Lnpep (BC066791) Mouse Tagged ORF Clone

CAT#: MG209262

  • TrueORF®

Lnpep (tGFP-tagged) - Mouse leucyl/cystinyl aminopeptidase (cDNA clone MGC:76435 IMAGE:30054751)

ORF Plasmid: DDK tGFP



  "BC066791" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,800.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms IRAP, gp160, vp165, CAP, PLAP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209262 representing BC066791
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCGAAAACAGCATGTTTGAAGAAGAGCCAGATGTGGTAGATTTAGCCAAAGAACCTTGTTTACATC
CTCTGGAACCCGATGAAGTGGAATATGAGCCCCGAGGTTCGAGGCTTCTGGTGCGAGGTCTTGGTGAGCA
TGAGATGGACGAGGATGAAGAGGATTATGAGTCCTCTGCGAAGCTGCTGGGCATGTCCTTCATGAACAGA
AGCTCAGGCCTGCGGAACAGTGCAGCAGGCTACAGGCAGAGTCCAGATGGGACTTGTTCATTACCCTCTG
CCAGGACCTTAGTGATCTGTGTTTTTGTCATTGTGGTTGCGGTCTCTGTAATCATGGTGATTTATCTACT
GCCTAGATGTACCTTTACCAAAGAAGGCTGCCACAAAACAAACCAGTCAGCAGAACTCATCCAGCCAGTT
GCTACAAACGGGAAAGTGTTCCCATGGGCTCAAATTAGGCTTCCCACTGCCATTATTCCTCTATGCTATG
AACTTAGCCTACATCCAAACCTAACCTCAATGACATTCAGGGGATCTGTGACAATTTCACTTCAGGCTCT
TCAAGACACACGGGATATCATTCTCCATAGCACAGGACATAATATTTCAAGAGTGACATTTATGTCAGCA
GTTTCAAGTCAAGAAAAACAAGTTGAAATTCTGGAATATCCATATCATGAACAAATCGCCGTTGTTGCCC
CGGAACCTCTTCTAACAGGACACAATTATACCCTGAAGATAGAGTATTCAGCAAATATATCTAACTCTTA
TTATGGGTTTTATGGCATCACCTACACAGATAAAAGTAATGAGAAAAAGTACTTTGCAGCAACTCAGTTT
GAACCTCTGGCAGCAAGATCTGCTTTTCCTTGTTTTGATGAACCAGCATTTAAGGCCACATTTATCATCA
AGATCACAAGGAATGAGCACCATACTGCGTTATCAAATATGCCTAAGAAGTCATCGGTCCCTGCAGAAGA
AGGACTTATTCAAGATGAGTTTTCTGAAAGTGTGAAAATGAGCACATACCTGGTTGCTTTCATTGTAGGG
GAGATGAGGAACCTGAGTCAGGATGTAAATGGAACTCTGGTTTCTGTGTATGCTGTACCAGAAAAAATTG
GTCAAGTTCACCATGCCTTGGACACAACTATAAAGCTTCTTGAGTTTTATCAAACTTACTTTGAAATTCA
GTACCCACTTAAGAAATTGGATTTGGTGGCCATTCCTGACTTTGAAGCAGGAGCAATGGAAAACTGGGGC
CTGCTTACATTCCGAGAAGAGACTCTTCTGTATGACAATGCAACTTCTTCAGTAGCAGACAGAAAACTGG
TCACTAAAATCATTGCTCACGAACTGGCACATCAGTGGTTTGGAAATCTGGTTACAATGCAGTGGTGGAA
TGACCTGTGGCTAAATGAAGGCTTTGCCACTTTCATGGAGTATTTCTCTGTGGAAAAAATATTCAAAGAG
CTCAACAGTTATGAAGACTTCTTAGATGCTCGATTTAAAACCATGAGGAAAGATTCCTTGAATTCGTCTC
ATCCAATATCATCATCTGTTCAGTCTTCGGAACAAATAGAAGAAATGTTTGATTCTCTCTCCTATTTTAA
GGGAGCTTCTCTCTTGTTGATGCTGAAAAGTTACCTTAGTGAAGATGTATTTCGGCATGCTGTCATTCTT
TACCTGCACAATCACAGCTATGCAGCTATCCAAAGTGATGATCTCTGGGACAGCTTCAATGAGGTCACAG
ACAAAACTCTAGATGTAAAGAAAATGATGAAAACC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209262 representing BC066791
Red=Cloning site Green=Tags(s)

MIENSMFEEEPDVVDLAKEPCLHPLEPDEVEYEPRGSRLLVRGLGEHEMDEDEEDYESSAKLLGMSFMNR
SSGLRNSAAGYRQSPDGTCSLPSARTLVICVFVIVVAVSVIMVIYLLPRCTFTKEGCHKTNQSAELIQPV
ATNGKVFPWAQIRLPTAIIPLCYELSLHPNLTSMTFRGSVTISLQALQDTRDIILHSTGHNISRVTFMSA
VSSQEKQVEILEYPYHEQIAVVAPEPLLTGHNYTLKIEYSANISNSYYGFYGITYTDKSNEKKYFAATQF
EPLAARSAFPCFDEPAFKATFIIKITRNEHHTALSNMPKKSSVPAEEGLIQDEFSESVKMSTYLVAFIVG
EMRNLSQDVNGTLVSVYAVPEKIGQVHHALDTTIKLLEFYQTYFEIQYPLKKLDLVAIPDFEAGAMENWG
LLTFREETLLYDNATSSVADRKLVTKIIAHELAHQWFGNLVTMQWWNDLWLNEGFATFMEYFSVEKIFKE
LNSYEDFLDARFKTMRKDSLNSSHPISSSVQSSEQIEEMFDSLSYFKGASLLLMLKSYLSEDVFRHAVIL
YLHNHSYAAIQSDDLWDSFNEVTDKTLDVKKMMKT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC066791
ORF Size 1785 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC066791.1
RefSeq Size 2767 bp
RefSeq ORF 1787 bp
Locus ID 240028
Gene Summary Release of an N-terminal amino acid, cleave before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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