Dtnb (BC030924) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209321 representing BC030924 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTGAGGAAGGCGGGAACAAGCGGAAGACCATGGCGGAGAAGAGGCAGCTCTTCATAGAAATGCGTG CTCAGAATTTTGATGTCATACGACTATCAACGTACAGAACAGCTTGCAAGTTACGATTTGTACAGAAGCG ATGCAACCTTCATCTTGTTGATATCTGGAATATGATCGAAGCTTTCCGAGACAATGGCCTTAACACGCTG GACCACAGCACGGAGATCAGCGTGTCCCGCCTGGAGACCGTCATCTCGTCCATCTACTATCAGTTGAACA AGCGCCTTCCTTCTACTCACCAGATCAGCGTGGAGCAGTCCATCAGTCTCCTACTCAATTTCATGGTCGC CGCCTACGACAGTGAGGGCCGAGGCAAGTTGACCGTGTTTTCAGTTAAAGCTATGTTAGCAACCATGTGT GGTGGAAAAATGCTGGACAAATTGAGATACATTTTCTCCCAGATGTCAGATTCCAATGGCTTAATGATGT TTGGAAAGCTTGACCAGTTCCTGAAGGAAGCCCTGAAGCTCCCAACAGCTGTCTTTGAAGGGCCATCCTT TGGTTACACAGAGCATGCAGTCCGTACCTGTTTTCCGCAGCAGAAGAAGATAATGCTGAATATGTTTTTA GACACCATGATGGCTGATCCTCCTCCCCAGTGCCTTGTCTGGCTACCTCTCATGCACAGGCTTGCCCATG TTGAGAATGTCTTCCATCCTGTGGAATGCTCCTACTGTCACTGCGAGAGCATGATGGGCTTCCGATACCG ATGCCAGCAGTGCCACAACTACCAGCTCTGCCAGAACTGCTTTTGGCGTGGCCATGCCAGCGGCGCTCAC AGCAACCAGCACCAGATGAAGGAGCATTCCTCTTGGAAATCCCCTGCAAAGAAGCTGAGCCATGCAATTA GTAAATCTTTGGGATGTGTACCCTCCAGAGAACCCCCACATCCTGTTTTTCCTGAGCAACCAGAGAAACC ACTTGACCTTGCACATCTAGTTCCTCCTCGCCCTCTGACCAATATGAATGACACCGTGGTTAGTCACATG TCCTCTGGAGTGCCCACTCCCACCAAGAGGTTACAGTATAGCCAAGACATGCCCAATCTCTTGGCCGATG AGCATGCGCTGATAGCCTCCTATGTGGCTCGTCTGCAACACTGCACACGTGTGCTGGACAGTCCTAGCCG ACTGGATGAGGAACACCGGCTTATAGCCCGCTATGCTGCCCGGCTAGCTGCAGAGGCAGGAAACATGACT CGTCCTCCCACTGATGCCAGCTTCAACTTTGATGCCAACAAACAACAGAGACAGCTCATTGCAGAGCTGG AGAACAAGAACAGAGAGATCCTGCAGGAGATCCAGCGCCTGCGGCTGGAGCATGAACAGGCTTCCCAGCC CACCCCTGAGAAGGCTCAGCAGAACCCCATGCTGCTAGCAGAGCTGCGCTTGCTGAGGCAAAGGAAAGAT GAGCTGGAGCAAAGGATGTCAGCGCTGCAGGAGAGTAGGCGAGAGCTGATGGTGCAGCTGGAGGGGCTGA TGAAGTTGCTAAAGGCTCAAGCCACAGGGTCACCACACACATCACCCACCCATGGAGGTGGCCGCCCTAT GCCCATGCCTGTGCGTTCTACATCTGCTGGCTCCACCCCGACCCACGGCCCGCAGGACTCACTGAGTGGA GTTGGGGGAGATGTCCAGGAGGCCTTTGCACAAGGTACGAGGAGAAACCTCCGCAATGACTTGCTGGTGG CTGCTGACTCCATCACCAACACCATGTCCTCCCTGGTGAAGGAGCTCCATTCAGGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209321 representing BC030924 Red=Cloning site Green=Tags(s) MIEEGGNKRKTMAEKRQLFIEMRAQNFDVIRLSTYRTACKLRFVQKRCNLHLVDIWNMIEAFRDNGLNTL DHSTEISVSRLETVISSIYYQLNKRLPSTHQISVEQSISLLLNFMVAAYDSEGRGKLTVFSVKAMLATMC GGKMLDKLRYIFSQMSDSNGLMMFGKLDQFLKEALKLPTAVFEGPSFGYTEHAVRTCFPQQKKIMLNMFL DTMMADPPPQCLVWLPLMHRLAHVENVFHPVECSYCHCESMMGFRYRCQQCHNYQLCQNCFWRGHASGAH SNQHQMKEHSSWKSPAKKLSHAISKSLGCVPSREPPHPVFPEQPEKPLDLAHLVPPRPLTNMNDTVVSHM SSGVPTPTKRLQYSQDMPNLLADEHALIASYVARLQHCTRVLDSPSRLDEEHRLIARYAARLAAEAGNMT RPPTDASFNFDANKQQRQLIAELENKNREILQEIQRLRLEHEQASQPTPEKAQQNPMLLAELRLLRQRKD ELEQRMSALQESRRELMVQLEGLMKLLKAQATGSPHTSPTHGGGRPMPMPVRSTSAGSTPTHGPQDSLSG VGGDVQEAFAQGTRRNLRNDLLVAADSITNTMSSLVKELHSG TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC030924
ORF Size	1808 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC030924, AAH30924
RefSeq Size	2329 bp
RefSeq ORF	1808 bp
Locus ID	13528
Gene Summary	Scaffolding protein that assembles DMD and SNTA1 molecules to the basal membrane of kidney cells and liver sinusoids (PubMed:11585924). May function as a repressor of the SYN1 promoter through the binding of repressor element-1 (RE-1), in turn regulates SYN1 expression and may be involved in cell proliferation regulation during the early phase of neural differentiation (PubMed:20530487). May be required for proper maturation and function of a subset of inhibitory synapses (PubMed:16540561).[UniProtKB/Swiss-Prot Function]

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