Nr4a1 (NM_010444) Mouse Tagged ORF Clone

CAT#: MG209316

  • TrueORF®

Nr4a1 (tGFP-tagged) - Mouse nuclear receptor subfamily 4, group A, member 1 (Nr4a1)

ORF Plasmid: DDK tGFP



  "NM_010444" in other vectors (6)

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Mouse Protein / Lysate Request

CNY 6,080.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Gfrp; GFRP1; Hbr-1; Hbr1; Hmr; N10; NGFI-B; NGFIB; NP10; nur77; TIS1; TR3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209316 representing NM_010444
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTGTATTCAAGCTCAATATGGAACACCAGCAACGAGCCCAGGACCGCGTGACCACCTGACCGGTG
ATCCCCTGGCCCTTGAGTTCGGCAAGCCTACCATGGACCTGGCCAGCCCCGAGACAGCACCTGCCGCACC
TGCTACACTGCCCAGCTTCAGCACCTTCATGGACGGGTACACCGGAGAGTTTGACACCTTCCTCTACCAG
CTGCCGGGGACGACCCAGCCGTGCTCCTCAGCTTGTTCCTCTGCCTCCTCCACGTCTTCTTCCTCATCCT
CGGCCACCTCCCCCGCTTCGGCGTCCTTCAAGTTTGAGGACTTCCAGGTGTACGGCTGCTACCCGGGCAC
CCTGAGCGGCCCATTAGATGAGACCCTATCCTCCAGCGGCTCTGAGTACTATGGCAGTCCCTGCTCAGCC
CCCTCGCCATCTACACCCAACTTCCAGCCGTCCCAGCTTTCTCCCTGGGACGGCTCATTTGGCCACTTCT
CCCCGAGCCAGACTTATGAAGGCCTCTGGGCATGGACAGAGCAGTTGCCTAAGGCTTCTTCAGGGCCTCC
GCCACCTCCAACCTTCTTCTCCTTCAGTCCTCCCACTGGCCCCAGCCCCAGCCTGGCCCAGAGTTCTCTG
AAATTGTTCCCACCACCAGCCACCCACCAGCTTGGGGAGGGGGAGAGCTATTCCATGCCAGCAGCTTTCC
CCGGCTTGGCACCCACCTCTCCGAACCGTGACACTTCCGGCATTCTGGACGCACCCGTGACCTCCACCAA
GTCCCGGAGCGGGGCTTCAGGTGGCAGCGAGGGCCGCTGTGCAGTCTGTGGTGACAATGCTTCGTGTCAG
CACTATGGGGTCCGCACCTGTGAGGGCTGCAAGGGCTTCTTCAAGCGCACAGTACAGAAAAGCGCCAAGT
ACATCTGCCTGGCAAACAAGGATTGCCCTGTGGACAAGAGGCGGCGGAACCGCTGCCAGTTCTGCCGCTT
CCAGAAGTGCCTGGCTGTGGGCATGGTGAAGGAAGTTGTACGGACAGACAGCCTAAAAGGGCGGCGGGGC
CGGCTACCTTCAAAACCCAAGCAGCCTCCAGATGCCTCCCCTACCAATCTTCTCACTTCCCTCATCCGGG
CACACTTGGACTCCGGGCCTAGCACTGCCAAATTGGACTATTCCAAGTTCCAGGAACTGGTGCTGCCCCG
CTTCGGGAAGGAAGATGCCGGTGACGTGCAACAATTTTATGACTTGCTCTCTGGTTCCCTGGACGTTATC
CGAAAGTGGGCAGAAAAAATCCCTGGCTTCATTGAGCTTTGCCCAGGAGACCAAGACCTGTTGCTAGAGT
CTGCCTTCCTGGAACTCTTCATCCTCCGCCTGGCATACCGATCTAAACCCGGTGAGGGGAAGCTCATCTT
CTGCTCAGGCCTGGTACTACACCAGCTGCAGTGTGCCCGTGGCTTTGGTGATTGGATTGACAACATCCTG
GCCTTCTCACGGTCCCTGCACAGCTTGGGTGTTGATGTTCCCGCCTTTGCCTGCCTGTCCGCTCTGGTCC
TCATCACTGATCGACACGGGCTCCAGGACCCTCGTCGGGTGGAAGAGCTGCAGAATCGCATTGCTAGCTG
TCTGAAGGAGCACATGGCTACCGTGGCAGGAGACCCACAGCCGGCCAGCTGCCTGTCACGTCTGCTGGGC
AAACTGCCTGAGCTTCGGACCCTGTGCACTCAAGGCCTGCAGCGCATCTTTTGCCTCAAGTTGGAGGACT
TGGTACCCCCTCCACCTATTGTGGACAAGATCTTTATGGACACATTGTCTTTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209316 representing NM_010444
Red=Cloning site Green=Tags(s)

MPCIQAQYGTPATSPGPRDHLTGDPLALEFGKPTMDLASPETAPAAPATLPSFSTFMDGYTGEFDTFLYQ
LPGTTQPCSSACSSASSTSSSSSSATSPASASFKFEDFQVYGCYPGTLSGPLDETLSSSGSEYYGSPCSA
PSPSTPNFQPSQLSPWDGSFGHFSPSQTYEGLWAWTEQLPKASSGPPPPPTFFSFSPPTGPSPSLAQSSL
KLFPPPATHQLGEGESYSMPAAFPGLAPTSPNRDTSGILDAPVTSTKSRSGASGGSEGRCAVCGDNASCQ
HYGVRTCEGCKGFFKRTVQKSAKYICLANKDCPVDKRRRNRCQFCRFQKCLAVGMVKEVVRTDSLKGRRG
RLPSKPKQPPDASPTNLLTSLIRAHLDSGPSTAKLDYSKFQELVLPRFGKEDAGDVQQFYDLLSGSLDVI
RKWAEKIPGFIELCPGDQDLLLESAFLELFILRLAYRSKPGEGKLIFCSGLVLHQLQCARGFGDWIDNIL
AFSRSLHSLGVDVPAFACLSALVLITDRHGLQDPRRVEELQNRIASCLKEHMATVAGDPQPASCLSRLLG
KLPELRTLCTQGLQRIFCLKLEDLVPPPPIVDKIFMDTLSF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_010444
ORF Size 1803 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_010444.2
RefSeq Size 2456 bp
RefSeq ORF 1806 bp
Locus ID 15370
UniProt ID P12813
Gene Summary Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (By similarity). Plays a role in the vascular response to injury (PubMed:22427340).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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