCCTTCTCTACATCTTCCTG AAGAACGGTATCACTGAGGAGAACCTAAACAAACTCATCCAGCACGCTCAGATACCCCCAGAGGACAGC GAGATCATCACCAACATGGCTCACCTCGGCGTGCCCATCGTCACGGATTCCACACTACGCCGCCGAAGC AAACCGGAGCGGAAGGAGCGTATCAGTGAGCAGACCTACCAGCTCTCACGATGGACCCCGATCATTAAA GACATTATGGAGGACACTATCGAAGACAAGCTGGATACAAAGCACTACCCATACATCTCTACCCGCTCG TCCGCGTCCTTCAGCACCACTGCTGTGAGTGCCCGCTATGGACATTGGCACAAGAATAAGGCCCCCGGG GAGTACCGCAGCGGTCCCCGCCTCATTATTTTCATCCTTGGGGGTGTGAGCCTGAATGAGATGCGCTGT GCTTACGAAGTGACCCAGGCCAACGGCAAGTGGGAAGTGCTGATAGGTTCTACTCACATTCTCACTCCC ACCAAATTCCTCATGGACCTGAGACACCCCGACTTCAGGGAGTCCTCTAGGGTATCTTTTGAGGATCAG GCTCCAACAATGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC Protein Sequence (showhide) >Peptide sequence encoded by MG209328 Blue=ORF Red=Cloning site Green=Tag(s) MAPIGLKAVVGEKIMHDVIKKVKKKGEWKVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPLP SLEAVYLITPSEKSVHSLISDFKDPPTAKYRAAHVFFTDSCPDALFNELVKSRAAKVIKTLTEINIAFL PYESQVYSLDSADSFQSFYSPHKAQMKNPILERLAEQIATLCATLKEYPAVRYRGEYKDNALLAQLIQD KLDAYKADDPTMGEGPDKARSQLLILDRGFDPSSPVLHELTFQAMSYDLLPIENDVYKYETSGIGEARV KEVLLDEDDDLWIALRHKHIAEVSQEVTRSLKDFSSSKRMNTGEKTTMRDLSQMLKKMPQYQKELSKYS THLHLAEDCMKHYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRAIVPILLDANVSTYDKIRIILLYIFL KNGITEENLNKLIQHAQIPPEDSEIITNMAHLGVPIVTDSTLRRRSKPERKERISEQTYQLSRWTPIIK DIMEDTIEDKLDTKHYPYISTRSSASFSTTAVSARYGHWHKNKAPGEYRSGPRLIIFILGGVSLNEMRC AYEVTQANGKWEVLIGSTHILTPTKFLMDLRHPDFRESSRVSFEDQAPTME TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC031728
ORF Size	1809 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq Size	3617 bp
RefSeq ORF	1811 bp
Locus ID	20910
MW	68.7 kDa
Gene Summary	Participates in the regulation of synaptic vesicle docking and fusion through interaction with GTP-binding proteins (By similarity). Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions (By similarity).[UniProtKB/Swiss-Prot Function]

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