Zfp143 (BC037658) Mouse Tagged ORF Clone

CAT#: MG209289

  • TrueORF®

Zfp143 (tGFP-tagged) - Mouse zinc finger protein 143 (cDNA clone MGC:46861 IMAGE:5356405)

ORF Plasmid: DDK tGFP



  "BC037658" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,800.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AA959806; AU015869; D7Ertd805e; KRAB14; pHZ-1; SBF; Staf; Zfp79; Zfp80-rs1; Znf143
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209289 representing BC037658
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGAATTTCCTGGAGGAGGAATGGAGGCTCAACATGTTACCCTGTGCCTGACGGAGGCAGTAACCG
TGGCAGATGGTGACAATTTAGAAAATATGGAAGGTGTGAGTTTACAAGCTGTGACACTCGCAGACGGCTC
TACTGCTTACATACAGCATAATTCTAAAGACGGGAGACTCATTGATGGCCAGGTCATTCAGCTGGAAGAT
GGTTCTGCTGCCTATGTTCAGCATGTCCCCATACCTAAAAGTAACAGTTATGACCAGAGCTCACTTCAGG
CCGTTCAGTTAGAAGATGGGACAACAGCTTATATCCACCATGCAGTGCAAGTCCCACAGTCTGATACCAT
CTTGGCAATTCAGGCTGATGGGACAGTGGCAGGGCTGCACACTGGGGATGCCACAATTGACCCAGATACC
ATTAGTGCTTTGGAGCAGTACGCAGCAAAGGTATCCATTGATGGCAGTGACGGTGTAACAAGTACAGGAA
TGATTGGAGAGAATGAGCAAGAGAAAAAAATGCAGATTGTCTTACAAGGCCATGCTACTCGGGTAACTCC
TAAATCTCAGCAGAGTGGAGAAAAGGCATTTCGGTGCAAATACGACGGCTGTGGGAAACTCTATACAACA
GCTCATCACCTTAAGGTCCATGAGCGCTCACACACAGGAGACCGGCCTTACCAGTGTGAGCATTCAGGCT
GTGGGAAAGCATTTGCAACAGGTTATGGATTAAAAAGTCATTTCAGAACTCATACTGGAGAAAAGCCATA
TCGGTGTTCAGAAGATAATTGTACAAAGTCTTTCAAAACTTCAGGAGATCTACAGAAGCACATCAGAACC
CATACAGGAGAAAGGCCCTTTAAGTGTCCTATTGAAGGCTGTGGTCGGTCCTTTACCACATCAAATATCA
GAAAAGTGCACATTAGGACACACACAGGAGAAAGACCTTATTACTGCACAGAACCTGGATGTGGGAGGGC
ATTTGCCAGCGCAACTAATTATAAAAACCATGTGAGGATACACACAGGAGAAAAGCCATATGTCTGTACA
GTTCCTGGGTGTGACAAACGGTTTACAGAATATTCCAGTTTGTATAAACACCATGTCGTTCACACTCACT
CTAAACCATACAACTGTAACCACTGTGGGAAGACGTACAAACAGATTTCCACACTGGCCATGCACAAACG
GACAGCCCACAACGACACAGAGCCCATTGAGGAGGAGCAGGAAGCCTTCTTCGAGCCTCCCCCAGGTCAA
GGTGATGATGTTCTTAAAGGGTCCCAGATCACATATGTTACAGGTGTAGATGGAGAGGACATTGTATCTA
CACAAGTAGCCACAGTAACCCAGTCTGGGCTGAGTCAACAAGTCACACTCATATCACAGGATGGGACTCA
ACATGTCAACATATCTCAAGCTGACATGCAGGCCATTGGCAACACCATCACCATGGTAACACAGGATGGC
ACACCCATCACGGTCCCCACTCACGATGCAGTCATCTCCTCAGCAGGAACACATTCTGTTGCTATGGTTA
CCGCGGAGGGCACAGAAGGACAGCAGGTCGCAATTGTAGCTCAAGATCTGGCAGCGTTCCATACAGCATC
TTCAGAGATGGGACACCAACAACATAGCCATCACCTAGTAACTACAGAAACCAGACCTCTGACCTTAGTG
GCAACATCCAACGGCACCCAGATCGCAGTTCAGCTTGGAGAGCAGCCGTCTCTGGAAGAAGCTATCAGAA
TAGCATCGAGGATCCAACAAGGGGAGACGCCAGGGTTGGATGAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209289 representing BC037658
Red=Cloning site Green=Tags(s)

MTEFPGGGMEAQHVTLCLTEAVTVADGDNLENMEGVSLQAVTLADGSTAYIQHNSKDGRLIDGQVIQLED
GSAAYVQHVPIPKSNSYDQSSLQAVQLEDGTTAYIHHAVQVPQSDTILAIQADGTVAGLHTGDATIDPDT
ISALEQYAAKVSIDGSDGVTSTGMIGENEQEKKMQIVLQGHATRVTPKSQQSGEKAFRCKYDGCGKLYTT
AHHLKVHERSHTGDRPYQCEHSGCGKAFATGYGLKSHFRTHTGEKPYRCSEDNCTKSFKTSGDLQKHIRT
HTGERPFKCPIEGCGRSFTTSNIRKVHIRTHTGERPYYCTEPGCGRAFASATNYKNHVRIHTGEKPYVCT
VPGCDKRFTEYSSLYKHHVVHTHSKPYNCNHCGKTYKQISTLAMHKRTAHNDTEPIEEEQEAFFEPPPGQ
GDDVLKGSQITYVTGVDGEDIVSTQVATVTQSGLSQQVTLISQDGTQHVNISQADMQAIGNTITMVTQDG
TPITVPTHDAVISSAGTHSVAMVTAEGTEGQQVAIVAQDLAAFHTASSEMGHQQHSHHLVTTETRPLTLV
ATSNGTQIAVQLGEQPSLEEAIRIASRIQQGETPGLDD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC037658
ORF Size 1794 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC037658.1
RefSeq Size 2980 bp
RefSeq ORF 1796 bp
Locus ID 20841
Gene Summary Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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