Pdlim5 (BC037476) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Enh1, LIM, Enh
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209208 representing BC037476 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAACTACAGTGTGTCATTGGTCGGCCCAGCTCCTTGGGGTTTCCGGCTCCAAGGTGGCAAGGATT TCAACATGCCTCTGACAATCTCTAGTCTGAAGGATGGTGGCAAGGCATCTCAGGCACATGTCAGAATAGG CGACGTGGTTCTCAGCATCGATGGGATCAGTGCACAGGGAATGACGCATCTTGAAGCCCAGAACAAGATT AAGGCTTGTATGGGCTCCTTGAATATGACTCTACAAAGAGCTTCAGCTGCAGCCAAGAGTGAGCCGGTTT CCGTCCAGAAGGGTGAACCTAAGGAAGTAGTTAAGCCTGTGCCCATTACATCTCCTGCTGTATCCAAAGT CACTTCCACTACCAACATGGCCTACAATAAAGCACCACGGCCCTTTGGTTCTGTGTCTTCACCAAAAGTC ACATCCATCCCATCACCATCGTCTGCGTTCACCCCAGCCCATGCCGCCACTTCATCACATGCTTCCCCCA CACCTGTGGCCGCTGCCACTCCCCTCCACCTCTCTGCATCCGGACTGCATGTTAGTGCCAATCTTAGTGC TGACCAGTGTTCATCTCCACCGAACACTGGTAAACCTGCAGTTAATGTCCCACGGCAGCCCACAGTCACC AGCGTGTGTTCCGAGTCTGCTCAGGAGCTAGCAGAGGGACAGAGAAGAGGATCCCAGGGTGACATTAAGC AGCAAAATGGCCCACCAAGAAAACACATTGTGGAGCGCAACACGGAGTTTTATCACATACCCACTCACAG TGATGCCAGCAAGAAACGGCTGATTGAGGACACTGAGGACTGGCGCCCCCGGACTGGAACGACTCAGTCT CGTTCTTTCCGGATCCTTGCCCAGATCACTGGGACTGAGCATCTGACAGAATCTGAAAATGACAATACGA AGAAGGCAAATAGCACCCAGGAGCCTTCTCAGCAACCGGCGTCATCTGGAGCTTCACCTCTGAGCGCGTC TGAGGGCCCCGAGAGCCCAGGTTCCAGCAGGCCCTCGGTGGCTGGCCTCCGCTCCGCTGCTGCTTTCAAG CCTGTAGGATCCACCAGCGTCAAGTCACCTAGCTGGCAGCGTCCAAACCAAGCAGCACCTTCTACTGGGA GAATTTCAAACAATGCACGTTCTTCAGGTACAGGGGCGTCTGTGGGGCCTCCCCAGCCAAGTGACCAAGA CACACTTGTGCAGAGAGCGGAACACATTCCAGCGGGCAAGCGGACCCCCATGTGTGCCCACTGCAACCAA GTTATCAGGGGACCTTTTCTTGTGGCTCTGGGGAAGTCCTGGCATCCAGAAGAATTTAACTGTGCTCATT GCAAAAACACAATGGCCTACATAGGCTTTGTAGAGGAGAAGGGAGCACTGTACTGTGAGCTATGCTATGA GAAGTTCTTTGCTCCTGAATGTGGCCGGTGCCAAAGGAAGATCCTTGGAGAAGTCATCAATGCTTTGAAG CAGACCTGGCATGTGTCCTGTTTTGTGTGTGTGGCCTGTGGAAAACCCATTCGTAATAATGTTTTCCACT TGGAAGATGGCGAGCCCTACTGTGAGACAGACTACTACGCCCTTTTTGGAACAATATGTCGTGGATGTGA ATTCCCCATAGAGGCTGGTGACATGTTCCTGGAAGCTCTGGGATACACTTGGCATGATACTTGCTTTGTG TGCTCTGTGTGTTGTGAAAGTTTGGAAGGGCAGACATTTTTCTCCAAGAAGGACAAGCCACTTTGCAAAA AACATGCTCATTCTGTGAATTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209208 representing BC037476 Red=Cloning site Green=Tags(s) MSNYSVSLVGPAPWGFRLQGGKDFNMPLTISSLKDGGKASQAHVRIGDVVLSIDGISAQGMTHLEAQNKI KACMGSLNMTLQRASAAAKSEPVSVQKGEPKEVVKPVPITSPAVSKVTSTTNMAYNKAPRPFGSVSSPKV TSIPSPSSAFTPAHAATSSHASPTPVAAATPLHLSASGLHVSANLSADQCSSPPNTGKPAVNVPRQPTVT SVCSESAQELAEGQRRGSQGDIKQQNGPPRKHIVERNTEFYHIPTHSDASKKRLIEDTEDWRPRTGTTQS RSFRILAQITGTEHLTESENDNTKKANSTQEPSQQPASSGASPLSASEGPESPGSSRPSVAGLRSAAAFK PVGSTSVKSPSWQRPNQAAPSTGRISNNARSSGTGASVGPPQPSDQDTLVQRAEHIPAGKRTPMCAHCNQ VIRGPFLVALGKSWHPEEFNCAHCKNTMAYIGFVEEKGALYCELCYEKFFAPECGRCQRKILGEVINALK QTWHVSCFVCVACGKPIRNNVFHLEDGEPYCETDYYALFGTICRGCEFPIEAGDMFLEALGYTWHDTCFV CSVCCESLEGQTFFSKKDKPLCKKHAHSVNF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC037476
ORF Size	1775 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC037476, AAH37476
RefSeq Size	2382 bp
RefSeq ORF	1775 bp
Locus ID	56376
Gene Summary	May play an important role in the heart development by scaffolding PKC to the Z-disk region. Isoform 2 and isoform 3 may negatively modulate the scaffolding activity of isoform 1. May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses (By similarity).[UniProtKB/Swiss-Prot Function]

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