Ppm1d (BC051966) Mouse Tagged ORF Clone

CAT#: MG209290

  • TrueORF®

Ppm1d (tGFP-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:62239 IMAGE:5689560)



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Mouse Protein / Lysate Request

CNY 6,056.00

CNY 6,560.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AV338790; Wip1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209290 representing BC051966
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGGCTGTACTCGCTGGGAGTGAGCGTCTTCTCGGACCAGGGCGGGAGGAAGTACATGGAGGACG
TAACTCAGATCGTGGTGGAGCCCGAGCCGGCTGCGGAGGACAAGCCGGCGCCGGTACCGCGGCGGGCGCT
CGGGTTGCCGGCGACCCCCACTCTCGCCGGCGTCGGGCCATCGGAAAAAGGCCCGATGGCGGCCCGCGAC
CCTGCCCCGGACGCCGCGGCCTCGCTACCCGCTGGCCGCTGCTGTCGCCGCCGCTCTTCGGTGGCCTTCT
TTGCAGTGTGCGACGGGCACGGCGGTCGGGAGGCGGCACAGTTTGCCCGGGAGCACTTGTGGGGTTTCAT
CAAGAAGCAGAAAGGCTTCACCTCGTCCGAGCCCGCCAAGGTGTGCGCTGCCATCCGCAAAGGTTTCCTC
GCCTGTCACCTCGCCATGTGGAAGAAACTGGCAGAATGGCCAAAGACTATGACAGGTCTTCCCAGCACGT
CCGGGACAACTGCCAGTGTGGTTATAATACGGGGCATGAAGATGTATGTAGCGCATGTAGGTGACTCTGG
GGTGGTCCTTGGAATTCAGGATGACCCAAAGGATGATTTTGTGAGAGCTGTGGAGGTGACACAAGATCAC
AAGCCAGAACTTCCCAAGGAAAGAGAAAGAATCGAAGGACTTGGCGGCAGTGTGATGAACAAGTCTGGAG
TGAACCGAGTAGTTTGGAAAAGGCCCCGGCTCACTCACAGTGGACCTGTCAGAAGGAGCACAGTCATTGA
CCAGATTCCTTTTCTGGCTGTAGCAAGAGCGCTTGGTGACTTGTGGAGCTATGATTTCTTCAGTGGTAAG
TTTGTGGTGTCACCTGAACCAGACACCAGTGTCCACACTCTTGACCCCCGGAAGCACAAGTATATTATCC
TGGGAAGTGATGGACTTTGGAATATGGTTCCACCTCAAGATGCCATCTCCATGTGCCAAGACCAAGAGGA
GAAAAAATACTTGATGGGTGAGCAAGGACAGTCCTGTGCCAAAATGCTTGTGAATCGAGCACTTGGCCGC
TGGAGGCAGCGTATGCTTCGGGCAGATAACACAAGTGCCATCGTAATCTGCATCTCTCCAGAAGTAGACA
ACCAAGGGAACTTCACCAATGAAGATGAGCTCTTTCTGAACCTGACTGATAGCCCTACTTACAACAGCCA
GGAGACCTGTGTGATGACTTCTTCTCCAAGTTCTACACCACCAATCAAGTCACCGGAAGAAGATGCATGG
CCAAGGCTGAGCTCTAAGGACCATATACCTGCCCTTGTTCGCAGTAATGCCTTCTCAGAGAAGTTTTTAG
AGGTCCCAGCTGAGATAGCTAGAGGGAATATCCAGACTGTAGTGATGACCTCAAAAGACTCAGAGACACT
TGAAGAAAATTGCCCCAAAGCCCTGACTTTAAGGATTCATGATTCTTTGAATAATACTCTGTCAGTTGGC
CTCATTCCAACCAATTCAACAAATACTATCATGGACCAAAAAAACTTAAAGATGTCAACTCCAGGTCAAA
TGAAAGCTCAAGAAGTTGAAAGAACCCCTCCAGCCAATTTTAAAAGGACATTAGAAGAATCCAACTCTGG
CCCCCTTATGAAGAAGCACCGACGAAATGGCTTAAGTCGAAGTAGCGGGGCCCAGGCTTCCAGTCTCCCT
ACAGCATCCCAGCGCAGGCACTCTGTCAAACTGACCCTGAGGCGCAGACTCAGGGGCCAGAGGAAGATGG
GAAATCCTCTTCTCCACCAGCACCGGAAAACAGTGTGTGTGTGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209290 representing BC051966
Red=Cloning site Green=Tags(s)

MAGLYSLGVSVFSDQGGRKYMEDVTQIVVEPEPAAEDKPAPVPRRALGLPATPTLAGVGPSEKGPMAARD
PAPDAAASLPAGRCCRRRSSVAFFAVCDGHGGREAAQFAREHLWGFIKKQKGFTSSEPAKVCAAIRKGFL
ACHLAMWKKLAEWPKTMTGLPSTSGTTASVVIIRGMKMYVAHVGDSGVVLGIQDDPKDDFVRAVEVTQDH
KPELPKERERIEGLGGSVMNKSGVNRVVWKRPRLTHSGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGK
FVVSPEPDTSVHTLDPRKHKYIILGSDGLWNMVPPQDAISMCQDQEEKKYLMGEQGQSCAKMLVNRALGR
WRQRMLRADNTSAIVICISPEVDNQGNFTNEDELFLNLTDSPTYNSQETCVMTSSPSSTPPIKSPEEDAW
PRLSSKDHIPALVRSNAFSEKFLEVPAEIARGNIQTVVMTSKDSETLEENCPKALTLRIHDSLNNTLSVG
LIPTNSTNTIMDQKNLKMSTPGQMKAQEVERTPPANFKRTLEESNSGPLMKKHRRNGLSRSSGAQASSLP
TASQRRHSVKLTLRRRLRGQRKMGNPLLHQHRKTVCVC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC051966
ORF Size 1794 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC051966, AAH51966
RefSeq Size 2908 bp
RefSeq ORF 1796 bp
Locus ID 53892
Gene Summary Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation (By similarity). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (PubMed:24135283).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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