Numb (BC033459) Mouse Tagged ORF Clone

CAT#: MG209237

  • TrueORF®

Numb (tGFP-tagged) - Mouse numb gene homolog (Drosophila) (cDNA clone MGC:28234 IMAGE:3991630)

ORF Plasmid: DDK tGFP



  "BC033459" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,456.00

CNY 6,460.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Goat Polyclonal Antibody against NUMB
    • 100 ug

CNY 5,371.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms mnb, m-numb
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209237 representing BC033459
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAAACTACGGCAAAGCTTCAGGAGAAAGAAAGACGTTTATGTCCCAGAGGCCAGCCGTCCACATC
AGTGGCAGACAGATGAAGAAGGAGTCCGCACTGGAAAGTGTAGCTTCCCAGTTAAGTACCTCGGCCACGT
AGAAGTTGATGAGTCAAGAGGAATGCACATCTGTGAAGATGCCGTAAAGAGATTGAAAGCTACGGGAAAG
AAAGCAGTGAAGGCCGTTCTGTGGGTGTCAGCGGATGGGCTCAGAGTTGTGGACGAGAAAACTAAGGACC
TCATAGTTGACCAGACAATAGAAAAAGTTTCCTTCTGTGCCCCAGATAGGAACTTTGACAGAGCCTTTTC
TTACATATGTCGCGATGGCACCACTCGGCGATGGATCTGTCATTGTTTCATGGCTGTCAAAGACACGGGG
GAAAGACTGAGCCACGCCGTGGGCTGTGCTTTTGCAGCCTGTTTAGAGCGTAAACAGAAGCGGGAGAAGG
AGTGTGGCGTCACTGCTACTTTTGATGCCAGTAGAACCACTTTCACAAGAGAAGGATCATTCCGTGTCAC
AACTGCCACTGAGCAAGCCGAAAGAGAGGAGATCATGAAACAGTTGCAAGATGCCAAGAAAGCTGAGACA
GACAAGACAGCTGTTGGTCCATCAGTGGCTCCTGGCAACACTGCTCCATCCCCATCCTCTCCCACCTCTC
CCACTCCGGATGGCACTGCATCTTCAGAGATGAACAATCCCCATGCTATCCCACGCCGGCATGCACCAAT
TGAACAGCTTGCTCGTCAAGGCTCTTTCCGGGGATTTCCTGCTCTTAGCCAGAAGATGTCACCCTTTAAA
CGCCAGCTGTCCCTCCGCATCAATGAGTTGCCTTCCACTATGCAGAGGAAGACTGATTTCCCAATAAAAA
ACACAGTGCCCGAGGTGGAAGGAGAGGCCGAGAGCATCAGCTCCTTGTGTTCCCAGATCACCAGTGCCTT
CAGCACGCCCTCTGAGGACCCCTTCTCCTCCGCCCCAATGACCAAACCAGTGACATTGGTGGCACCACAG
TCTCCTGTGTTACAAGGGACTGAGTGGGGTCAGTCTTCTGGTGCTGCCTCTCCAGGTCTCTTCCAGGCTG
GTCACAGACGCACTCCCTCTGAAGCTGACCGCTGGTTAGAAGAAGTGTCAAAGAGTGTGCGGGCCCAGCA
GCCTCAGGTCTCAGCTGCCCCTCTGCAGCCAGTTCTCCAGCCGCCTCCGCCCGCCGCCATTGCCCCTCCA
GCACCTCCTTTCCAAGGACATGCCTTCCTCACGTCCCAGCCTGTGCCCGTGGGTGTGGTCCCACCCCTAC
AACCAGCCTTTGTCCCTACCCAGTCCTACCCTGTGGCCAACGGGATGCCCTACCCAGCCTCTAATGTGCC
TGTAGTGGGCATCACCCCATCCCAGATGGTAGCCAATGTGTTTGGCACTGCAGGCCACCCTCAGACCACT
CATCCACATCAGTCGCCAAGCCTGGCCAAGCAGCAGACATTCCCTCAATATGAGACAAGTAGTGCTACCA
CCAGTCCCTTCTTTAAGCCTCCTGCTCAGCACCTCAATGGTTCTGCAGCTTTCAATGGTGTAGACAATGG
TGGGCTAGCCTCAGGAAACAGGCATGCAGAAGTCCCTCCAGGCACCTGCCCAGTGGATCCTTTCGAAGCT
CAGTGGGCCGCACTAGAAAGCAAGTCCAAGCAGCGTACAAATCCTTCTCCTACCAACCCTTTCTCCAGTG
ACTTACAGAAAACGTTTGAAATAGAACTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209237 representing BC033459
Red=Cloning site Green=Tags(s)

MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYLGHVEVDESRGMHICEDAVKRLKATGK
KAVKAVLWVSADGLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRRWICHCFMAVKDTG
ERLSHAVGCAFAACLERKQKREKECGVTATFDASRTTFTREGSFRVTTATEQAEREEIMKQLQDAKKAET
DKTAVGPSVAPGNTAPSPSSPTSPTPDGTASSEMNNPHAIPRRHAPIEQLARQGSFRGFPALSQKMSPFK
RQLSLRINELPSTMQRKTDFPIKNTVPEVEGEAESISSLCSQITSAFSTPSEDPFSSAPMTKPVTLVAPQ
SPVLQGTEWGQSSGAASPGLFQAGHRRTPSEADRWLEEVSKSVRAQQPQVSAAPLQPVLQPPPPAAIAPP
APPFQGHAFLTSQPVPVGVVPPLQPAFVPTQSYPVANGMPYPASNVPVVGITPSQMVANVFGTAGHPQTT
HPHQSPSLAKQQTFPQYETSSATTSPFFKPPAQHLNGSAAFNGVDNGGLASGNRHAEVPPGTCPVDPFEA
QWAALESKSKQRTNPSPTNPFSSDLQKTFEIEL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC033459
ORF Size 1779 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC033459.1
RefSeq Size 3495 bp
RefSeq ORF 1781 bp
Locus ID 18222
Gene Summary This gene encodes a conserved protein that is distributed asymmetrically during cell division in the developing embryo. The encoded protein participates in cell fate decisions by interacting with the Notch receptor. Loss of function of this gene results in severe defects in neural development and loss of viability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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